Q67YG7 · SOFL1_ARATH
- ProteinProtein SOB FIVE-LIKE 1
- GeneSOFL1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids148 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in cytokinin-mediated development (PubMed:20011053, PubMed:29467189).
Together with SOFL2, triggers the endogenous content of specific bioactive cytokinins derived from the biosynthetic intermediates trans-zeatin riboside monophosphate (tZRMP) and N6-(Delta2-isopentenyl)adenosine monophosphate (iPRMP) such as N-glucosides trans-zeatin 7-glucoside (tZ7G), cis-zeatin 7-glucoside (cZ7G) and N6-(Delta2-isopentenyl)adenine 7-glucoside (iP7G) (PubMed:20011053).
Together with SOFL2, triggers the endogenous content of specific bioactive cytokinins derived from the biosynthetic intermediates trans-zeatin riboside monophosphate (tZRMP) and N6-(Delta2-isopentenyl)adenosine monophosphate (iPRMP) such as N-glucosides trans-zeatin 7-glucoside (tZ7G), cis-zeatin 7-glucoside (cZ7G) and N6-(Delta2-isopentenyl)adenine 7-glucoside (iP7G) (PubMed:20011053).
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Biological Process | cytokinin biosynthetic process | |
Biological Process | cytokinin metabolic process | |
Biological Process | cytokinin-activated signaling pathway |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameProtein SOB FIVE-LIKE 1
- Short namesAtSOFL1
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ67YG7
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Phenotypes & Variants
Disruption phenotype
Plants missing both SOLF1 and SOLF2 have reduced endogenous cytokinin levels and accumulate lower levels of trans-zeatin riboside monophosphate (tZRMP) and N6-(Delta2-isopentenyl)adenosine monophosphate (iPRMP), biosynthetic intermediates of bioactive cytokinins as well as decreased response to exogenous cytokinin in both callus-formation and inhibition-of-hypocotyl-elongation assays.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 23 | Impaired function in cytokinin-mediated development; when associated with R-84. | ||||
Sequence: T → I | ||||||
Mutagenesis | 84 | Impaired function in cytokinin-mediated development; when associated with I-23. | ||||
Sequence: P → R |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 20 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000450249 | 1-148 | Protein SOB FIVE-LIKE 1 | |||
Sequence: MESPRNHGGSEEEEYSSCESGWTMYIEDAFHGNDQSSVVVDDDDDDTQVKEADDGYENDDGDTSDDGGDEESDDSMASDASSGPSNQLPKHINKHAARKNGSKQVYLQKRQHTEKTISNEGEKSDLKARTRTSAASRVQSRGKVSKTK |
Proteomic databases
Expression
Tissue specificity
Predominantly expressed in the vascular tissues of seedlings, developing leaves, flowers and siliques, but barely detectable in roots and stems.
Developmental stage
In seedlings, strong levels in the hydathode region of cotyledons as well as in the upper part of hypocotyls and weak content in the vascular tissue of cotyledons (PubMed:20011053).
In developing leaves, strongly expressed in the midrib of leaf veins, but weak levels in the hydathode regions (PubMed:20011053).
In developing flower buds, accumulates in pistil tips and the vascular tissue of stamens and sepals (PubMed:20011053).
In mature flowers, detected in the vascular bundles between the two anther locules, the central vascular cylinders of the filaments, vascular tissues of tips and bases of the pistils, and sepal vascular tissue (PubMed:20011053).
Also present in tips and bases of developing siliques, and progressively restricted to the vascular tissues at the silique tips (PubMed:20011053).
In developing leaves, strongly expressed in the midrib of leaf veins, but weak levels in the hydathode regions (PubMed:20011053).
In developing flower buds, accumulates in pistil tips and the vascular tissue of stamens and sepals (PubMed:20011053).
In mature flowers, detected in the vascular bundles between the two anther locules, the central vascular cylinders of the filaments, vascular tissues of tips and bases of the pistils, and sepal vascular tissue (PubMed:20011053).
Also present in tips and bases of developing siliques, and progressively restricted to the vascular tissues at the silique tips (PubMed:20011053).
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, motif, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-20 | Disordered | ||||
Sequence: MESPRNHGGSEEEEYSSCES | ||||||
Motif | 20-25 | SOFL-A | ||||
Sequence: SGWTMY | ||||||
Region | 33-148 | Disordered | ||||
Sequence: NDQSSVVVDDDDDDTQVKEADDGYENDDGDTSDDGGDEESDDSMASDASSGPSNQLPKHINKHAARKNGSKQVYLQKRQHTEKTISNEGEKSDLKARTRTSAASRVQSRGKVSKTK | ||||||
Compositional bias | 39-76 | Acidic residues | ||||
Sequence: VVDDDDDDTQVKEADDGYENDDGDTSDDGGDEESDDSM | ||||||
Motif | 75-84 | SOFL-B | ||||
Sequence: SMASDASSGP | ||||||
Compositional bias | 108-130 | Basic and acidic residues | ||||
Sequence: QKRQHTEKTISNEGEKSDLKART |
Domain
Domains SOFL-A and SOFL-B are required for function in cytokinin-mediated development.
Sequence similarities
Belongs to the SOFL plant protein family.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q67YG7-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length148
- Mass (Da)16,249
- Last updated2004-10-11 v1
- ChecksumC0DD7F92C3A745E0
Q67YG7-2
- Name2
Sequence caution
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 35 | in Ref. 3; BAD43473 | ||||
Sequence: Q → L | ||||||
Compositional bias | 39-76 | Acidic residues | ||||
Sequence: VVDDDDDDTQVKEADDGYENDDGDTSDDGGDEESDDSM | ||||||
Sequence conflict | 59 | in Ref. 5; AAM65051 | ||||
Sequence: D → N | ||||||
Compositional bias | 108-130 | Basic and acidic residues | ||||
Sequence: QKRQHTEKTISNEGEKSDLKART |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC079829 EMBL· GenBank· DDBJ | AAG50669.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002684 EMBL· GenBank· DDBJ | AEE30661.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK175479 EMBL· GenBank· DDBJ | BAD43242.1 EMBL· GenBank· DDBJ | mRNA | ||
AK175710 EMBL· GenBank· DDBJ | BAD43473.1 EMBL· GenBank· DDBJ | mRNA | ||
AK176501 EMBL· GenBank· DDBJ | BAD44264.1 EMBL· GenBank· DDBJ | mRNA | ||
BT024679 EMBL· GenBank· DDBJ | ABD57504.1 EMBL· GenBank· DDBJ | mRNA | ||
AY087508 EMBL· GenBank· DDBJ | AAM65051.1 EMBL· GenBank· DDBJ | mRNA |