Q67Y99 · CLPF_ARATH
- ProteinClp protease adapter protein ClpF, chloroplastic
- GeneCLPF
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids330 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Clp protease adapter that facilitates CLPS1 recruitment to ClpC chaperones thus forming a binary adapter for selective substrate recognition and delivery to plastid Clp protease system (CLPC).
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | chloroplastic endopeptidase Clp complex | |
Molecular Function | DNA binding |
Names & Taxonomy
Protein names
- Recommended nameClp protease adapter protein ClpF, chloroplastic
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ67Y99
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Reduction by 10 precent of total chlorophyll, with slight increase of chlorophyll a/b ratio and slight decrease of chlorophyll-to-carotenoid ratios. Overaccumulation of ClpS1 protein.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 44 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-65 | Chloroplast | ||||
Sequence: MVQSQSLSTLTICGSVKVSSLLRNRLNSVKASSLIGDRCVSCQFLRKSPSFRSHWKSLKQRNLLR | ||||||
Chain | PRO_0000438516 | 66-330 | Clp protease adapter protein ClpF, chloroplastic | |||
Sequence: VEARWPFQGGGEQGLDPSSERSESANEDILIFFFQLDLATRVQYAMNLEQYDIAQQLREKLTEVEEESIRLQEGKRGSSAKSEAQDKGISIIRLRADLQNAIDSEDYGLAAKLRDEISKLEAESLAVSAKALAFEKAEYAFRLGQKLRHKTFGYRAVVCGMDPICSESSSWMEAAEVEKLPRGSNQPFYQVLVDVRTHPDLLVAYVAEDNLLAPEKPDKERFDHPYISFLYYGADTAGDFIPVKQLREKYNRPRHEVPFDSQDED |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed constitutively in photosynthetic tissues such as leaves, stems and flowers, and, at low levels, in siliques.
Developmental stage
Slight reduction during senescence.
Gene expression databases
Interaction
Subunit
Binds to CLPC1 and CLPC2. Interacts with ClpS1; this interaction stimulates their association with ClpC. Associates with the Clp substrate HEMA1 (GluTR).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, coiled coil, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 66-138 | NTD, required for CLPS1-binding | ||||
Sequence: VEARWPFQGGGEQGLDPSSERSESANEDILIFFFQLDLATRVQYAMNLEQYDIAQQLREKLTEVEEESIRLQE | ||||||
Coiled coil | 112-139 | |||||
Sequence: NLEQYDIAQQLREKLTEVEEESIRLQEG | ||||||
Domain | 153-188 | UVR | ||||
Sequence: GISIIRLRADLQNAIDSEDYGLAAKLRDEISKLEAE | ||||||
Coiled coil | 175-195 | |||||
Sequence: AAKLRDEISKLEAESLAVSAK | ||||||
Region | 203-310 | YccV-like | ||||
Sequence: EYAFRLGQKLRHKTFGYRAVVCGMDPICSESSSWMEAAEVEKLPRGSNQPFYQVLVDVRTHPDLLVAYVAEDNLLAPEKPDKERFDHPYISFLYYGADTAGDFIPVKQ |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 4 isoforms produced by Alternative splicing.
Q67Y99-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length330
- Mass (Da)37,440
- Last updated2004-10-11 v1
- Checksum86A3998711AD4A11
Q67Y99-2
- Name2
- Differences from canonical
- 1-110: Missing
Q67Y99-3
- Name3
- Differences from canonical
- 1-217: MVQSQSLSTLTICGSVKVSSLLRNRLNSVKASSLIGDRCVSCQFLRKSPSFRSHWKSLKQRNLLRVEARWPFQGGGEQGLDPSSERSESANEDILIFFFQLDLATRVQYAMNLEQYDIAQQLREKLTEVEEESIRLQEGKRGSSAKSEAQDKGISIIRLRADLQNAIDSEDYGLAAKLRDEISKLEAESLAVSAKALAFEKAEYAFRLGQKLRHKTF → MYYLSACLTLVYIHNEELMLVSFRL
Q67Y99-4
- Name4
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1P8B2M3 | A0A1P8B2M3_ARATH | At2g03390 | 273 | ||
A0A1P8B2M4 | A0A1P8B2M4_ARATH | At2g03390 | 266 |
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_058669 | 1-110 | in isoform 2 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_058668 | 1-217 | in isoform 3 | |||
Sequence: MVQSQSLSTLTICGSVKVSSLLRNRLNSVKASSLIGDRCVSCQFLRKSPSFRSHWKSLKQRNLLRVEARWPFQGGGEQGLDPSSERSESANEDILIFFFQLDLATRVQYAMNLEQYDIAQQLREKLTEVEEESIRLQEGKRGSSAKSEAQDKGISIIRLRADLQNAIDSEDYGLAAKLRDEISKLEAESLAVSAKALAFEKAEYAFRLGQKLRHKTF → MYYLSACLTLVYIHNEELMLVSFRL | ||||||
Alternative sequence | VSP_058670 | 109-131 | in isoform 4 | |||
Sequence: YAMNLEQYDIAQQLREKLTEVEE → VKTKCRVFISFKMVANGFDVLQY | ||||||
Alternative sequence | VSP_058671 | 132-330 | in isoform 4 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC006284 EMBL· GenBank· DDBJ | AAD17436.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AC006284 EMBL· GenBank· DDBJ | AAD17448.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | AEC05695.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | AEC05696.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | ANM63152.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY128399 EMBL· GenBank· DDBJ | AAM91602.1 EMBL· GenBank· DDBJ | mRNA | ||
BT000080 EMBL· GenBank· DDBJ | AAN15399.1 EMBL· GenBank· DDBJ | mRNA | ||
BT012279 EMBL· GenBank· DDBJ | AAS76766.1 EMBL· GenBank· DDBJ | mRNA | ||
AK176535 EMBL· GenBank· DDBJ | BAD44298.1 EMBL· GenBank· DDBJ | mRNA | ||
AK176569 EMBL· GenBank· DDBJ | BAD44332.1 EMBL· GenBank· DDBJ | mRNA |