Q65ZG8 · PER_CAEEL
- ProteinPeriod protein homolog lin-42
- Genelin-42
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids597 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcriptional repressor which interacts with the promoter region of target genes (PubMed:25032706).
Has a specific role in developmental timing where it regulates temporal expression of a number of miRNAs and mRNAs (PubMed:15691769, PubMed:16300753, PubMed:25032706, PubMed:25319259, PubMed:29880558).
Controls temporal cell fate transition during embryonic and early larval development by restricting the expression of specific miRNAs, including let-7, miR-48, lin-4, miR-35 and miR-58 (PubMed:15691769, PubMed:24699545, PubMed:25032706, PubMed:25319259, PubMed:29880558).
Restricts the accumulation of lin-29 in the hypodermis to the larval L4 stage, thus controlling terminal differentiation of seam cells (PubMed:10550049, PubMed:21471153).
Has a role in the miRNA-mediated specification of asymmetric gene expression patterns in gustatory neurons (PubMed:25032706).
May also regulate genes involved in other biological processes including transport, small molecule metabolism, and growth (PubMed:25032706).
Inhibits dauer formation, by antagonizing daf-12 (PubMed:20843862).
Has a specific role in developmental timing where it regulates temporal expression of a number of miRNAs and mRNAs (PubMed:15691769, PubMed:16300753, PubMed:25032706, PubMed:25319259, PubMed:29880558).
Controls temporal cell fate transition during embryonic and early larval development by restricting the expression of specific miRNAs, including let-7, miR-48, lin-4, miR-35 and miR-58 (PubMed:15691769, PubMed:24699545, PubMed:25032706, PubMed:25319259, PubMed:29880558).
Restricts the accumulation of lin-29 in the hypodermis to the larval L4 stage, thus controlling terminal differentiation of seam cells (PubMed:10550049, PubMed:21471153).
Has a role in the miRNA-mediated specification of asymmetric gene expression patterns in gustatory neurons (PubMed:25032706).
May also regulate genes involved in other biological processes including transport, small molecule metabolism, and growth (PubMed:25032706).
Inhibits dauer formation, by antagonizing daf-12 (PubMed:20843862).
Isoform a
Specifically required for maintaining the timing of larval development and molting cycle rhythms.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | RNA polymerase II-specific DNA-binding transcription factor binding | |
Biological Process | cell differentiation | |
Biological Process | negative regulation of dauer larval development | |
Biological Process | negative regulation of miRNA transcription | |
Biological Process | regulation of development, heterochronic |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePeriod protein homolog lin-42
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ65ZG8
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Mutants exhibit a delay in development characterized by a strong molting defect, resulting in a prolonged L1 stage followed by an arrest in development in the L2 stage in most animals (PubMed:25319259).
There is increased expression of the heterochronic miRNAs, lin-4 and miR-48, in late L1 (PubMed:25319259).
In addition, there is increased expression of the heterochronic miRNA let-7 at the L3 and L4 larval stages (PubMed:29880558).
Seam cell shape and connectivity is severely abnormal during the molt stages (PubMed:22137474).
Animals display a precocious alae phenotype, with the early formation of either full or partial alae in the adult cuticle (PubMed:25319259, PubMed:29880558).
The precocious alae phenotype is suppressed in the double lin-42 and let-7 mutant and lin-42 and miR-48 mutant (PubMed:25319259).
The quadruple lin-42, miR-48, miR-241 and let-7 mutant has a retarded phenotype at L4, with many animals having few full and partial alae or none (PubMed:25319259).
The number of animals displaying a squat body statue, referred to as a dumpy phenotype, is increased, the incomplete alae formation defect is rescued and increased let-7 levels are reduced in a kin-20 RNAi-mediated knockdown mutant background (PubMed:29880558).
RNAi-mediated knockdown leads to a severe hypodermal phenotype, premature execution of developmental events in vulval precursor cells, DTC and sex myoblasts (PubMed:16300753).
Furthermore, seam cell terminal differentiation is only partially initiated at the L4 larval stage (PubMed:15691769).
RNAi-mediated knockdown increases the survival rate and partially restores alae formation of let-7 n2853 mutants at 20 degrees Celsius (PubMed:15691769).
There is increased expression of the heterochronic miRNAs, lin-4 and miR-48, in late L1 (PubMed:25319259).
In addition, there is increased expression of the heterochronic miRNA let-7 at the L3 and L4 larval stages (PubMed:29880558).
Seam cell shape and connectivity is severely abnormal during the molt stages (PubMed:22137474).
Animals display a precocious alae phenotype, with the early formation of either full or partial alae in the adult cuticle (PubMed:25319259, PubMed:29880558).
The precocious alae phenotype is suppressed in the double lin-42 and let-7 mutant and lin-42 and miR-48 mutant (PubMed:25319259).
The quadruple lin-42, miR-48, miR-241 and let-7 mutant has a retarded phenotype at L4, with many animals having few full and partial alae or none (PubMed:25319259).
The number of animals displaying a squat body statue, referred to as a dumpy phenotype, is increased, the incomplete alae formation defect is rescued and increased let-7 levels are reduced in a kin-20 RNAi-mediated knockdown mutant background (PubMed:29880558).
RNAi-mediated knockdown leads to a severe hypodermal phenotype, premature execution of developmental events in vulval precursor cells, DTC and sex myoblasts (PubMed:16300753).
Furthermore, seam cell terminal differentiation is only partially initiated at the L4 larval stage (PubMed:15691769).
RNAi-mediated knockdown increases the survival rate and partially restores alae formation of let-7 n2853 mutants at 20 degrees Celsius (PubMed:15691769).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000432385 | 1-597 | Period protein homolog lin-42 | |||
Sequence: MEPAGHSSATHNIVVPNANPTQPQPLAPAMREEGATLSPPNTWSSSSVEFLDDADDNRLLFTCTFTLPHGTVLSSATYADGFHEQYLTIGDNFLARLEPKGQSFILSAAAASVKQRIFARVTMPDGALRACELLCEFETDRAKITVLALRSAFSLQASHVSSNFHVFTFITKHSSTCALTHIDYASIPYLGLLPTDLIGKSLLAFVYSPDVHVVRQAHIDLHNSRGKIVKSIADLRLVAHNGSILRCQTEWSAYVNPWTRKMELVVARHRICSLPIGDSDVISSPPPGIQSNTLPPVMAKTFEDELRTIMNKPVPSTSRHSHHHHHSSLKDQNQGFPANIDLGAYIDKIVEQLVVNSTAQQQQKVAVAAAAAAQAAQAAVVATAQIRKVASAPPTTSTDPPLSYTQINCLENVHRLLKSQSRPESPAKQDEPFDEKKYPPQTPLTREALTLHTKRFEDEYKDTWCRRLKRLSDDVPSSPPAKRTTPIHWTSSSQNHYRTMAPAPPPPPGKNYQITYTPLDDLTDQKSTNTKSDVENVAYPISGSKFSTPMRLSIDGLLPRGATSTGGASPTSGTNSPPVFPKTSSSSSLLMLRDSQN |
Proteomic databases
Expression
Developmental stage
Expressed from late embryonic stage onwards (PubMed:10550049, PubMed:16300753).
Shows oscillating expression levels during larval stages, with peak levels in intermolt periods and minimal levels during ecdysis (PubMed:16300753, PubMed:20843862, PubMed:22137474, PubMed:29880558).
Expressed in hypodermis, vulva, intestine, muscle, neurons and somatic gonad throughout larval development, in an oscillating pattern (PubMed:16300753, PubMed:22137474).
Expressed in sex myoblasts during larval stages L1, L2 and L3, in an oscillating pattern (PubMed:16300753).
Expressed in gonad distal tip cells (DTC) during the L2 and L3 stages, in an oscillating pattern (PubMed:16300753).
Shows oscillating expression levels during larval stages, with peak levels in intermolt periods and minimal levels during ecdysis (PubMed:16300753, PubMed:20843862, PubMed:22137474, PubMed:29880558).
Expressed in hypodermis, vulva, intestine, muscle, neurons and somatic gonad throughout larval development, in an oscillating pattern (PubMed:16300753, PubMed:22137474).
Expressed in sex myoblasts during larval stages L1, L2 and L3, in an oscillating pattern (PubMed:16300753).
Expressed in gonad distal tip cells (DTC) during the L2 and L3 stages, in an oscillating pattern (PubMed:16300753).
Isoform a
Expressed in the pharyngeal myoepithelium, the major body hypodermal syncytium and lateral seam cells from the mid L1 stage. Expression in the hypodermis rises and falls at the start and end of every molt, respectively.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, domain, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-44 | Disordered | ||||
Sequence: MEPAGHSSATHNIVVPNANPTQPQPLAPAMREEGATLSPPNTWS | ||||||
Domain | 155-223 | PAS | ||||
Sequence: LQASHVSSNFHVFTFITKHSSTCALTHIDYASIPYLGLLPTDLIGKSLLAFVYSPDVHVVRQAHIDLHN | ||||||
Region | 313-335 | Disordered | ||||
Sequence: PVPSTSRHSHHHHHSSLKDQNQG | ||||||
Region | 418-450 | Disordered | ||||
Sequence: KSQSRPESPAKQDEPFDEKKYPPQTPLTREALT | ||||||
Compositional bias | 424-441 | Basic and acidic residues | ||||
Sequence: ESPAKQDEPFDEKKYPPQ | ||||||
Region | 473-509 | Disordered | ||||
Sequence: DDVPSSPPAKRTTPIHWTSSSQNHYRTMAPAPPPPPG | ||||||
Compositional bias | 483-499 | Polar residues | ||||
Sequence: RTTPIHWTSSSQNHYRT | ||||||
Region | 555-597 | Disordered | ||||
Sequence: DGLLPRGATSTGGASPTSGTNSPPVFPKTSSSSSLLMLRDSQN | ||||||
Compositional bias | 564-597 | Polar residues | ||||
Sequence: STGGASPTSGTNSPPVFPKTSSSSSLLMLRDSQN |
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative promoter usage & Alternative splicing. Additional isoforms seem to exist.
Q65ZG8-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Nameb
- Synonymsc
- Length597
- Mass (Da)65,255
- Last updated2004-10-11 v1
- Checksum26ED5D4061A5CA43
Q65ZG8-2
- Namea
- Synonymsd
- NoteProduced by alternative promoter usage.
- Differences from canonical
- 1-312: MEPAGHSSATHNIVVPNANPTQPQPLAPAMREEGATLSPPNTWSSSSVEFLDDADDNRLLFTCTFTLPHGTVLSSATYADGFHEQYLTIGDNFLARLEPKGQSFILSAAAASVKQRIFARVTMPDGALRACELLCEFETDRAKITVLALRSAFSLQASHVSSNFHVFTFITKHSSTCALTHIDYASIPYLGLLPTDLIGKSLLAFVYSPDVHVVRQAHIDLHNSRGKIVKSIADLRLVAHNGSILRCQTEWSAYVNPWTRKMELVVARHRICSLPIGDSDVISSPPPGIQSNTLPPVMAKTFEDELRTIMNK → MDILSYLYDLAE
Q65ZG8-3
- Namec
- Synonymsa
- NoteProduced by alternative splicing.
- Differences from canonical
- 313-453: PVPSTSRHSHHHHHSSLKDQNQGFPANIDLGAYIDKIVEQLVVNSTAQQQQKVAVAAAAAAQAAQAAVVATAQIRKVASAPPTTSTDPPLSYTQINCLENVHRLLKSQSRPESPAKQDEPFDEKKYPPQTPLTREALTLHT → VSSAISSVTFLSSGDGASHRLSSSSVLLVLTLHFYLCASLRRDTRLARRIRRRESGQQRHLSEQKVHMLALRSMAPCQEKECKNDALAGGAVSGTTDELIPRRTFLEFAWNGECNFIGGPVLYGSRRDMVQVLQKPTKLKS
- 454-597: Missing
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A131MBE1 | A0A131MBE1_CAEEL | lin-42 | 510 | ||
A0A131MAY5 | A0A131MAY5_CAEEL | lin-42 | 654 | ||
A0A131MAT8 | A0A131MAT8_CAEEL | lin-42 | 336 | ||
A0A131MCB3 | A0A131MCB3_CAEEL | lin-42 | 98 |
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_057503 | 1-312 | in isoform a | |||
Sequence: MEPAGHSSATHNIVVPNANPTQPQPLAPAMREEGATLSPPNTWSSSSVEFLDDADDNRLLFTCTFTLPHGTVLSSATYADGFHEQYLTIGDNFLARLEPKGQSFILSAAAASVKQRIFARVTMPDGALRACELLCEFETDRAKITVLALRSAFSLQASHVSSNFHVFTFITKHSSTCALTHIDYASIPYLGLLPTDLIGKSLLAFVYSPDVHVVRQAHIDLHNSRGKIVKSIADLRLVAHNGSILRCQTEWSAYVNPWTRKMELVVARHRICSLPIGDSDVISSPPPGIQSNTLPPVMAKTFEDELRTIMNK → MDILSYLYDLAE | ||||||
Alternative sequence | VSP_057504 | 313-453 | in isoform c | |||
Sequence: PVPSTSRHSHHHHHSSLKDQNQGFPANIDLGAYIDKIVEQLVVNSTAQQQQKVAVAAAAAAQAAQAAVVATAQIRKVASAPPTTSTDPPLSYTQINCLENVHRLLKSQSRPESPAKQDEPFDEKKYPPQTPLTREALTLHT → VSSAISSVTFLSSGDGASHRLSSSSVLLVLTLHFYLCASLRRDTRLARRIRRRESGQQRHLSEQKVHMLALRSMAPCQEKECKNDALAGGAVSGTTDELIPRRTFLEFAWNGECNFIGGPVLYGSRRDMVQVLQKPTKLKS | ||||||
Compositional bias | 424-441 | Basic and acidic residues | ||||
Sequence: ESPAKQDEPFDEKKYPPQ | ||||||
Alternative sequence | VSP_057505 | 454-597 | in isoform c | |||
Sequence: Missing | ||||||
Compositional bias | 483-499 | Polar residues | ||||
Sequence: RTTPIHWTSSSQNHYRT | ||||||
Compositional bias | 564-597 | Polar residues | ||||
Sequence: STGGASPTSGTNSPPVFPKTSSSSSLLMLRDSQN |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF183400 EMBL· GenBank· DDBJ | AAF13188.1 EMBL· GenBank· DDBJ | mRNA | ||
BX284602 EMBL· GenBank· DDBJ | CCD71436.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CCD71435.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CCD71437.1 EMBL· GenBank· DDBJ | Genomic DNA |