Q65ZG8 · PER_CAEEL

  • Protein
    Period protein homolog lin-42
  • Gene
    lin-42
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Transcriptional repressor which interacts with the promoter region of target genes (PubMed:25032706).
Has a specific role in developmental timing where it regulates temporal expression of a number of miRNAs and mRNAs (PubMed:15691769, PubMed:16300753, PubMed:25032706, PubMed:25319259, PubMed:29880558).
Controls temporal cell fate transition during embryonic and early larval development by restricting the expression of specific miRNAs, including let-7, miR-48, lin-4, miR-35 and miR-58 (PubMed:15691769, PubMed:24699545, PubMed:25032706, PubMed:25319259, PubMed:29880558).
Restricts the accumulation of lin-29 in the hypodermis to the larval L4 stage, thus controlling terminal differentiation of seam cells (PubMed:10550049, PubMed:21471153).
Has a role in the miRNA-mediated specification of asymmetric gene expression patterns in gustatory neurons (PubMed:25032706).
May also regulate genes involved in other biological processes including transport, small molecule metabolism, and growth (PubMed:25032706).
Inhibits dauer formation, by antagonizing daf-12 (PubMed:20843862).

Isoform a

Specifically required for maintaining the timing of larval development and molting cycle rhythms.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentnucleus
Molecular FunctionRNA polymerase II-specific DNA-binding transcription factor binding
Biological Processcell differentiation
Biological Processnegative regulation of dauer larval development
Biological Processnegative regulation of miRNA transcription
Biological Processregulation of development, heterochronic

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Period protein homolog lin-42
  • Alternative names
    • Abnormal cell lineage protein 42

Gene names

    • Name
      lin-42
    • ORF names
      F47F6.1

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    Q65ZG8
  • Secondary accessions
    • H2L053
    • P91313

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Cytoplasm
Note: Expression is generally more enriched in the nuclei than the cytoplasm (PubMed:10550049).
Cytoplasmic expression is enhanced in lateral seam cells during the molt periods of larval development (PubMed:16300753).

Keywords

Phenotypes & Variants

Disruption phenotype

Mutants exhibit a delay in development characterized by a strong molting defect, resulting in a prolonged L1 stage followed by an arrest in development in the L2 stage in most animals (PubMed:25319259).
There is increased expression of the heterochronic miRNAs, lin-4 and miR-48, in late L1 (PubMed:25319259).
In addition, there is increased expression of the heterochronic miRNA let-7 at the L3 and L4 larval stages (PubMed:29880558).
Seam cell shape and connectivity is severely abnormal during the molt stages (PubMed:22137474).
Animals display a precocious alae phenotype, with the early formation of either full or partial alae in the adult cuticle (PubMed:25319259, PubMed:29880558).
The precocious alae phenotype is suppressed in the double lin-42 and let-7 mutant and lin-42 and miR-48 mutant (PubMed:25319259).
The quadruple lin-42, miR-48, miR-241 and let-7 mutant has a retarded phenotype at L4, with many animals having few full and partial alae or none (PubMed:25319259).
The number of animals displaying a squat body statue, referred to as a dumpy phenotype, is increased, the incomplete alae formation defect is rescued and increased let-7 levels are reduced in a kin-20 RNAi-mediated knockdown mutant background (PubMed:29880558).
RNAi-mediated knockdown leads to a severe hypodermal phenotype, premature execution of developmental events in vulval precursor cells, DTC and sex myoblasts (PubMed:16300753).
Furthermore, seam cell terminal differentiation is only partially initiated at the L4 larval stage (PubMed:15691769).
RNAi-mediated knockdown increases the survival rate and partially restores alae formation of let-7 n2853 mutants at 20 degrees Celsius (PubMed:15691769).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004323851-597Period protein homolog lin-42

Proteomic databases

Expression

Developmental stage

Expressed from late embryonic stage onwards (PubMed:10550049, PubMed:16300753).
Shows oscillating expression levels during larval stages, with peak levels in intermolt periods and minimal levels during ecdysis (PubMed:16300753, PubMed:20843862, PubMed:22137474, PubMed:29880558).
Expressed in hypodermis, vulva, intestine, muscle, neurons and somatic gonad throughout larval development, in an oscillating pattern (PubMed:16300753, PubMed:22137474).
Expressed in sex myoblasts during larval stages L1, L2 and L3, in an oscillating pattern (PubMed:16300753).
Expressed in gonad distal tip cells (DTC) during the L2 and L3 stages, in an oscillating pattern (PubMed:16300753).

Isoform a

Expressed in the pharyngeal myoepithelium, the major body hypodermal syncytium and lateral seam cells from the mid L1 stage. Expression in the hypodermis rises and falls at the start and end of every molt, respectively.

Gene expression databases

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain, compositional bias.

TypeIDPosition(s)Description
Region1-44Disordered
Domain155-223PAS
Region313-335Disordered
Region418-450Disordered
Compositional bias424-441Basic and acidic residues
Region473-509Disordered
Compositional bias483-499Polar residues
Region555-597Disordered
Compositional bias564-597Polar residues

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete

This entry describes 3 isoforms produced by Alternative promoter usage & Alternative splicing. Additional isoforms seem to exist.

Q65ZG8-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    b
  • Synonyms
    c
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    597
  • Mass (Da)
    65,255
  • Last updated
    2004-10-11 v1
  • Checksum
    26ED5D4061A5CA43
MEPAGHSSATHNIVVPNANPTQPQPLAPAMREEGATLSPPNTWSSSSVEFLDDADDNRLLFTCTFTLPHGTVLSSATYADGFHEQYLTIGDNFLARLEPKGQSFILSAAAASVKQRIFARVTMPDGALRACELLCEFETDRAKITVLALRSAFSLQASHVSSNFHVFTFITKHSSTCALTHIDYASIPYLGLLPTDLIGKSLLAFVYSPDVHVVRQAHIDLHNSRGKIVKSIADLRLVAHNGSILRCQTEWSAYVNPWTRKMELVVARHRICSLPIGDSDVISSPPPGIQSNTLPPVMAKTFEDELRTIMNKPVPSTSRHSHHHHHSSLKDQNQGFPANIDLGAYIDKIVEQLVVNSTAQQQQKVAVAAAAAAQAAQAAVVATAQIRKVASAPPTTSTDPPLSYTQINCLENVHRLLKSQSRPESPAKQDEPFDEKKYPPQTPLTREALTLHTKRFEDEYKDTWCRRLKRLSDDVPSSPPAKRTTPIHWTSSSQNHYRTMAPAPPPPPGKNYQITYTPLDDLTDQKSTNTKSDVENVAYPISGSKFSTPMRLSIDGLLPRGATSTGGASPTSGTNSPPVFPKTSSSSSLLMLRDSQN

Q65ZG8-2

  • Name
    a
  • Synonyms
    d
  • Note
    Produced by alternative promoter usage.
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-312: MEPAGHSSATHNIVVPNANPTQPQPLAPAMREEGATLSPPNTWSSSSVEFLDDADDNRLLFTCTFTLPHGTVLSSATYADGFHEQYLTIGDNFLARLEPKGQSFILSAAAASVKQRIFARVTMPDGALRACELLCEFETDRAKITVLALRSAFSLQASHVSSNFHVFTFITKHSSTCALTHIDYASIPYLGLLPTDLIGKSLLAFVYSPDVHVVRQAHIDLHNSRGKIVKSIADLRLVAHNGSILRCQTEWSAYVNPWTRKMELVVARHRICSLPIGDSDVISSPPPGIQSNTLPPVMAKTFEDELRTIMNK → MDILSYLYDLAE

Q65ZG8-3

  • Name
    c
  • Synonyms
    a
  • Note
    Produced by alternative splicing.
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 313-453: PVPSTSRHSHHHHHSSLKDQNQGFPANIDLGAYIDKIVEQLVVNSTAQQQQKVAVAAAAAAQAAQAAVVATAQIRKVASAPPTTSTDPPLSYTQINCLENVHRLLKSQSRPESPAKQDEPFDEKKYPPQTPLTREALTLHT → VSSAISSVTFLSSGDGASHRLSSSSVLLVLTLHFYLCASLRRDTRLARRIRRRESGQQRHLSEQKVHMLALRSMAPCQEKECKNDALAGGAVSGTTDELIPRRTFLEFAWNGECNFIGGPVLYGSRRDMVQVLQKPTKLKS
    • 454-597: Missing

Computationally mapped potential isoform sequences

There are 4 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A131MBE1A0A131MBE1_CAEELlin-42510
A0A131MAY5A0A131MAY5_CAEELlin-42654
A0A131MAT8A0A131MAT8_CAEELlin-42336
A0A131MCB3A0A131MCB3_CAEELlin-4298

Features

Showing features for alternative sequence, compositional bias.

TypeIDPosition(s)Description
Alternative sequenceVSP_0575031-312in isoform a
Alternative sequenceVSP_057504313-453in isoform c
Compositional bias424-441Basic and acidic residues
Alternative sequenceVSP_057505454-597in isoform c
Compositional bias483-499Polar residues
Compositional bias564-597Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF183400
EMBL· GenBank· DDBJ
AAF13188.1
EMBL· GenBank· DDBJ
mRNA
BX284602
EMBL· GenBank· DDBJ
CCD71436.1
EMBL· GenBank· DDBJ
Genomic DNA
BX284602
EMBL· GenBank· DDBJ
CCD71435.1
EMBL· GenBank· DDBJ
Genomic DNA
BX284602
EMBL· GenBank· DDBJ
CCD71437.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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