Q65RN7 · DNLJ_MANSM

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

167550100150200250300350400450500550600650
TypeIDPosition(s)Description
Binding site34-38NAD+ (UniProtKB | ChEBI)
Binding site83-84NAD+ (UniProtKB | ChEBI)
Binding site116NAD+ (UniProtKB | ChEBI)
Active site118N6-AMP-lysine intermediate
Binding site139NAD+ (UniProtKB | ChEBI)
Binding site176NAD+ (UniProtKB | ChEBI)
Binding site293NAD+ (UniProtKB | ChEBI)
Binding site317NAD+ (UniProtKB | ChEBI)
Binding site411Zn2+ (UniProtKB | ChEBI)
Binding site414Zn2+ (UniProtKB | ChEBI)
Binding site429Zn2+ (UniProtKB | ChEBI)
Binding site435Zn2+ (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Molecular FunctionDNA binding
Molecular FunctionDNA ligase (NAD+) activity
Molecular Functionmetal ion binding
Biological ProcessDNA repair
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • Ordered locus names
      MS1766

Organism names

Accessions

  • Primary accession
    Q65RN7

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003133011-675DNA ligase

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain594-675BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    675
  • Mass (Da)
    74,740
  • Last updated
    2004-10-25 v1
  • Checksum
    0E204248ECD1E756
MTIMDINQQIKQLRDTLRYHEYQYHVLDDPKIPDAEYDRLFHQLKALEQQHPELITADSPTQRVGAKPLAGFAQITHELPMLSLDNAFSDEEFNAFVKRIQDRLIVLPQPLTFCCEPKLDGLAVSIFYVNGVLTQAATRGDGTTGEDITLNIRTIRNIPLQLLTDNPPARLEVRGEVFMPHEGFNRLNERALEHGEKTFANPRNAAAGSLRQLDPKITSRRPLVFNAYSVGIAEGVELPATHYERLQWLKSVGIPVNSEVQLCDGSEKVLEFYRSMQQKRPTLGYDIDGTVLKINDIGLQRELGFISKAPRWAIAYKFPAQEELTRLNDVEFQVGRTGAITPVAKLAPVFVAGVTVSNATLHNGDEIARLDIAIGDTVVIRRAGDVIPQIIGVLHERRPANAQAIVFPTQCPVCGSKIVRIEGEAVARCTGGLFCDAQRKEALKHFVSRRAMDIDGVGAKLIEQLVDKELIRTPADLFKLDLITLMRLERMGEKSAQNALDSLEKAKNTTLARFIFALGIREVGEATALNLANHFKNLDALQAASPEQLQEVADVGEVVANRIYVFWREQHNIDAVNDLIAQGIHWETVETKEAGENPFKGKTVVLTGTLTQMGRNETKDLLQQLGAKAAGSVSAKTHFVIAGDNAGSKLTKAQELGVAVMSEAEFLAIVNAYKR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE016827
EMBL· GenBank· DDBJ
AAU38373.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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