Q65RI4 · NRFA_MANSM

Function

function

Catalyzes the reduction of nitrite to ammonia, consuming six electrons in the process.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Ca2+ (UniProtKB | Rhea| CHEBI:29108 )

Note: Binds 1 Ca2+ ion per monomer.
heme c (UniProtKB | Rhea| CHEBI:61717 )

Note: Binds 5 heme c groups covalently per monomer.

Pathway

Nitrogen metabolism; nitrate reduction (assimilation).

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site129Fe (UniProtKB | ChEBI) of heme c 3 (UniProtKB | ChEBI); axial binding residue
Binding site157heme 1 (UniProtKB | ChEBI); covalent
Binding site160heme 1 (UniProtKB | ChEBI); covalent
Binding site161Fe (UniProtKB | ChEBI) of heme 1 (UniProtKB | ChEBI); axial binding residue
Binding site195heme c 2 (UniProtKB | ChEBI); covalent
Binding site198heme c 2 (UniProtKB | ChEBI); covalent
Binding site199Fe (UniProtKB | ChEBI) of heme c 2 (UniProtKB | ChEBI); axial binding residue
Binding site244heme c 3 (UniProtKB | ChEBI); covalent
Binding site247heme c 3 (UniProtKB | ChEBI); covalent
Binding site248Fe (UniProtKB | ChEBI) of heme c 3 (UniProtKB | ChEBI); axial binding residue
Binding site250Ca2+ (UniProtKB | ChEBI)
Binding site251Ca2+ (UniProtKB | ChEBI)
Binding site251substrate
Binding site298Ca2+ (UniProtKB | ChEBI)
Binding site300Ca2+ (UniProtKB | ChEBI)
Binding site301substrate
Binding site312Fe (UniProtKB | ChEBI) of heme c 5 (UniProtKB | ChEBI); axial binding residue
Binding site319heme c 4 (UniProtKB | ChEBI); covalent
Binding site322heme c 4 (UniProtKB | ChEBI); covalent
Binding site323Fe (UniProtKB | ChEBI) of heme c 4 (UniProtKB | ChEBI); axial binding residue
Binding site338Fe (UniProtKB | ChEBI) of heme c 2 (UniProtKB | ChEBI); axial binding residue
Binding site353heme c 5 (UniProtKB | ChEBI); covalent
Binding site356heme c 5 (UniProtKB | ChEBI); covalent
Binding site357Fe (UniProtKB | ChEBI) of heme c 5 (UniProtKB | ChEBI); axial binding residue
Binding site432Fe (UniProtKB | ChEBI) of heme c 4 (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentperiplasmic space
Molecular Functioncalcium ion binding
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionnitrite reductase (cytochrome, ammonia-forming) activity
Biological Processnitrate assimilation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytochrome c-552
  • EC number
  • Alternative names
    • Ammonia-forming cytochrome c nitrite reductase
      (Cytochrome c nitrite reductase
      )

Gene names

    • Name
      nrfA
    • Ordered locus names
      MS1819

Organism names

Accessions

  • Primary accession
    Q65RI4

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-32
ChainPRO_000026896833-525Cytochrome c-552

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region43-63Disordered

Sequence similarities

Belongs to the cytochrome c-552 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    525
  • Mass (Da)
    58,466
  • Last updated
    2004-10-25 v1
  • Checksum
    747EF08AFB3036BB
MNGVIIVNILRKTLSSLAIVGLGFAMANSAVAEEKAMATHQQAQQLQQPAPETAAKRAPTKEELTPVNPNLKIEAANEKFAADFPRQYNSWAKTAEQTEFHKEVEDDPRMIVMWGGYAFAKEFNSPRGHIYAVTDVRNILRTGSPKDANGGPQPMACWTCKGPDVPRLIAEWGEEGYFSGKWAKGGAEVVNSIGCADCHDTQSQDFKDGKPALRVARPHVLRALDTVGKTFATSDRTDQRAGVCANCHVEYYFDKSTGANNVVFPWYKGRDVDSIEKYYDEIGFKDWEHSISKAPMLKAQHPDFETWSMGTHGKNGVTCVDCHMAKTQDKDGKVYTDHQVVGNPVKDNFQNTCARCHDQSQDTLIKTVEQHKADVREVMLKLEDQLVKSHFEAKTAWDNGATQEEMKDALQAIRHAQWRWDFAAASHGMHMHAPDVALKIIASGLDRVADARAKLAVILAKHGVQQPIQYPDISTAEKAWKVMGIDIEKERKEKEEFIKTVIPEWNKEAISKGLILTAPPTTPAK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE016827
EMBL· GenBank· DDBJ
AAU38426.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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