Q65JS3 · RLMN_BACLD

Function

function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site104Proton acceptor
Binding site124[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site128[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site131[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site174-175S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site206S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site229-231S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site305S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site348S-methylcysteine intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctionrRNA (adenine(2503)-C2-)-methyltransferase activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA (adenine(37)-C2)-methyltransferase activity
Molecular FunctiontRNA binding
Biological ProcessrRNA base methylation
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Probable dual-specificity RNA methyltransferase RlmN
  • EC number
  • Alternative names
    • 23S rRNA (adenine(2503)-C(2))-methyltransferase
    • 23S rRNA m2A2503 methyltransferase
    • Ribosomal RNA large subunit methyltransferase N
    • tRNA (adenine(37)-C(2))-methyltransferase
    • tRNA m2A37 methyltransferase

Gene names

    • Name
      rlmN
    • Ordered locus names
      BLi01796, BL02300

Organism names

Accessions

  • Primary accession
    Q65JS3
  • Secondary accessions
    • Q62V78

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00003500351-361Probable dual-specificity RNA methyltransferase RlmN
Disulfide bond117↔348(transient)

Keywords

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain110-343Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    361
  • Mass (Da)
    41,386
  • Last updated
    2004-10-25 v1
  • Checksum
    96E1CF18B26CB9AC
MESKQKVRKELRTERPSIYSFEIEELKSWLSENGEKPFRAAQIFEWLYEKRVTSFEKMTNLSKELRTKLNEHFVLTTLKTAVKQTSQDGTMKFLFELHDGYTIETVLMRHEYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVKVQQALDETDERVSSIVIMGIGEPFDNFQEMLAFLKIVNHDKGLNIGARHITVSTSGIIPKIYEFADEKLQINFAISLHAPNTEIRSRLMPINRAYKLPDLMKAVDYYIKKTGRRVTFEYGLFGGVNDQVEHAEELAELLKGIKCHVNLIPVNYVPERDYVRTPKEQIFAFEKTLKSHGVNVTIRREQGHDIDAACGQLRAKERQEETR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000002
EMBL· GenBank· DDBJ
AAU23331.1
EMBL· GenBank· DDBJ
Genomic DNA
AE017333
EMBL· GenBank· DDBJ
AAU40691.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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