Q65949 · CAP3_ADECC
- ProteinPre-hexon-linking protein IIIa
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids563 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Structural component of the virion that acts as a cement protein on the capsid exterior which mediates the interactions between the hexons, including the peripentonal hexons, and reaches all the way to the penton vertices. Two hexon linking proteins IIIa, one from each facet, stabilize the unique edge interface between a pair of facets. As the virus enters the host cell, hexon linking proteins IIIa are shed concomitant with virion acidification in the endosome. During virus assembly, seems to play a role in the serotype specificity of the packaging of viral DNA via its interaction with packaging protein 3.
Miscellaneous
All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 548-549 | Cleavage; by viral protease | ||||
Sequence: SG |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell nucleus | |
Cellular Component | viral capsid, decoration |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended namePre-hexon-linking protein IIIa
- Alternative names
- Cleaved into 1 chains
Gene names
Organism names
- Taxonomic lineageViruses > Varidnaviria > Bamfordvirae > Preplasmiviricota > Tectiliviricetes > Rowavirales > Adenoviridae > Mastadenovirus > Canine mastadenovirus A
- Virus hosts
Accessions
- Primary accessionQ65949
Subcellular Location
UniProt Annotation
GO Annotation
Note: Surrounds the border of each facet on the capsid exterior. Present in around 60 copies per virion.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue, propeptide.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000421390 | 1-548 | Hexon-linking protein IIIa | |||
Sequence: MRKRRTLTAPSHFNSMSAALNPMKMAAMQSQPTVDDSWAASISRIMSLTAGDRHKFSSQPFANRLDAILEAVVPSRKDPTHEKVLTIVNALIENGAIRRDEGAGVYDALLHRVSKYNSINTQSNLERLAGDVREAVAQQVRIAAGNLGSLTALNSFLARLPANVERGQDNYTGFLSALKLLVSEVPNTEVYQSGPHYFLQSSRNGTQTVNLTNAFENLKPLWGVKAPTMERLSISALLTPNTRLLLLLVSPFTDSVSISRDSYLGYLLTLYREALGRNHLDERTLEEVTEVSKAMGNENINNLQATLNFLLTNRQKKIPKDYSLTPEEERIVRFIQQAVSLRMMQENLSPTEALDVTAANMEPSFYANNRDFINKLMDYFHRAAAIAPDYFLGAVMNPRWLPPEGFFTGVFDFPERDNYIWDGPDSSLDLTRQDAMRFLEEKFIDDDQRTESRSVSRVPTPASSRRSSVAMASDSLIRPMNNDKNSLREIEVLADKLARWKTYKRESEEARESLPVVVRPKKYSSAISSDESDDGMSKPDKFLKFEGS | ||||||
Chain | PRO_0000221837 | 1-563 | Pre-hexon-linking protein IIIa | |||
Sequence: MRKRRTLTAPSHFNSMSAALNPMKMAAMQSQPTVDDSWAASISRIMSLTAGDRHKFSSQPFANRLDAILEAVVPSRKDPTHEKVLTIVNALIENGAIRRDEGAGVYDALLHRVSKYNSINTQSNLERLAGDVREAVAQQVRIAAGNLGSLTALNSFLARLPANVERGQDNYTGFLSALKLLVSEVPNTEVYQSGPHYFLQSSRNGTQTVNLTNAFENLKPLWGVKAPTMERLSISALLTPNTRLLLLLVSPFTDSVSISRDSYLGYLLTLYREALGRNHLDERTLEEVTEVSKAMGNENINNLQATLNFLLTNRQKKIPKDYSLTPEEERIVRFIQQAVSLRMMQENLSPTEALDVTAANMEPSFYANNRDFINKLMDYFHRAAAIAPDYFLGAVMNPRWLPPEGFFTGVFDFPERDNYIWDGPDSSLDLTRQDAMRFLEEKFIDDDQRTESRSVSRVPTPASSRRSSVAMASDSLIRPMNNDKNSLREIEVLADKLARWKTYKRESEEARESLPVVVRPKKYSSAISSDESDDGMSKPDKFLKFEGSGNPFAHLRPKLGRCL | ||||||
Modified residue | 235 | Phosphoserine; by host | ||||
Sequence: S | ||||||
Modified residue | 284 | Phosphothreonine; by host | ||||
Sequence: T | ||||||
Modified residue | 452 | Phosphoserine; by host | ||||
Sequence: S | ||||||
Modified residue | 456 | Phosphoserine; by host | ||||
Sequence: S | ||||||
Modified residue | 475 | Phosphoserine; by host | ||||
Sequence: S | ||||||
Modified residue | 486 | Phosphoserine; by host | ||||
Sequence: S | ||||||
Propeptide | PRO_0000421389 | 549-563 | ||||
Sequence: GNPFAHLRPKLGRCL |
Post-translational modification
Cleaved near the C-terminus by the viral protease during virion maturation to form the mature protein.
Keywords
- PTM
Expression
Induction
Expressed in the late phase of the viral replicative cycle.
Keywords
- Developmental stage
Interaction
Subunit
Interacts with hexon proteins; this interaction tethers the peripentonal hexons to hexons situated in the facet. Interacts with the penton protein (via N-terminus). Interacts with packaging protein 3; this interaction is required to promote correct genome packaging.
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-117 | Peripentonal hexon-tethering domain | ||||
Sequence: MRKRRTLTAPSHFNSMSAALNPMKMAAMQSQPTVDDSWAASISRIMSLTAGDRHKFSSQPFANRLDAILEAVVPSRKDPTHEKVLTIVNALIENGAIRRDEGAGVYDALLHRVSKYN | ||||||
Region | 148-261 | Binding to hexon-linking protein | ||||
Sequence: GSLTALNSFLARLPANVERGQDNYTGFLSALKLLVSEVPNTEVYQSGPHYFLQSSRNGTQTVNLTNAFENLKPLWGVKAPTMERLSISALLTPNTRLLLLLVSPFTDSVSISRD | ||||||
Region | 449-472 | Disordered | ||||
Sequence: RTESRSVSRVPTPASSRRSSVAMA | ||||||
Compositional bias | 454-472 | Polar residues | ||||
Sequence: SVSRVPTPASSRRSSVAMA | ||||||
Region | 522-543 | Disordered | ||||
Sequence: KYSSAISSDESDDGMSKPDKFL |
Sequence similarities
Belongs to the adenoviridae hexon-linking protein IIIa family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length563
- Mass (Da)63,283
- Last updated1997-11-01 v1
- Checksum4681E319AC6AFA63
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 454-472 | Polar residues | ||||
Sequence: SVSRVPTPASSRRSSVAMA |