Q65947 · TERM_ADECC
- ProteinPreterminal protein
- GenePTP
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids608 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Protein covalently bound to the viral DNA that acts as a primer for viral genomic replication by DNA strand displacement. Assembles on the viral origin of replication in an initiation complex with viral polymerase, DBP, host NFIA and host POU2F1/OCT1. During initiation, the polymerase covalently couples the first dCTP with Ser-580 of pTP. The terminal protein stimulates the template activity over 20 fold compared to protein-free templates. Neo-synthesized viral genomes are linked to two preterminal proteins, one for each 5' end. These new genomes are encapsidated in the nucleus, and during capsid maturation by viral protease, preterminal protein is first cleaved into intermediary (iTP), then into mature TP. May play a role in host nuclear matrix localization of genomic DNA.
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 175-176 | Cleavage; by adenovirus protease | ||||
Sequence: YG | ||||||
Site | 307-308 | Cleavage; by adenovirus protease | ||||
Sequence: GA | ||||||
Site | 536 | Priming of strand displacement replication by covalently linking the first nucleotide of the new DNA chain | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell nuclear matrix | |
Molecular Function | double-stranded DNA binding | |
Molecular Function | single-stranded DNA binding | |
Biological Process | DNA replication | |
Biological Process | viral DNA strand displacement replication |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended namePreterminal protein
- Short namespTP
- Alternative names
- Cleaved into 2 chains
Gene names
Organism names
- Taxonomic lineageViruses > Varidnaviria > Bamfordvirae > Preplasmiviricota > Tectiliviricetes > Rowavirales > Adenoviridae > Mastadenovirus > Canine mastadenovirus A
- Virus hosts
Accessions
- Primary accessionQ65947
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000221900 | 1-608 | Preterminal protein | |||
Sequence: MSLNALDCARLTGQTPYTVEVFRPIRNIFNRVREYTRASTTSVGLAWMSKYIYQYHRLMLMNLSPREPATEGWPLFLYPPPHLLVGYQYLVRTCNDYVFDTRSYSRLKYTEIHLPLQQKLNWTVMANCSYTINTGAYHRFIDFENFEETLAQVQQAVLAERVVADLALIRPMRGYGTTNMAGDRQVPVEGLLQDHYKNLSQCQNHAWGLADRMRIQNAGNKDIVILTTIRKLKTAFFNFLVSPRNPHTILSLPCDCLWLDAFMQKFTDPSLSQFQTIQSLPSQSVTKSIISALSLPGPAPCTPLSGGAFELRPRENGRAVTEEMRRRRGEMIERFIDRLPMRRRRRRAPPPPPMSEELSEPEVEAFPPASPPRRSFEEEVRDTIVEAIRLLQEELTVSARNEQFFNFAINFYEVIQRLEMLGNINELTIRRWVMYFFVAEHVATTLNYLHHNLRLYPPCSRWVDLELAQVVMRARDHEGQVVYSRVWNEMGENAFSQLMARVSGDLAATVERAGLGELEEEEMEQFMADIAYHENSGDVSEILRQVAINDTEVDSMELSFRFKVTGPVVFSQNRQIQSINRRVVALASQLRMQHRPLPAQHEQVQLPP | ||||||
Chain | PRO_0000433944 | 176-608 | Intermediate terminal protein | |||
Sequence: GTTNMAGDRQVPVEGLLQDHYKNLSQCQNHAWGLADRMRIQNAGNKDIVILTTIRKLKTAFFNFLVSPRNPHTILSLPCDCLWLDAFMQKFTDPSLSQFQTIQSLPSQSVTKSIISALSLPGPAPCTPLSGGAFELRPRENGRAVTEEMRRRRGEMIERFIDRLPMRRRRRRAPPPPPMSEELSEPEVEAFPPASPPRRSFEEEVRDTIVEAIRLLQEELTVSARNEQFFNFAINFYEVIQRLEMLGNINELTIRRWVMYFFVAEHVATTLNYLHHNLRLYPPCSRWVDLELAQVVMRARDHEGQVVYSRVWNEMGENAFSQLMARVSGDLAATVERAGLGELEEEEMEQFMADIAYHENSGDVSEILRQVAINDTEVDSMELSFRFKVTGPVVFSQNRQIQSINRRVVALASQLRMQHRPLPAQHEQVQLPP | ||||||
Chain | PRO_0000433945 | 308-608 | Terminal protein | |||
Sequence: AFELRPRENGRAVTEEMRRRRGEMIERFIDRLPMRRRRRRAPPPPPMSEELSEPEVEAFPPASPPRRSFEEEVRDTIVEAIRLLQEELTVSARNEQFFNFAINFYEVIQRLEMLGNINELTIRRWVMYFFVAEHVATTLNYLHHNLRLYPPCSRWVDLELAQVVMRARDHEGQVVYSRVWNEMGENAFSQLMARVSGDLAATVERAGLGELEEEEMEQFMADIAYHENSGDVSEILRQVAINDTEVDSMELSFRFKVTGPVVFSQNRQIQSINRRVVALASQLRMQHRPLPAQHEQVQLPP | ||||||
Modified residue | 536 | O-(5'-phospho-DNA)-serine | ||||
Sequence: S |
Post-translational modification
Preterminal protein is used to replicate viral genome, upon genomic encapsidation it is processed first into iTP and finally into TP by adenovirus protease.
Keywords
- PTM
Interaction
Subunit
Heterodimer with the polymerase; this heterodimer binds to bp 9 to 18 of the genome. Interacts with host POU2F1; POU2F1 binds to the auxiliary sequences in the inverted terminal repeats and tethers the pTP-POL heterodimer to the origin DNA thereby participating in the assembly of the pre-initiation complex (POL-TP-DBP-NFIA-POU2F1).
Structure
Family & Domains
Features
Showing features for motif, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 338-347 | Nuclear localization signal | ||||
Sequence: RLPMRRRRRR | ||||||
Region | 342-377 | Disordered | ||||
Sequence: RRRRRRAPPPPPMSEELSEPEVEAFPPASPPRRSFE |
Sequence similarities
Belongs to the adenoviridae terminal protein family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length608
- Mass (Da)70,283
- Last updated1997-11-01 v1
- Checksum328AEA9602919848