Q65947 · TERM_ADECC

Function

function

Protein covalently bound to the viral DNA that acts as a primer for viral genomic replication by DNA strand displacement. Assembles on the viral origin of replication in an initiation complex with viral polymerase, DBP, host NFIA and host POU2F1/OCT1. During initiation, the polymerase covalently couples the first dCTP with Ser-580 of pTP. The terminal protein stimulates the template activity over 20 fold compared to protein-free templates. Neo-synthesized viral genomes are linked to two preterminal proteins, one for each 5' end. These new genomes are encapsidated in the nucleus, and during capsid maturation by viral protease, preterminal protein is first cleaved into intermediary (iTP), then into mature TP. May play a role in host nuclear matrix localization of genomic DNA.

Features

Showing features for site.

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TypeIDPosition(s)Description
Site175-176Cleavage; by adenovirus protease
Site307-308Cleavage; by adenovirus protease
Site536Priming of strand displacement replication by covalently linking the first nucleotide of the new DNA chain

GO annotations

AspectTerm
Cellular Componenthost cell nuclear matrix
Molecular Functiondouble-stranded DNA binding
Molecular Functionsingle-stranded DNA binding
Biological ProcessDNA replication
Biological Processviral DNA strand displacement replication

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Preterminal protein
  • Short names
    pTP
  • Alternative names
    • Bellett protein
    • Precursor terminal protein
  • Cleaved into 2 chains

Gene names

    • Name
      PTP

Organism names

Accessions

  • Primary accession
    Q65947

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00002219001-608Preterminal protein
ChainPRO_0000433944176-608Intermediate terminal protein
ChainPRO_0000433945308-608Terminal protein
Modified residue536O-(5'-phospho-DNA)-serine

Post-translational modification

Preterminal protein is used to replicate viral genome, upon genomic encapsidation it is processed first into iTP and finally into TP by adenovirus protease.

Keywords

Interaction

Subunit

Heterodimer with the polymerase; this heterodimer binds to bp 9 to 18 of the genome. Interacts with host POU2F1; POU2F1 binds to the auxiliary sequences in the inverted terminal repeats and tethers the pTP-POL heterodimer to the origin DNA thereby participating in the assembly of the pre-initiation complex (POL-TP-DBP-NFIA-POU2F1).

Family & Domains

Features

Showing features for motif, region.

TypeIDPosition(s)Description
Motif338-347Nuclear localization signal
Region342-377Disordered

Sequence similarities

Belongs to the adenoviridae terminal protein family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    608
  • Mass (Da)
    70,283
  • Last updated
    1997-11-01 v1
  • Checksum
    328AEA9602919848
MSLNALDCARLTGQTPYTVEVFRPIRNIFNRVREYTRASTTSVGLAWMSKYIYQYHRLMLMNLSPREPATEGWPLFLYPPPHLLVGYQYLVRTCNDYVFDTRSYSRLKYTEIHLPLQQKLNWTVMANCSYTINTGAYHRFIDFENFEETLAQVQQAVLAERVVADLALIRPMRGYGTTNMAGDRQVPVEGLLQDHYKNLSQCQNHAWGLADRMRIQNAGNKDIVILTTIRKLKTAFFNFLVSPRNPHTILSLPCDCLWLDAFMQKFTDPSLSQFQTIQSLPSQSVTKSIISALSLPGPAPCTPLSGGAFELRPRENGRAVTEEMRRRRGEMIERFIDRLPMRRRRRRAPPPPPMSEELSEPEVEAFPPASPPRRSFEEEVRDTIVEAIRLLQEELTVSARNEQFFNFAINFYEVIQRLEMLGNINELTIRRWVMYFFVAEHVATTLNYLHHNLRLYPPCSRWVDLELAQVVMRARDHEGQVVYSRVWNEMGENAFSQLMARVSGDLAATVERAGLGELEEEEMEQFMADIAYHENSGDVSEILRQVAINDTEVDSMELSFRFKVTGPVVFSQNRQIQSINRRVVALASQLRMQHRPLPAQHEQVQLPP

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U55001
EMBL· GenBank· DDBJ
AAB05435.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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