Q64687 · SIA8A_MOUSE

  • Protein
    Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
  • Gene
    St8sia1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Catalyzes the addition of sialic acid in alpha 2,8-linkage to the sialic acid moiety of the ganglioside GM3 to form ganglioside GD3; gangliosides are a subfamily of complex glycosphingolipds that contain one or more residues of sialic acid (PubMed:8910600).
Can catalyze the addition of a second alpha-2,8- sialic acid to GD3 to form GT3 (PubMed:8910600).
Can use GM1b, GD1a and GT1b as acceptor substrates to synthesize GD1c, GT1a and GQ1b respectively (PubMed:8910600).

Catalytic activity

  • an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl derivative + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2->8)-N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl derivative + CMP + H+
    EC:2.4.3.8 (UniProtKB | ENZYME | Rhea)
  • a ganglioside GM3 (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GD3 (d18:1(4E)) + CMP + H+
    This reaction proceeds in the forward direction.
  • a ganglioside GD3 (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GT3 (d18:1(4E)) + CMP + H+
    This reaction proceeds in the forward direction.
  • a ganglioside GD1a (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GT1a (d18:1(4E)) + CMP + H+
    This reaction proceeds in the forward direction.
  • a ganglioside GT1b (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GQ1b (d18:1(4E)) + CMP + H+
    This reaction proceeds in the forward direction.
  • a ganglioside GM1b (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GD1c (d18:1(4E)) + CMP + H+
    This reaction proceeds in the forward direction.
  • a ganglioside GD3 + CMP-N-acetyl-beta-neuraminate = a ganglioside GT3 + CMP + H+
    This reaction proceeds in the forward direction.
  • [alpha-N-acetylneuraminyl-(2->8)](n)-alpha-N-acetylneuraminyl-(2->8)-alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide + CMP-N-acetyl-beta-neuraminate = [alpha-N-acetylneuraminyl-(2->8)](n+1)-alpha-N-acetylneuraminyl-(2->8)-alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide + CMP + H+
    This reaction proceeds in the forward direction.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.03 mMganglioside GM3
5 mMganglioside GD3
5 mMganglioside GD1a
2 mMganglioside GT1b

Pathway

Protein modification; protein glycosylation.
Lipid metabolism; sphingolipid metabolism.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site142CMP-N-acetyl-beta-neuraminate (UniProtKB | ChEBI)
Binding site165CMP-N-acetyl-beta-neuraminate (UniProtKB | ChEBI)
Binding site273CMP-N-acetyl-beta-neuraminate (UniProtKB | ChEBI)
Binding site274CMP-N-acetyl-beta-neuraminate (UniProtKB | ChEBI)
Binding site275CMP-N-acetyl-beta-neuraminate (UniProtKB | ChEBI)
Binding site295CMP-N-acetyl-beta-neuraminate (UniProtKB | ChEBI)
Binding site309CMP-N-acetyl-beta-neuraminate (UniProtKB | ChEBI)
Active site321Proton donor/acceptor

GO annotations

AspectTerm
Cellular ComponentGolgi membrane
Molecular Functionalpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity
Biological Processcell population proliferation
Biological Processcellular response to heat
Biological Processepithelial cell proliferation
Biological Processpositive regulation of epithelial cell proliferation
Biological Processprotein glycosylation
Biological Processsphingolipid metabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
  • EC number
  • Alternative names
    • Alpha-2,8-sialyltransferase 8A
    • Ganglioside GD3 synthase
    • Ganglioside GT3 synthase
    • Sialyltransferase 8A (SIAT8-A)
    • Sialyltransferase St8Sia I (ST8SiaI)

Gene names

    • Name
      St8sia1
    • Synonyms
      Siat8, Siat8a

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q64687
  • Secondary accessions
    • Q8K1C1

Proteomes

Organism-specific databases

Subcellular Location

Golgi apparatus membrane
; Single-pass type II membrane protein

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-28Cytoplasmic
Transmembrane29-47Helical; Signal-anchor for type II membrane protein
Topological domain48-355Lumenal

Keywords

PTM/Processing

Features

Showing features for chain, glycosylation, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00001492831-355Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
Glycosylation70N-linked (GlcNAc...) asparagine
Glycosylation118N-linked (GlcNAc...) asparagine
Disulfide bond137↔286
Disulfide bond151↔346
Glycosylation213N-linked (GlcNAc...) asparagine
Glycosylation244N-linked (GlcNAc...) asparagine

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Sequence similarities

Belongs to the glycosyltransferase 29 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    355
  • Mass (Da)
    40,324
  • Last updated
    2011-07-27 v2
  • Checksum
    220BD56BBEE6E6C6
MSPCGRALHTSRGAMAMLARKFPRTRLPVGASALCVVVLCWLYIFPVYRLPNEKEIVQGVLAQRTAWRTNQTSASLFRRQMEDCCDPAHLFAMTKMNSPMGKSLWYDGELLYSFTIDNSTYSLFPQATPFQLPLKKCAVVGNGGILKMSGCGRQIDEANFVMRCNLPPLSSEYTRDVGSKTQLVTANPSIIRQRFENLLWSRKKFVDNMKIYNHSYIYMPAFSMKTGTEPSLRVYYTLKDVGANQTVLFANPNFLRNIGKFWKSRGIHAKRLSTGLFLVSAALGLCEEVSIYGFWPFSVNMQGDPISHHYYDNVLPFSGYHAMPEEFLQLWYLHKIGALRMQLDPCEEPSPQPTS

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0N4SVL1A0A0N4SVL1_MOUSESt8sia1101

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict50in Ref. 1; CAA59014
Sequence conflict64in Ref. 1; CAA59014
Sequence conflict294in Ref. 1; CAA59014
Sequence conflict318in Ref. 1; CAA59014

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X84235
EMBL· GenBank· DDBJ
CAA59014.1
EMBL· GenBank· DDBJ
mRNA
CH466572
EMBL· GenBank· DDBJ
EDL10653.1
EMBL· GenBank· DDBJ
Genomic DNA
BC024821
EMBL· GenBank· DDBJ
AAH24821.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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