Q64685 · SIAT1_MOUSE
- ProteinBeta-galactoside alpha-2,6-sialyltransferase 1
- GeneSt6gal1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids403 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.
Catalytic activity
- a beta-D-galactoside + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2->6)-beta-D-galactosyl derivative + CMP + H+
Pathway
Protein modification; protein glycosylation.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 186 | substrate | |||
Binding site | 209 | substrate | |||
Binding site | 230 | substrate | |||
Binding site | 319-321 | substrate | |||
Binding site | 350 | substrate | |||
Binding site | 351 | substrate | |||
Binding site | 362 | substrate | |||
Binding site | 366 | substrate | |||
Binding site | 367 | substrate | |||
Binding site | 373 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Cellular Component | Golgi cisterna membrane | |
Cellular Component | Golgi medial cisterna | |
Cellular Component | Golgi membrane | |
Cellular Component | Golgi trans cisterna | |
Molecular Function | beta-galactoside alpha-2,6-sialyltransferase activity | |
Molecular Function | protein homodimerization activity | |
Molecular Function | sialyltransferase activity | |
Biological Process | N-acetylneuraminate metabolic process | |
Biological Process | negative regulation of chemotaxis | |
Biological Process | negative regulation of macrophage apoptotic process | |
Biological Process | positive regulation of mononuclear cell proliferation | |
Biological Process | protein glycosylation | |
Biological Process | protein N-linked glycosylation via asparagine | |
Biological Process | regulation of substrate adhesion-dependent cell spreading | |
Biological Process | response to ethanol | |
Biological Process | sialylation |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameBeta-galactoside alpha-2,6-sialyltransferase 1
- EC number
- Short namesAlpha 2,6-ST 1
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ64685
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein
Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Topological domain | 1-9 | Cytoplasmic | |||
Transmembrane | 10-26 | Helical; Signal-anchor for type II membrane protein | |||
Topological domain | 27-403 | Lumenal | |||
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 139 | No effect on enzyme activity. | |||
Mutagenesis | 181 | Loss of enzyme activity. | |||
Mutagenesis | 350 | Loss of enzyme activity. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 31 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, disulfide bond, glycosylation, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000149250 | 1-403 | Beta-galactoside alpha-2,6-sialyltransferase 1 | ||
Disulfide bond | 139↔403 | ||||
Glycosylation | 146 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 158 | N-linked (GlcNAc...) asparagine | |||
Disulfide bond | 181↔332 | ||||
Disulfide bond | 350↔361 | ||||
Modified residue | 366 | Phosphotyrosine | |||
Post-translational modification
The soluble form derives from the membrane form by proteolytic processing.
N-glycosylated.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Length403
- Mass (Da)46,586
- Last updated2011-07-27 v2
- MD5 ChecksumE458CE9AB04EE1AB9615F48BA0895736
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
D3Z1Z5 | D3Z1Z5_MOUSE | St6gal1 | 33 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 131 | in Ref. 1; BAA03680 | |||
Sequence conflict | 136 | in Ref. 1; BAA03680 | |||
Sequence conflict | 155 | in Ref. 1; BAA03680 | |||
Sequence conflict | 170 | in Ref. 1; BAA03680 | |||
Sequence conflict | 178-179 | in Ref. 1; BAA03680 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D16106 EMBL· GenBank· DDBJ | BAA03680.1 EMBL· GenBank· DDBJ | mRNA | ||
AK084124 EMBL· GenBank· DDBJ | BAC39120.1 EMBL· GenBank· DDBJ | mRNA | ||
CH466521 EMBL· GenBank· DDBJ | EDK97666.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH466521 EMBL· GenBank· DDBJ | EDK97667.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC027833 EMBL· GenBank· DDBJ | AAH27833.1 EMBL· GenBank· DDBJ | mRNA | ||
BC092222 EMBL· GenBank· DDBJ | AAH92222.1 EMBL· GenBank· DDBJ | mRNA | ||
BC096026 EMBL· GenBank· DDBJ | AAH96026.1 EMBL· GenBank· DDBJ | mRNA |