Q64442 · DHSO_MOUSE

  • Protein
    Sorbitol dehydrogenase
  • Gene
    Sord
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Polyol dehydrogenase that catalyzes the reversible NAD+-dependent oxidation of various sugar alcohols (By similarity).
Is active with D-sorbitol (D-glucitol) leading to the C2-oxidized product D-fructose (PubMed:6852349).
Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism (By similarity).
May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility and protein tyrosine phosphorylation (PubMed:18799757).
Has no activity on ethanol. Cannot use NADP+ as the electron acceptor (PubMed:6852349).

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Activity regulation

Inhibited in vitro by p-hydroxymercuribenzoate, EDTA, l,l0-phenanthroline and N-ethylmaleimide.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
1.54 mMsorbitol
58.8 μMNAD+
154 mMfructose
15 μMNADH

pH Dependence

Optimum pH is 9.0 for sorbitol oxidation and 7.5 for D-fructose reduction.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site45Zn2+ (UniProtKB | ChEBI); catalytic
Binding site51substrate
Binding site70Zn2+ (UniProtKB | ChEBI); catalytic
Binding site71Zn2+ (UniProtKB | ChEBI); catalytic
Binding site156substrate
Binding site184NAD+ (UniProtKB | ChEBI)
Binding site204NAD+ (UniProtKB | ChEBI)
Binding site209NAD+ (UniProtKB | ChEBI)
Binding site273-275NAD+ (UniProtKB | ChEBI)
Binding site297-299NAD+ (UniProtKB | ChEBI)
Binding site299substrate
Binding site300substrate

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentextracellular exosome
Cellular Componentmitochondrial membrane
Cellular Componentmitochondrion
Cellular Componentmotile cilium
Molecular FunctionD-sorbitol dehydrogenase (acceptor) activity
Molecular FunctionD-xylulose reductase activity
Molecular Functionidentical protein binding
Molecular FunctionL-iditol 2-dehydrogenase (NAD+) activity
Molecular FunctionNAD binding
Molecular Functionzinc ion binding
Biological Processflagellated sperm motility
Biological Processfructose biosynthetic process
Biological Processglucuronate catabolic process to xylulose 5-phosphate
Biological ProcessL-xylitol catabolic process
Biological Processresponse to cadmium ion
Biological Processresponse to copper ion
Biological Processresponse to hormone
Biological Processsorbitol catabolic process
Biological Processsorbitol metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Sorbitol dehydrogenase
  • EC number
  • Short names
    SDH; SORD
  • Alternative names
    • L-iditol 2-dehydrogenase (EC:1.1.1.14
      ) . EC:1.1.1.14 (UniProtKB | ENZYME | Rhea)
    • Polyol dehydrogenase
    • Xylitol dehydrogenase (XDH) (EC:1.1.1.9
      ) . EC:1.1.1.9 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      Sord
    • Synonyms
      Sdh1

Organism names

  • Taxonomic identifier
  • Strains
    • BALB/cJ
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q64442
  • Secondary accessions
    • Q569V5
    • Q9CPS0

Proteomes

Organism-specific databases

Subcellular Location

Mitochondrion membrane
; Peripheral membrane protein
Note: Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface.

Keywords

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylalanine
ChainPRO_00000008822-357Sorbitol dehydrogenase
Modified residue169Phosphoserine

Keywords

Proteomic databases

2D gel databases

PTM databases

Expression

Tissue specificity

Testis has the highest level of expression, followed by kidney, liver, and lung. Low levels of expression are also observed in lens, brain, and skeletal muscle. Expressed in sperm flagellum and very low expression in the sperm head.

Developmental stage

Detected early in spermatogenesis. Detected in condensing spermatids (at protein level) and is up-regulated during late spermatogenesis.

Gene expression databases

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    357
  • Mass (Da)
    38,249
  • Last updated
    2007-07-10 v3
  • Checksum
    FDAA462EF1EB6C21
MAAPAKGENLSLVVHGPGDIRLENYPIPELGPNDVLLKMHSVGICGSDVHYWEHGRIGDFVVKKPMVLGHEAAGTVTKVGELVKHLKPGDRVAIEPGVPREVDEYCKIGRYNLTPTIFFCATPPDDGNLCRFYKHNADFCYKLPDSVTFEEGALIEPLSVGIYACRRGSVSLGNKVLVCGAGPVGMVTLLVAKAMGAAQVVVTDLSASRLTKAKEVGADFTIQVGKETPQEIASKVESLLGSKPEVTIECTGAESSVQTGIYATHSGGTLVIVGMGAEMVNLPLVHAAIREVDIKGVFRYCNTWPMAISMLASKTLNVKPLVTHRFPLEKAVEAFETAKKGVGLKVMIKCDPNDQNP

Sequence caution

The sequence AAA79043.1 differs from that shown. Reason: Erroneous initiation Extended N-terminus.

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict49in Ref. 1; AAA79043
Sequence conflict259in Ref. 1; AAA79043

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U27014
EMBL· GenBank· DDBJ
AAA79043.1
EMBL· GenBank· DDBJ
mRNA Different initiation
AK004692
EMBL· GenBank· DDBJ
BAB23478.1
EMBL· GenBank· DDBJ
mRNA
AK015059
EMBL· GenBank· DDBJ
BAB29695.1
EMBL· GenBank· DDBJ
mRNA
AK166988
EMBL· GenBank· DDBJ
BAE39168.1
EMBL· GenBank· DDBJ
mRNA
AK166996
EMBL· GenBank· DDBJ
BAE39175.1
EMBL· GenBank· DDBJ
mRNA
AL844566
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AL844573
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC024124
EMBL· GenBank· DDBJ
AAH24124.1
EMBL· GenBank· DDBJ
mRNA
BC030875
EMBL· GenBank· DDBJ
AAH30875.1
EMBL· GenBank· DDBJ
mRNA
BC092291
EMBL· GenBank· DDBJ
AAH92291.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp