Q64408 · C11B1_CAVPO

  • Protein
    Cytochrome P450 11B1, mitochondrial
  • Gene
    CYP11B1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    5/5

Function

function

A cytochrome P450 monooxygenase involved in the biosynthesis of adrenal corticoids (By similarity).
Catalyzes a variety of reactions that are essential for many species, including detoxification, defense, and the formation of endogenous chemicals like steroid hormones (By similarity).
Steroid 11beta, 18- and 19-hydroxylase with preferred regioselectivity at 11beta, then 18, and lastly 19. Catalyzes the hydroxylation of 11-deoxycortisol and 11-deoxycorticosterone (21-hydroxyprogesterone) at 11beta position, yielding cortisol or corticosterone, respectively, but cannot produce aldosterone (By similarity).
Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate for hydroxylation and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Due to its lack of 18-oxidation activity, it is incapable of generating aldosterone. Could also be involved in the androgen metabolic pathway (By similarity).

Catalytic activity

  • a steroid + 2 H+ + O2 + 2 reduced [adrenodoxin] = an 11beta-hydroxysteroid + H2O + 2 oxidized [adrenodoxin]
    This reaction proceeds in the forward direction.
    EC:1.14.15.4 (UniProtKB | ENZYME | Rhea)
  • 11-deoxycortisol + 2 H+ + O2 + 2 reduced [adrenodoxin] = cortisol + H2O + 2 oxidized [adrenodoxin]
    This reaction proceeds in the forward direction.
  • 21-hydroxyprogesterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = corticosterone + H2O + 2 oxidized [adrenodoxin]
    This reaction proceeds in the forward direction.
  • 21-hydroxyprogesterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = 18-hydroxy-11-deoxycorticosterone + H2O + 2 oxidized [adrenodoxin]
    This reaction proceeds in the forward direction.
  • 21-hydroxyprogesterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = 19-hydroxy-11-deoxycorticosterone + H2O + 2 oxidized [adrenodoxin]
    This reaction proceeds in the forward direction.
  • cortisol + 2 H+ + O2 + 2 reduced [adrenodoxin] = 18-hydroxycortisol + H2O + 2 oxidized [adrenodoxin]
    This reaction proceeds in the forward direction.
  • 11-deoxycortisol + 2 H+ + O2 + 2 reduced [adrenodoxin] = 18-hydroxy-11-deoxycortisol + H2O + 2 oxidized [adrenodoxin]
    This reaction proceeds in the forward direction.

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Pathway

Steroid biosynthesis; glucocorticoid biosynthesis.
Steroid hormone biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site447Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentmitochondrial inner membrane
Molecular Functioncorticosterone 18-monooxygenase activity
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionsteroid 11-beta-monooxygenase activity
Biological Processaldosterone biosynthetic process
Biological Processcellular response to peptide hormone stimulus
Biological Processcholesterol metabolic process
Biological Processcortisol metabolic process
Biological Processglucocorticoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytochrome P450 11B1, mitochondrial
  • Alternative names
    • Aldosterone synthase
    • CYPXIB1
    • Cytochrome P450C11
    • Steroid 11-beta-hydroxylase, CYP11B1
      (EC:1.14.15.4
      ) . EC:1.14.15.4 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      CYP11B1

Organism names

  • Taxonomic identifier
  • Strain
    • Dunkin-Hartley
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Hystricomorpha > Caviidae > Cavia

Accessions

  • Primary accession
    Q64408

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-24Mitochondrion
ChainPRO_000000359525-500Cytochrome P450 11B1, mitochondrial

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    500
  • Mass (Da)
    57,820
  • Last updated
    1996-11-01 v1
  • Checksum
    C4B5AB6041CD4AF2
MAFRLKSDVRLAGSWLCLRGARALGTRAVTASKASVLPFEVIPQHQGNKRQRVLQFWKEQNHDDLHLEMHQTFQELGPIFRCDVGSTRIVAVMLPEDCARLHQAESPYPHRMHLEPWMAYREHRRQNLGVFLLNGPEWLSNRRWLNPNVLSPKAVQNLLPMVDTVARDFSEALKQKVLQSAQGSLTMDMQPDIHKYTVEVSNFALFGERLGLFGCNPSSQSLKFIHALEAVFKTTTQLMFLPRSLSRWMRSQAWKEHFEAWDYISEYAENRIQKKYEELARGCSQYNSIVANLMLQGNLPLRAMKANIMDLVAGSVDTTALPLMMTLFELARNPTVQQALRQESMATEPNIYENPQRLRMELPLLWAAIKETLRMYPVGLFLERFLTSPLVLQNYHIPAGTLVHLNLYSMGRNPEVFLSPEHYNPQRWLENKETYKHLAFGFGVRQCIGRRLAEVEMLLFLHHVLKSFCVETAFQEDVKFAYRFVMMPTSAPLLTFRPVS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z69785
EMBL· GenBank· DDBJ
CAA93633.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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