Q643R3 · LPCT4_HUMAN
- ProteinLysophospholipid acyltransferase LPCAT4
- GeneLPCAT4
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids524 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Displays acyl-CoA-dependent lysophospholipid acyltransferase activity with a subset of lysophospholipids as substrates; converts lysophosphatidylethanolamine to phosphatidylethanolamine, lysophosphatidylcholine to phosphatidycholine, 1-alkenyl-lysophatidylethanolamine to 1-alkenyl-phosphatidylethanolamine, lysophosphatidylglycerol and alkyl-lysophosphatidylcholine to phosphatidylglycerol and alkyl-phosphatidylcholine, respectively. In contrast, has no lysophosphatidylinositol, glycerol-3-phosphate, diacylglycerol or lysophosphatidic acid acyltransferase activity. Prefers long chain acyl-CoAs (C16, C18) as acyl donors.
Catalytic activity
- a 1-acyl-sn-glycero-3-phosphoethanolamine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CoA
- a 1-acyl-sn-glycero-3-phosphoethanolamine + octanoyl-CoA = 1-acyl-2-octanoyl-sn-glycero-3-phosphoethanolamine + CoAThis reaction proceeds in the forward direction.
- a 1-acyl-sn-glycero-3-phosphoethanolamine + hexadecanoyl-CoA = 1-acyl-2-hexadecanoyl-sn-glycero-3-phosphoethanolamine + CoAThis reaction proceeds in the forward direction.
- a 1-acyl-sn-glycero-3-phosphoethanolamine + octadecanoyl-CoA = 1-acyl-2-octadecanoyl-sn-glycero-3-phosphoethanolamine + CoAThis reaction proceeds in the forward direction.
- (9Z)-octadecenoyl-CoA + a 1-acyl-sn-glycero-3-phosphoethanolamine = 1-acyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphoethanolamine + CoAThis reaction proceeds in the forward direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + a 1-acyl-sn-glycero-3-phosphoethanolamine = 1-acyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphoethanolamine + CoAThis reaction proceeds in the forward direction.
- 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine + octanoyl-CoA = 1-O-(1Z)-alkenyl-2-octanoyl-sn-glycero-3-phosphoethanolamine + CoAThis reaction proceeds in the forward direction.
- 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine + hexadecanoyl-CoA = 1-O-(1Z)-alkenyl-2-hexadecanoyl-sn-glycero-3-phosphoethanolamine + CoAThis reaction proceeds in the forward direction.
- 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine + octadecanoyl-CoA = 1-O-(1Z)-alkenyl-2-octadecanoyl-sn-glycero-3-phosphoethanolamine + CoAThis reaction proceeds in the forward direction.
- (9Z)-octadecenoyl-CoA + 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine = 1-O-(1Z)-alkenyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphoethanolamine + CoAThis reaction proceeds in the forward direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine = 1-O-(1Z)-alkenyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphoethanolamine + CoAThis reaction proceeds in the forward direction.
- a 1-acyl-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1-acyl-2-hexadecanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- (9Z)-octadecenoyl-CoA + a 1-acyl-sn-glycero-3-phosphocholine = 1-acyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- (9Z)-octadecenoyl-CoA + 1-O-hexadecyl-sn-glycero-3-phosphocholine = 1-O-hexadecyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 1-O-hexadecyl-sn-glycero-3-phosphocholine = 1-O-hexadecyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- (9Z)-octadecenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phospho-L-serine = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + CoAThis reaction proceeds in the forward direction.
- (9Z)-octadecenoyl-CoA + 1-octadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) = 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + CoAThis reaction proceeds in the forward direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 1-octadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + CoAThis reaction proceeds in the forward direction.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
80 μM | lysophosphatidylethanolamine | |||||
20 μM | oleoyl-CoA |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
270 pmol/min/mg | with lysophosphatidylethanolamine and oleoyl-CoA as substrates |
Pathway
Lipid metabolism; phospholipid metabolism.
GO annotations
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameLysophospholipid acyltransferase LPCAT4
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ643R3
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 40-62 | Helical | ||||
Sequence: CLLGALLAPIRVLLAFIVLFLLW | ||||||
Transmembrane | 87-107 | Helical | ||||
Sequence: TVCHNGVLGLSRLLFFLLGFL |
Keywords
- Cellular component
Disease & Variants
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 522 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data), glycosylation.
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000247054 | 1-524 | UniProt | Lysophospholipid acyltransferase LPCAT4 | |||
Sequence: MSQGSPGDWAPLDPTPGPPASPNPFVHELHLSRLQRVKFCLLGALLAPIRVLLAFIVLFLLWPFAWLQVAGLSEEQLQEPITGWRKTVCHNGVLGLSRLLFFLLGFLRIRVRGQRASRLQAPVLVAAPHSTFFDPIVLLPCDLPKVVSRAENLSVPVIGALLRFNQAILVSRHDPASRRRVVEEVRRRATSGGKWPQVLFFPEGTCSNKKALLKFKPGAFIAGVPVQPVLIRYPNSLDTTSWAWRGPGVLKVLWLTASQPCSIVDVEFLPVYHPSPEESRDPTLYANNVQRVMAQALGIPATECEFVGSLPVIVVGRLKVALEPQLWELGKVLRKAGLSAGYVDAGAEPGRSRMISQEEFARQLQLSDPQTVAGAFGYFQQDTKGLVDFRDVALALAALDGGRSLEELTRLAFELFAEEQAEGPNRLLYKDGFSTILHLLLGSPHPAATALHAELCQAGSSQGLSLCQFQNFSLHDPLYGKLFSTYLRPPHTSRGTSQTPNASSPGNPTALANGTVQAPKQKGD | |||||||
Modified residue (large scale data) | 2 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 5 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Glycosylation | 152 | UniProt | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | |||||||
Modified residue (large scale data) | 503 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 504 | PRIDE | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Widely expressed with predominant level in brain.
Gene expression databases
Organism-specific databases
Structure
Family & Domains
Features
Showing features for motif, compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 129-134 | HXXXXD motif | ||||
Sequence: HSTFFD | ||||||
Compositional bias | 489-516 | Polar residues | ||||
Sequence: PPHTSRGTSQTPNASSPGNPTALANGTV | ||||||
Region | 489-524 | Disordered | ||||
Sequence: PPHTSRGTSQTPNASSPGNPTALANGTVQAPKQKGD |
Sequence similarities
Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length524
- Mass (Da)57,219
- Last updated2004-10-25 v1
- Checksum3A2A12656E1241BC
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
H3BMK4 | H3BMK4_HUMAN | LPCAT4 | 84 |
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 489-516 | Polar residues | ||||
Sequence: PPHTSRGTSQTPNASSPGNPTALANGTV |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY734233 EMBL· GenBank· DDBJ | AAU34184.1 EMBL· GenBank· DDBJ | mRNA | ||
AF529233 EMBL· GenBank· DDBJ | AAP97722.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AF542964 EMBL· GenBank· DDBJ | AAN33178.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AK290273 EMBL· GenBank· DDBJ | BAF82962.1 EMBL· GenBank· DDBJ | mRNA | ||
CH471125 EMBL· GenBank· DDBJ | EAW92306.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC024892 EMBL· GenBank· DDBJ | AAH24892.2 EMBL· GenBank· DDBJ | mRNA | ||
BC092463 EMBL· GenBank· DDBJ | AAH92463.1 EMBL· GenBank· DDBJ | mRNA | ||
AF007155 EMBL· GenBank· DDBJ | AAC19156.1 EMBL· GenBank· DDBJ | mRNA | Sequence problems. |