Q64343 · ABCG1_MOUSE
- ProteinATP-binding cassette sub-family G member 1
- GeneAbcg1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids666 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrolysis of ATP (PubMed:14668945).
The lipid efflux is ALB-dependent. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol (By similarity).
The lipid efflux is ALB-dependent. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol (By similarity).
Catalytic activity
- 7beta-hydroxycholesterol(in) + ATP + H2O = 7beta-hydroxycholesterol(out) + ADP + H+ + phosphate
- ATP + cholesterol(in) + H2O = ADP + cholesterol(out) + H+ + phosphate
- an N-(acyl)-sphingosylphosphocholine(in) + ATP + H2O = ADP + an N-(acyl)-sphingosylphosphocholine(out) + H+ + phosphate
- a sphingomyelin(in) + ATP + H2O = a sphingomyelin(out) + ADP + H+ + phosphate
Activity regulation
The cholesterol efflux is enhanced by APOA1.
Features
Showing features for binding site.
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-binding cassette sub-family G member 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ64343
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Golgi apparatus membrane ; Multi-pass membrane protein
Note: Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-414 | Cytoplasmic | ||||
Sequence: MACLMAAFSVGTAMNASSYSAAMTEPKSVCVSVDEVVSSNVDEVETDLLNGHLKKVDNNFTEAQRFSSLPRRAAVNIEFKDLSYSVPEGPWWKKKGYKTLLKGISGKFNSGELVAIMGPSGAGKSTLMNILAGYRETGMKGAVLINGMPRDLRCFRKVSCYIMQDDMLLPHLTVQEAMMVSAHLKLQEKDEGRREMVKEILTALGLLPCANTRTGSLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKGLAQGGRSIVCTIHQPSAKLFELFDQLYVLSQGQCVYRGKVSNLVPYLRDLGLNCPTYHNPADFVMEVASGEYGDQNSRLVRAVREGMCDADYKRDLGGDTDVNPFLWHRPAEEDSASMEGCHSFSASCLTQFCILFKRTFLSIMRDSVLTHLR | ||||||
Transmembrane | 415-433 | Helical | ||||
Sequence: ITSHIGIGLLIGLLYLGIG | ||||||
Topological domain | 434-444 | Extracellular | ||||
Sequence: NEAKKVLSNSG | ||||||
Transmembrane | 445-465 | Helical | ||||
Sequence: FLFFSMLFLMFAALMPTVLTF | ||||||
Topological domain | 466-494 | Cytoplasmic | ||||
Sequence: PLEMSVFLREHLNYWYSLKAYYLAKTMAD | ||||||
Transmembrane | 495-513 | Helical | ||||
Sequence: VPFQIMFPVAYCSIVYWMT | ||||||
Topological domain | 514-521 | Extracellular | ||||
Sequence: SQPSDAVR | ||||||
Transmembrane | 522-543 | Helical | ||||
Sequence: FVLFAALGTMTSLVAQSLGLLI | ||||||
Topological domain | 544-555 | Cytoplasmic | ||||
Sequence: GAASTSLQVATF | ||||||
Transmembrane | 556-574 | Helical | ||||
Sequence: VGPVTAIPVLLFSGFFVSF | ||||||
Topological domain | 575-637 | Extracellular | ||||
Sequence: DTIPAYLQWMSYISYVRYGFEGVILSIYGLDREDLHCDIAETCHFQKSEAILRELDVENAKLY | ||||||
Transmembrane | 638-657 | Helical | ||||
Sequence: LDFIVLGIFFISLRLIAYFV | ||||||
Topological domain | 658-666 | Cytoplasmic | ||||
Sequence: LRYKIRAER |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, lipidation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000093385 | 1-666 | ATP-binding cassette sub-family G member 1 | |||
Sequence: MACLMAAFSVGTAMNASSYSAAMTEPKSVCVSVDEVVSSNVDEVETDLLNGHLKKVDNNFTEAQRFSSLPRRAAVNIEFKDLSYSVPEGPWWKKKGYKTLLKGISGKFNSGELVAIMGPSGAGKSTLMNILAGYRETGMKGAVLINGMPRDLRCFRKVSCYIMQDDMLLPHLTVQEAMMVSAHLKLQEKDEGRREMVKEILTALGLLPCANTRTGSLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKGLAQGGRSIVCTIHQPSAKLFELFDQLYVLSQGQCVYRGKVSNLVPYLRDLGLNCPTYHNPADFVMEVASGEYGDQNSRLVRAVREGMCDADYKRDLGGDTDVNPFLWHRPAEEDSASMEGCHSFSASCLTQFCILFKRTFLSIMRDSVLTHLRITSHIGIGLLIGLLYLGIGNEAKKVLSNSGFLFFSMLFLMFAALMPTVLTFPLEMSVFLREHLNYWYSLKAYYLAKTMADVPFQIMFPVAYCSIVYWMTSQPSDAVRFVLFAALGTMTSLVAQSLGLLIGAASTSLQVATFVGPVTAIPVLLFSGFFVSFDTIPAYLQWMSYISYVRYGFEGVILSIYGLDREDLHCDIAETCHFQKSEAILRELDVENAKLYLDFIVLGIFFISLRLIAYFVLRYKIRAER | ||||||
Lipidation | 30 | S-palmitoyl cysteine | ||||
Sequence: C | ||||||
Lipidation | 154 | S-palmitoyl cysteine | ||||
Sequence: C | ||||||
Lipidation | 315 | S-palmitoyl cysteine | ||||
Sequence: C | ||||||
Lipidation | 394 | S-palmitoyl cysteine | ||||
Sequence: C |
Post-translational modification
Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed mainly in brain, thymus, lung, adrenals, spleen and placenta. Little or no expression in liver, kidney, heart, muscle or testes.
Induction
Strongly induced in macrophage cell line RAW 264.7 during cholesterol influx. Induction is mediated by the liver X receptor/retinoid X receptor (LXR/RXR) pathway. Down-regulated by endotoxins or cytokines (TNF and IL1) in J-774 macrophages.
Gene expression databases
Interaction
Subunit
Homodimer; disulfide-linked. Homooligomer. May form heterodimers with several heterologous partners of the ABCG subfamily. Forms heterodimers with ABCG4. Interacts with CAV1; this interaction regulates ABCG1-mediated cholesterol efflux.
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 77-317 | ABC transporter | ||||
Sequence: IEFKDLSYSVPEGPWWKKKGYKTLLKGISGKFNSGELVAIMGPSGAGKSTLMNILAGYRETGMKGAVLINGMPRDLRCFRKVSCYIMQDDMLLPHLTVQEAMMVSAHLKLQEKDEGRREMVKEILTALGLLPCANTRTGSLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKGLAQGGRSIVCTIHQPSAKLFELFDQLYVLSQGQCVYRGKVSNLVPYLRDLGLNCPT | ||||||
Domain | 403-661 | ABC transmembrane type-2 | ||||
Sequence: SIMRDSVLTHLRITSHIGIGLLIGLLYLGIGNEAKKVLSNSGFLFFSMLFLMFAALMPTVLTFPLEMSVFLREHLNYWYSLKAYYLAKTMADVPFQIMFPVAYCSIVYWMTSQPSDAVRFVLFAALGTMTSLVAQSLGLLIGAASTSLQVATFVGPVTAIPVLLFSGFFVSFDTIPAYLQWMSYISYVRYGFEGVILSIYGLDREDLHCDIAETCHFQKSEAILRELDVENAKLYLDFIVLGIFFISLRLIAYFVLRYK |
Sequence similarities
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length666
- Mass (Da)74,033
- Last updated1997-11-01 v1
- ChecksumEDDC6AFBD43950B6
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A494B9N8 | A0A494B9N8_MOUSE | Abcg1 | 257 | ||
A0A494B9K1 | A0A494B9K1_MOUSE | Abcg1 | 104 | ||
A0A494BA50 | A0A494BA50_MOUSE | Abcg1 | 146 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U34920 EMBL· GenBank· DDBJ | AAB47738.1 EMBL· GenBank· DDBJ | mRNA | ||
Z48745 EMBL· GenBank· DDBJ | CAA88636.1 EMBL· GenBank· DDBJ | mRNA | ||
AF323659 EMBL· GenBank· DDBJ | AAK27442.1 EMBL· GenBank· DDBJ | mRNA |