Q64028 · PHC1_MOUSE
- ProteinPolyhomeotic-like protein 1
- GenePhc1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1010 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Required for proper control of cellular levels of GMNN expression (By similarity).
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nuclear body | |
Cellular Component | nucleus | |
Cellular Component | PRC1 complex | |
Cellular Component | sex chromatin | |
Molecular Function | chromatin binding | |
Molecular Function | DNA binding | |
Molecular Function | histone binding | |
Molecular Function | zinc ion binding | |
Biological Process | cellular response to leukemia inhibitory factor | |
Biological Process | cellular response to retinoic acid | |
Biological Process | chromatin remodeling | |
Biological Process | negative regulation of DNA-templated transcription |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePolyhomeotic-like protein 1
- Short namesmPH1
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ64028
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000058376 | 1-1010 | Polyhomeotic-like protein 1 | |||
Sequence: METESEQNSSSTNGSSSSGASSRPQIAQMSLYERQAVQALQALQRQPNAAQYFHQFMLQQQLSNAQLHSLAAVQQATIAASRQASSPNSSTAQQQTATTQASMNLATTSAAQLISRSQSVSSPSATTLTQSVLLGNTTSPPLNQSQAQMYLRPQLGNLLQVNRTLGRNVPLASQLILMPNGAVAAVQQEVPPAQSPGVHADADQVQNLAVRNQQASAQGPQMPGSTQKAIPPGASPVSGLSQTSSQALAVAQASSGASGQSLNLSQAGGGSGNSLPGSMGPGGGGQAPGGLGQLPSSGLTGGSCPRKGTGVVQPLPAAQTVTVSQGSQTEAESAAAKKAEADGSGQQSVGMNLTRTATPAPSQTLISSATYTQIQPHSLIQQQQQIHLQQKQVVIQQQIAIHHQQQFQHRQSQLLHTATHLQLAQQQQQQQQQQQQQQQQQQQQQQGTTLTAPQPPQVPPTQQVPPSQSQQQAQTLVVQPMLQSSPLTLPPEPTSKPPIPIQSKPPVAPIKPPQLGAAKMSATQQPPPHIPVQVVGTRQPGSAQAQALGLAQLAAAVPTPRGITGAVQPGQAHLASSPPSSQAAPGALQECPPALAAGMTLAPVQGTAHVVKGGPTASSPVVAQVPAAFYMQSVHLPGKAQTLAVKRKAESEEERDDLSALASVLPTKASPAAESPKVIEEKNSLGEKAEPVASLNANPPNSDLVALAPTPSAPPPTLALVSRQMGDSKPPQAIVKPQILTHIIEGFVIQEGAEPFPVGCSQFLKETKKPLQAGLPTGLNESQPSGPLGGDSPSVELEKKANLLKCEYCGKYAPAEQFRGSKRFCSMTCAKRYNVSCSHQFRLKRKKMKEFQEASYARVRRRGPRRSSSDIARAKIQGKRHRGQEDSSRGSDNSSYDEALSPTSPGPLSVRAGHGERDLGNTITTPSTPELQGINPVFLSSNPSQWSVEEVYEFIASLQGCQEIAEEFRSQEIDGQALLLLKEEHLMSAMNIKLGPALKICAKINVLKET | ||||||
Modified residue | 651 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 769 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Modified residue | 904 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 928 | Phosphothreonine | ||||
Sequence: T |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Highly expressed in testis with lower levels in most other tissues. Expressed in embryonic stem cells (PubMed:22226355).
Induction
By retinoic acid.
Developmental stage
Expressed ubiquitously in 8.5 dpc embryos. At 10.5 dpc, strongly expressed in pharyngeal arches and weakly expressed in heart. By 14.5 dpc, expression is detected throughout the central nervous system.
Gene expression databases
Interaction
Subunit
Homodimer. Component of a PRC1-like complex (By similarity).
Interacts with the SAM domain of SCMH1 via its SAM domain in vitro (PubMed:10653359).
Interacts with RNF2 and CBX7 (PubMed:22226355).
Interacts with PHC2 (PubMed:16024804).
Interacts with BMI1 (PubMed:9009205).
Interacts with the SAM domain of SCMH1 via its SAM domain in vitro (PubMed:10653359).
Interacts with RNF2 and CBX7 (PubMed:22226355).
Interacts with PHC2 (PubMed:16024804).
Interacts with BMI1 (PubMed:9009205).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q64028 | Mapkapk2 P49138 | 2 | EBI-927346, EBI-298776 | |
BINARY | Q64028 | Pcgf2 P23798 | 4 | EBI-927346, EBI-926857 | |
BINARY | Q64028 | Phc2 Q9QWH1 | 2 | EBI-927346, EBI-642357 | |
BINARY | Q64028 | Rnf2 Q9CQJ4 | 7 | EBI-927346, EBI-927321 | |
BINARY | Q64028 | Scmh1 Q8K214 | 2 | EBI-927346, EBI-445955 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias, zinc finger, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-25 | Disordered | ||||
Sequence: METESEQNSSSTNGSSSSGASSRPQ | ||||||
Region | 212-243 | Disordered | ||||
Sequence: NQQASAQGPQMPGSTQKAIPPGASPVSGLSQT | ||||||
Compositional bias | 259-273 | Polar residues | ||||
Sequence: GQSLNLSQAGGGSGN | ||||||
Region | 259-312 | Disordered | ||||
Sequence: GQSLNLSQAGGGSGNSLPGSMGPGGGGQAPGGLGQLPSSGLTGGSCPRKGTGVV | ||||||
Region | 444-506 | Disordered | ||||
Sequence: QQQGTTLTAPQPPQVPPTQQVPPSQSQQQAQTLVVQPMLQSSPLTLPPEPTSKPPIPIQSKPP | ||||||
Compositional bias | 464-485 | Polar residues | ||||
Sequence: VPPSQSQQQAQTLVVQPMLQSS | ||||||
Compositional bias | 486-506 | Pro residues | ||||
Sequence: PLTLPPEPTSKPPIPIQSKPP | ||||||
Region | 565-588 | Disordered | ||||
Sequence: GAVQPGQAHLASSPPSSQAAPGAL | ||||||
Compositional bias | 570-584 | Polar residues | ||||
Sequence: GQAHLASSPPSSQAA | ||||||
Region | 646-678 | Disordered | ||||
Sequence: KRKAESEEERDDLSALASVLPTKASPAAESPKV | ||||||
Region | 772-794 | Disordered | ||||
Sequence: QAGLPTGLNESQPSGPLGGDSPS | ||||||
Zinc finger | 797-831 | FCS-type | ||||
Sequence: LEKKANLLKCEYCGKYAPAEQFRGSKRFCSMTCAK | ||||||
Compositional bias | 854-892 | Basic and acidic residues | ||||
Sequence: ASYARVRRRGPRRSSSDIARAKIQGKRHRGQEDSSRGSD | ||||||
Region | 854-928 | Disordered | ||||
Sequence: ASYARVRRRGPRRSSSDIARAKIQGKRHRGQEDSSRGSDNSSYDEALSPTSPGPLSVRAGHGERDLGNTITTPST | ||||||
Domain | 946-1010 | SAM | ||||
Sequence: WSVEEVYEFIASLQGCQEIAEEFRSQEIDGQALLLLKEEHLMSAMNIKLGPALKICAKINVLKET |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing.
Q64028-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length1,010
- Mass (Da)106,060
- Last updated2022-12-14 v3
- Checksum086BB97C290243C8
Q64028-2
- Name2
- Differences from canonical
- 153-159: Missing
Q64028-3
- Name3
- Differences from canonical
- 153-204: Missing
Computationally mapped potential isoform sequences
There are 8 potential isoforms mapped to this entry
Features
Showing features for sequence conflict, alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 127 | in Ref. 2; AAC28974 | ||||
Sequence: T → A | ||||||
Sequence conflict | 131 | in Ref. 2; AAC28974 | ||||
Sequence: S → W | ||||||
Alternative sequence | VSP_004040 | 153-159 | in isoform 2 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_004041 | 153-204 | in isoform 3 | |||
Sequence: Missing | ||||||
Compositional bias | 259-273 | Polar residues | ||||
Sequence: GQSLNLSQAGGGSGN | ||||||
Sequence conflict | 401 | in Ref. 1; AAB31766 | ||||
Sequence: I → Y | ||||||
Sequence conflict | 446 | in Ref. 1; AAB31766, 4; AAH46535 and 2; AAC28974 | ||||
Sequence: Q → QQQ | ||||||
Compositional bias | 464-485 | Polar residues | ||||
Sequence: VPPSQSQQQAQTLVVQPMLQSS | ||||||
Compositional bias | 486-506 | Pro residues | ||||
Sequence: PLTLPPEPTSKPPIPIQSKPP | ||||||
Compositional bias | 570-584 | Polar residues | ||||
Sequence: GQAHLASSPPSSQAA | ||||||
Sequence conflict | 768 | in Ref. 1; AAB31766, 4; AAH46535 and 2; AAC28974 | ||||
Sequence: K → E | ||||||
Compositional bias | 854-892 | Basic and acidic residues | ||||
Sequence: ASYARVRRRGPRRSSSDIARAKIQGKRHRGQEDSSRGSD |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
S73882 EMBL· GenBank· DDBJ | AAB31766.1 EMBL· GenBank· DDBJ | mRNA | ||
U63386 EMBL· GenBank· DDBJ | AAC28974.1 EMBL· GenBank· DDBJ | mRNA | ||
AC153579 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC046535 EMBL· GenBank· DDBJ | AAH46535.1 EMBL· GenBank· DDBJ | mRNA | ||
BC052394 EMBL· GenBank· DDBJ | AAH52394.1 EMBL· GenBank· DDBJ | mRNA | ||
AK036370 EMBL· GenBank· DDBJ | BAC29400.1 EMBL· GenBank· DDBJ | mRNA |