Q63042 · ALR_RAT

  • Protein
    FAD-linked sulfhydryl oxidase ALR
  • Gene
    Gfer
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. May have a function in liver regeneration and spermatogenesis.

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Features

Showing features for binding site.

119820406080100120140160180
TypeIDPosition(s)Description
Binding site92-100FAD (UniProtKB | ChEBI)
Binding site104FAD (UniProtKB | ChEBI)
Binding site133FAD (UniProtKB | ChEBI)
Binding site164-176FAD (UniProtKB | ChEBI)
Binding site187-188FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentextracellular space
Cellular Componentmitochondrial intermembrane space
Cellular Componentmitochondrion
Molecular Functionflavin adenine dinucleotide binding
Molecular Functionflavin-dependent sulfhydryl oxidase activity
Molecular Functiongrowth factor activity
Molecular Functionprotein-disulfide reductase activity
Biological Processcellular response to actinomycin D
Biological Processcellular response to lipopolysaccharide
Biological Processcellular response to toxic substance
Biological Processcellular response to tumor necrosis factor
Biological Processliver development
Biological Processliver regeneration
Biological Processnegative regulation of apoptotic process
Biological Processnegative regulation of natural killer cell mediated cytotoxicity
Biological Processpositive regulation of DNA biosynthetic process
Biological Processresponse to lipopolysaccharide

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    FAD-linked sulfhydryl oxidase ALR
  • EC number
  • Alternative names
    • Augmenter of liver regeneration

Gene names

    • Name
      Gfer
    • Synonyms
      Alr

Organism names

  • Taxonomic identifier
  • Strains
    • Fischer 344
    • Wistar
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    Q63042

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00002085501-198FAD-linked sulfhydryl oxidase ALR
Disulfide bond88Interchain (with C-197)
Disulfide bond135↔138Redox-active
Disulfide bond164↔181
Disulfide bond197Interchain (with C-88)

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Homodimer; disulfide-linked (PubMed:12717032, PubMed:22948913).
Interacts with CHCHD4/MIA40 (By similarity).

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region1-59Disordered
Compositional bias29-45Basic and acidic residues
Domain88-188ERV/ALR sulfhydryl oxidase

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    198
  • Mass (Da)
    22,837
  • Last updated
    2008-12-16 v2
  • Checksum
    46AD9BEC2EF0C393
MAAPSEPAGFPRGSRFSFLPGGAHSEMTDDLVTDARGRGARHRKDNAPAAAPAPKGLEHGKRPCRACVDFKSWMRTQQKRDIKFREDCPQDREELGRNTWAFLHTLAAYYPDMPTPEQQQDMAQFIHIFSKFYPCEECAEDIRKRIDRSQPDTSTRVSFSQWLCRLHNEVNRKLGKPDFDCSRVDERWRDGWKDGSCD

Sequence caution

The sequence AAF12808.1 differs from that shown. Reason: Erroneous initiation
The sequence BAA06399.1 differs from that shown. Reason: Erroneous initiation

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias29-45Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D30735
EMBL· GenBank· DDBJ
BAA06399.1
EMBL· GenBank· DDBJ
mRNA Different initiation
AF197192
EMBL· GenBank· DDBJ
AAF12808.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

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