Q62377 · U2AFM_MOUSE
- ProteinU2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2
- GeneZrsr2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids462 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Pre-mRNA-binding protein required for splicing of both U2- and U12-type introns. Selectively interacts with the 3'-splice site of U2- and U12-type pre-mRNAs and promotes different steps in U2 and U12 intron splicing. Recruited to U12 pre-mRNAs in an ATP-dependent manner and is required for assembly of the prespliceosome, a precursor to other spliceosomal complexes. For U2-type introns, it is selectively and specifically required for the second step of splicing (By similarity).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleolus | |
Cellular Component | spliceosomal complex | |
Cellular Component | U12-type spliceosomal complex | |
Cellular Component | U2AF complex | |
Molecular Function | metal ion binding | |
Molecular Function | pre-mRNA 3'-splice site binding | |
Biological Process | mRNA splicing, via spliceosome | |
Biological Process | spliceosomal complex assembly |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameU2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ62377
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain, cross-link, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000082002 | 1-462 | U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2 | |||
Sequence: METAGATADATAGPQKLSRKKYLALRKKERRKRRRQALARLREAELAQKEEEEDPLAEEKRLEEERLLEEERQRLHEEWLLREEKAQEEFRAKKKKEEEARKRKEELERKLKAEWEEQQRKEREEEEQKRQEKREREEAVQKMLDQAENELENGGTWQNPEPPMDIRVLEKDRANCPFYSKTGACRFGDRCSRKHNFPTSSPTLLIKGMFTTFGMEQCRRDDYDPDSSLEFSEEEIYQQFLDFYYDVLPEFKSVGKVIQFKVSCNLEPHLRGNVYVQYQSEEDCQAAFSVFNGRWYAGRQLQCEFCPVTRWKMAICGLFEVQQCPRGKHCNFLHVFRNPNNEYRDANRDLYPSPDWTSSSFGKNSERRERASHYDEYYGRSRRRRRSPSPDFYKRNGESDRKSSSRHRVKKSHRYGMKSRERRSSPSRRRKDHSPGPWEPEQEEPPQQESQSQPQPQPQSDP | ||||||
Cross-link | 49 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Modified residue | 353 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 389 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Phosphorylated in the RS domain by SRPK1.
Keywords
- PTM
Proteomic databases
PTM databases
Structure
Family & Domains
Features
Showing features for region, zinc finger, domain, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-22 | Disordered | ||||
Sequence: METAGATADATAGPQKLSRKKY | ||||||
Region | 44-66 | Disordered | ||||
Sequence: AELAQKEEEEDPLAEEKRLEEER | ||||||
Region | 115-138 | Disordered | ||||
Sequence: WEEQQRKEREEEEQKRQEKREREE | ||||||
Zinc finger | 170-198 | C3H1-type 1 | ||||
Sequence: EKDRANCPFYSKTGACRFGDRCSRKHNFP | ||||||
Domain | 202-308 | RRM | ||||
Sequence: PTLLIKGMFTTFGMEQCRRDDYDPDSSLEFSEEEIYQQFLDFYYDVLPEFKSVGKVIQFKVSCNLEPHLRGNVYVQYQSEEDCQAAFSVFNGRWYAGRQLQCEFCPV | ||||||
Zinc finger | 310-337 | C3H1-type 2 | ||||
Sequence: RWKMAICGLFEVQQCPRGKHCNFLHVFR | ||||||
Region | 354-462 | Disordered | ||||
Sequence: PDWTSSSFGKNSERRERASHYDEYYGRSRRRRRSPSPDFYKRNGESDRKSSSRHRVKKSHRYGMKSRERRSSPSRRRKDHSPGPWEPEQEEPPQQESQSQPQPQPQSDP | ||||||
Compositional bias | 362-405 | Basic and acidic residues | ||||
Sequence: GKNSERRERASHYDEYYGRSRRRRRSPSPDFYKRNGESDRKSSS | ||||||
Compositional bias | 406-427 | Basic residues | ||||
Sequence: RHRVKKSHRYGMKSRERRSSPS | ||||||
Compositional bias | 444-462 | Polar residues | ||||
Sequence: EPPQQESQSQPQPQPQSDP |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length462
- Mass (Da)55,358
- Last updated1996-11-01 v1
- Checksum0E980C029ACBA196
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 362-405 | Basic and acidic residues | ||||
Sequence: GKNSERRERASHYDEYYGRSRRRRRSPSPDFYKRNGESDRKSSS | ||||||
Compositional bias | 406-427 | Basic residues | ||||
Sequence: RHRVKKSHRYGMKSRERRSSPS | ||||||
Compositional bias | 444-462 | Polar residues | ||||
Sequence: EPPQQESQSQPQPQPQSDP |
Keywords
- Technical term