Q62293 · TGTP1_MOUSE
- ProteinT-cell-specific guanine nucleotide triphosphate-binding protein 1
- GeneTgtp1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids415 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in innate cell-autonomous resistance to intracellular pathogens, such as Toxoplasma gondii (PubMed:19265156, PubMed:20109161, PubMed:24563254).
During avirulent type II T. gondii infection, recruited to the parasitophorous vacuole (PV) membrane, leading to PV vesiculation and rupture, and subsequent digestion of the parasite within the cytosol (PubMed:19265156, PubMed:24563254).
Not recruited to virulent type I T. gondii PV membrane (PubMed:19265156).
May confer an antiviral state for vesicular stomatitis virus (PubMed:9725230).
During avirulent type II T. gondii infection, recruited to the parasitophorous vacuole (PV) membrane, leading to PV vesiculation and rupture, and subsequent digestion of the parasite within the cytosol (PubMed:19265156, PubMed:24563254).
Not recruited to virulent type I T. gondii PV membrane (PubMed:19265156).
May confer an antiviral state for vesicular stomatitis virus (PubMed:9725230).
Catalytic activity
- GTP + H2O = GDP + H+ + phosphate
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 66 | GDP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 68 | GDP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 69 | GDP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 70 | GDP (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 90 | GDP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 171 | GDP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 173 | GDP (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 219 | GDP (UniProtKB | ChEBI) | ||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | Golgi apparatus | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Biological Process | autophagosome assembly | |
Biological Process | cellular response to interferon-beta | |
Biological Process | defense response to protozoan | |
Biological Process | immune response | |
Biological Process | innate immune response | |
Biological Process | response to bacterium | |
Biological Process | response to interferon-alpha | |
Biological Process | response to type II interferon | |
Biological Process | response to virus |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameT-cell-specific guanine nucleotide triphosphate-binding protein 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ62293
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: In astrocytes stimulated with IFNG or TNF, diffuse cytoplasmic localization decreases and the protein partially relocalizes to the endoplasmic reticulum and Golgi apparatus (PubMed:19285957).
Due to sequence similarity with Tgtp2, it is impossible to assign unambiguously experimental data published in the literature to Tgtp1 or Tgtp2 gene (Probable)
Due to sequence similarity with Tgtp2, it is impossible to assign unambiguously experimental data published in the literature to Tgtp1 or Tgtp2 gene (Probable)
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000437942 | 1-415 | T-cell-specific guanine nucleotide triphosphate-binding protein 1 | |||
Sequence: MAWASSFDAFFKNFKRESKIISEYDITLIMTYIEENKLQKAVSVIEKVLRDIESAPLHIAVTGETGAGKSTFINTLRGVGHEEKGAAPTGAIETTMKRTPYPHPKLPNVTIWDLPGIGTTNFTPQNYLTEMKFGEYDFFIIISATRFKENDAQLAKAIAQMGMNFYFVRTKIDSDLDNEQKFKPKSFNKEEVLKNIKDYCSNHLQESLDSEPPVFLVSNVDISKYDFPKLETKLLQDLPAHKRHVFSLSLQSLTEATINYKRDSLKQKVFLEAMKAGALATIPLGGMISDILENLDETFNLYRSYFGLDDASLENIAQDLNMSVDDFKVHLRFPHLFAEHNDESLEDKLFKYIKHISSVTGGPVAAVTYYRMAYYLQNLFLDTAANDAIALLNSKALFEKKVGPYISEPPEYWEA |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in thymus and lymph nodes, predominantly T-cells. Not expressed by immature CD4+ CD8+ thymocytes (at protein level) (PubMed:7836757).
Expressed in IFNG-stimulated macrophages (PubMed:7884320).
Expressed at low levels in unstimulated astrocytes (PubMed:19285957).
Due to sequence similarity with Tgtp2, it is impossible to assign unambiguously experimental data published in the literature to Tgtp1 or Tgtp2 gene
Expressed in IFNG-stimulated macrophages (PubMed:7884320).
Expressed at low levels in unstimulated astrocytes (PubMed:19285957).
Due to sequence similarity with Tgtp2, it is impossible to assign unambiguously experimental data published in the literature to Tgtp1 or Tgtp2 gene
Induction
In macrophages, up-regulated by IFNG, but not by IL2, IL4, IL10, nor TNF (PubMed:7884320).
Up-regulated by IFNG in lymph node cells and thymocytes and other cell types (PubMed:24563254, PubMed:7836757, PubMed:9725230).
In astrocytes, up-regulated by TNF and IFNG; when both cytokines are combined, the effect is synergistic (PubMed:19285957).
Due to sequence similarity with Tgtp2, it is impossible to assign unambiguously experimental data published in the literature to Tgtp1 or Tgtp2 gene (Probable)
Up-regulated by IFNG in lymph node cells and thymocytes and other cell types (PubMed:24563254, PubMed:7836757, PubMed:9725230).
In astrocytes, up-regulated by TNF and IFNG; when both cytokines are combined, the effect is synergistic (PubMed:19285957).
Due to sequence similarity with Tgtp2, it is impossible to assign unambiguously experimental data published in the literature to Tgtp1 or Tgtp2 gene (Probable)
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 55-237 | IRG-type G | ||||
Sequence: APLHIAVTGETGAGKSTFINTLRGVGHEEKGAAPTGAIETTMKRTPYPHPKLPNVTIWDLPGIGTTNFTPQNYLTEMKFGEYDFFIIISATRFKENDAQLAKAIAQMGMNFYFVRTKIDSDLDNEQKFKPKSFNKEEVLKNIKDYCSNHLQESLDSEPPVFLVSNVDISKYDFPKLETKLLQD |
Sequence similarities
Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length415
- Mass (Da)47,121
- Last updated1996-11-01 v1
- Checksum3AFB5F940242952A
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 91 | in Ref. 1; AAA66220 | ||||
Sequence: A → G | ||||||
Sequence conflict | 272 | in Ref. 2; BAC40974 | ||||
Sequence: E → G |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U15636 EMBL· GenBank· DDBJ | AAA66220.1 EMBL· GenBank· DDBJ | mRNA | ||
AK089836 EMBL· GenBank· DDBJ | BAC40974.1 EMBL· GenBank· DDBJ | mRNA | ||
AK163978 EMBL· GenBank· DDBJ | BAE37565.1 EMBL· GenBank· DDBJ | mRNA | ||
AK172473 EMBL· GenBank· DDBJ | BAE43026.1 EMBL· GenBank· DDBJ | mRNA | ||
AL627237 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH466575 EMBL· GenBank· DDBJ | EDL33781.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC085259 EMBL· GenBank· DDBJ | AAH85259.1 EMBL· GenBank· DDBJ | mRNA | ||
FR734025 EMBL· GenBank· DDBJ | CBY65988.1 EMBL· GenBank· DDBJ | Genomic DNA |