Q62293 · TGTP1_MOUSE

  • Protein
    T-cell-specific guanine nucleotide triphosphate-binding protein 1
  • Gene
    Tgtp1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Involved in innate cell-autonomous resistance to intracellular pathogens, such as Toxoplasma gondii (PubMed:19265156, PubMed:20109161, PubMed:24563254).
During avirulent type II T. gondii infection, recruited to the parasitophorous vacuole (PV) membrane, leading to PV vesiculation and rupture, and subsequent digestion of the parasite within the cytosol (PubMed:19265156, PubMed:24563254).
Not recruited to virulent type I T. gondii PV membrane (PubMed:19265156).
May confer an antiviral state for vesicular stomatitis virus (PubMed:9725230).

Caution

The gene Tgtp1 belongs to a large family of eutherian IFNG-inducible GTPases, called immunity-related p47 GTPases, which comprises a variable amount of paralogs depending upon the species studied. In C57BL/6J mice, there is over 20 genes, whereas humans have only one ortholog. Tgtp1 closest paralog is Tgtp2. Both genes encode identical proteins. At the nucleotide sequence level, their CDSs differ at only 4 positions. Consequently it is almost impossible to assign unambiguously to one gene or the other experimental data published in the literature.

Catalytic activity

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site66GDP (UniProtKB | ChEBI)
Binding site68GDP (UniProtKB | ChEBI)
Binding site69GDP (UniProtKB | ChEBI)
Binding site70GDP (UniProtKB | ChEBI)
Binding site90GDP (UniProtKB | ChEBI)
Binding site171GDP (UniProtKB | ChEBI)
Binding site173GDP (UniProtKB | ChEBI)
Binding site219GDP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Cellular ComponentGolgi apparatus
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Biological Processautophagosome assembly
Biological Processcellular response to interferon-beta
Biological Processdefense response to protozoan
Biological Processimmune response
Biological Processinnate immune response
Biological Processresponse to bacterium
Biological Processresponse to interferon-alpha
Biological Processresponse to type II interferon
Biological Processresponse to virus

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    T-cell-specific guanine nucleotide triphosphate-binding protein 1
  • EC number
  • Alternative names
    • Interferon-gamma-inducible GTPase Ifggb5

Gene names

    • Name
      Tgtp1
    • Synonyms
      Ifggb5
      , Irgb6
      , Mg21

Organism names

  • Taxonomic identifier
  • Strains
    • BALB/cJ
    • C57BL/6J
    • NOD
    • NMRI
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q62293
  • Secondary accessions
    • Q60711
    • Q8BN19

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Endoplasmic reticulum
Golgi apparatus
Note: In astrocytes stimulated with IFNG or TNF, diffuse cytoplasmic localization decreases and the protein partially relocalizes to the endoplasmic reticulum and Golgi apparatus (PubMed:19285957).
Due to sequence similarity with Tgtp2, it is impossible to assign unambiguously experimental data published in the literature to Tgtp1 or Tgtp2 gene (Probable)

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004379421-415T-cell-specific guanine nucleotide triphosphate-binding protein 1

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in thymus and lymph nodes, predominantly T-cells. Not expressed by immature CD4+ CD8+ thymocytes (at protein level) (PubMed:7836757).
Expressed in IFNG-stimulated macrophages (PubMed:7884320).
Expressed at low levels in unstimulated astrocytes (PubMed:19285957).
Due to sequence similarity with Tgtp2, it is impossible to assign unambiguously experimental data published in the literature to Tgtp1 or Tgtp2 gene

Induction

In macrophages, up-regulated by IFNG, but not by IL2, IL4, IL10, nor TNF (PubMed:7884320).
Up-regulated by IFNG in lymph node cells and thymocytes and other cell types (PubMed:24563254, PubMed:7836757, PubMed:9725230).
In astrocytes, up-regulated by TNF and IFNG; when both cytokines are combined, the effect is synergistic (PubMed:19285957).
Due to sequence similarity with Tgtp2, it is impossible to assign unambiguously experimental data published in the literature to Tgtp1 or Tgtp2 gene (Probable)

Gene expression databases

Interaction

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain55-237IRG-type G

Sequence similarities

Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    415
  • Mass (Da)
    47,121
  • Last updated
    1996-11-01 v1
  • Checksum
    3AFB5F940242952A
MAWASSFDAFFKNFKRESKIISEYDITLIMTYIEENKLQKAVSVIEKVLRDIESAPLHIAVTGETGAGKSTFINTLRGVGHEEKGAAPTGAIETTMKRTPYPHPKLPNVTIWDLPGIGTTNFTPQNYLTEMKFGEYDFFIIISATRFKENDAQLAKAIAQMGMNFYFVRTKIDSDLDNEQKFKPKSFNKEEVLKNIKDYCSNHLQESLDSEPPVFLVSNVDISKYDFPKLETKLLQDLPAHKRHVFSLSLQSLTEATINYKRDSLKQKVFLEAMKAGALATIPLGGMISDILENLDETFNLYRSYFGLDDASLENIAQDLNMSVDDFKVHLRFPHLFAEHNDESLEDKLFKYIKHISSVTGGPVAAVTYYRMAYYLQNLFLDTAANDAIALLNSKALFEKKVGPYISEPPEYWEA

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict91in Ref. 1; AAA66220
Sequence conflict272in Ref. 2; BAC40974

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U15636
EMBL· GenBank· DDBJ
AAA66220.1
EMBL· GenBank· DDBJ
mRNA
AK089836
EMBL· GenBank· DDBJ
BAC40974.1
EMBL· GenBank· DDBJ
mRNA
AK163978
EMBL· GenBank· DDBJ
BAE37565.1
EMBL· GenBank· DDBJ
mRNA
AK172473
EMBL· GenBank· DDBJ
BAE43026.1
EMBL· GenBank· DDBJ
mRNA
AL627237
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CH466575
EMBL· GenBank· DDBJ
EDL33781.1
EMBL· GenBank· DDBJ
Genomic DNA
BC085259
EMBL· GenBank· DDBJ
AAH85259.1
EMBL· GenBank· DDBJ
mRNA
FR734025
EMBL· GenBank· DDBJ
CBY65988.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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