Q62132 · PTPRR_MOUSE
- ProteinReceptor-type tyrosine-protein phosphatase R
- GenePtprr
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids656 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus. Isoform gamma may have a role in patterning and cellular proliferation of skeletal elements in the precartilaginous/cartilaginous skeleton.
Catalytic activity
- H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 553 | substrate | ||||
Sequence: D | ||||||
Active site | 587 | Phosphocysteine intermediate | ||||
Sequence: C | ||||||
Binding site | 587-593 | substrate | ||||
Sequence: CSAGIGR | ||||||
Binding site | 631 | substrate | ||||
Sequence: Q |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cell junction | |
Cellular Component | cytosol | |
Cellular Component | plasma membrane | |
Molecular Function | protein kinase binding | |
Molecular Function | protein tyrosine phosphatase activity | |
Biological Process | ERBB2 signaling pathway | |
Biological Process | in utero embryonic development | |
Biological Process | negative regulation of epithelial cell migration | |
Biological Process | negative regulation of ERK1 and ERK2 cascade | |
Biological Process | signal transduction |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameReceptor-type tyrosine-protein phosphatase R
- EC number
- Short namesR-PTP-R
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ62132
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Isoform Alpha
Cell membrane ; Single-pass type I membrane protein
Isoform Beta
Note: Locates to the areas within the cytoplasm.
Isoform Gamma
Note: Locates to the areas within the cytoplasm.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 24-226 | Extracellular | ||||
Sequence: RNNDHFLAIRQKKSWKPVFIYDHSQDIKKSLDIAQEAYKHNYHSPSEVQISKHHQIINSAFPRPAYDPSLNLLAESDQDLEIENLPIPAANVIVVTLQMDITKLNITLLRIFRQGVAAALGLLPQQVHINRLIEKKNQVELFVSPGNRKPGETQALQAEEVLRSLNVDGLHQSLPQFGITDVAPEKNVLQGQHEADKIWSKEG | ||||||
Transmembrane | 227-247 | Helical | ||||
Sequence: FYAVVIFLSIFIIIVTCLMII | ||||||
Topological domain | 248-656 | Cytoplasmic | ||||
Sequence: YRLKERLQLSLRQDKEKNQEIHLSPIARQQAQSEAKTTHSMVQPDQAPKVLNVVVDPQGQCTPEIRNSTSTSVCPSPFRMKPIGLQERRGSNVSLTLDMSSLGSVEPFVAVSTPREKVAMEYLQSASRVLTRSQLRDVVASSHLLQSEFMEIPMNFVDPKEIDIPRHGTKNRYKTILPNPLSRVCLRPKNITDSLSTYINANYIRGYSGKEKAFIATQGPMINTVNDFWQMVWQEDSPVIVMITKLKEKNEKCVLYWPEKRGIYGKVEVLVTGVTECDNYTIRNLVLKQGSHTQHVKHYWYTSWPDHKTPDSAQPLLQLMLDVEEDRLASEGRGPVVVHCSAGIGRTGCFIATSIGCQQLKEEGVVDALSIVCQLRVDRGGMVQTSEQYEFVHHALCLFESRLSPETVE |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 338 | Loss of phosphorylation by PKA, constitutive MAPK binding. | ||||
Sequence: S → A | ||||||
Mutagenesis | 338 | Mimics phosphorylation by PKA, prevents MAPK binding. | ||||
Sequence: S → E | ||||||
Mutagenesis | 587 | Loss of phosphatase activity. | ||||
Sequence: C → S | ||||||
Mutagenesis | 593 | Loss of phosphatase activity. | ||||
Sequence: R → M |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 31 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for signal, glycosylation, chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-23 | |||||
Sequence: MRRAVGFPALCLLLNLHAAGCFS | ||||||
Glycosylation | 23 | O-linked (Xyl...) (chondroitin sulfate) serine | ||||
Sequence: S | ||||||
Chain | PRO_0000025460 | 24-656 | Receptor-type tyrosine-protein phosphatase R | |||
Sequence: RNNDHFLAIRQKKSWKPVFIYDHSQDIKKSLDIAQEAYKHNYHSPSEVQISKHHQIINSAFPRPAYDPSLNLLAESDQDLEIENLPIPAANVIVVTLQMDITKLNITLLRIFRQGVAAALGLLPQQVHINRLIEKKNQVELFVSPGNRKPGETQALQAEEVLRSLNVDGLHQSLPQFGITDVAPEKNVLQGQHEADKIWSKEGFYAVVIFLSIFIIIVTCLMIIYRLKERLQLSLRQDKEKNQEIHLSPIARQQAQSEAKTTHSMVQPDQAPKVLNVVVDPQGQCTPEIRNSTSTSVCPSPFRMKPIGLQERRGSNVSLTLDMSSLGSVEPFVAVSTPREKVAMEYLQSASRVLTRSQLRDVVASSHLLQSEFMEIPMNFVDPKEIDIPRHGTKNRYKTILPNPLSRVCLRPKNITDSLSTYINANYIRGYSGKEKAFIATQGPMINTVNDFWQMVWQEDSPVIVMITKLKEKNEKCVLYWPEKRGIYGKVEVLVTGVTECDNYTIRNLVLKQGSHTQHVKHYWYTSWPDHKTPDSAQPLLQLMLDVEEDRLASEGRGPVVVHCSAGIGRTGCFIATSIGCQQLKEEGVVDALSIVCQLRVDRGGMVQTSEQYEFVHHALCLFESRLSPETVE | ||||||
Glycosylation | 128 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Modified residue | 271 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 338 | Phosphoserine; by PKA | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in the heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. Isoform alpha is expressed throughout the granular layer of the cerebellar but not within the Purkinje cells, also in the villi of the ileum and jejunum and both the villi and crypts of the duodenum. Isoform beta is expressed only in the Purkinje cells. Isoform gamma is expressed throughout the brain, the villi and crypts of the duodenum, jejunum and ileum and expressed at low levels in the proximal colon.
Developmental stage
Isoform gamma is the only family member developmentally expressed. Expressed throughout the brain in 15.5 day embryos and in cranial nerve cells, skeletal tissues such as neural crest-derived face bones, and the periphery of cartilaginous skeletal elements including the rib and vertebrae anlage. On day 17.5, expression was observed throughout the brain, trigeminal ganglion, cranofacial bones, oral-facial structures, cervical vertebrae, axis and the ileum. Expression continued in the vertebral column throughout ossification.
Gene expression databases
Interaction
Subunit
Interacts with MAPKs.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q62132 | Mapk1 P63085 | 5 | EBI-6954051, EBI-397697 | |
XENO | Q62132 | MAPK3 P27361 | 3 | EBI-6954051, EBI-73995 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 269-289 | Disordered | ||||
Sequence: HLSPIARQQAQSEAKTTHSMV | ||||||
Compositional bias | 270-289 | Polar residues | ||||
Sequence: LSPIARQQAQSEAKTTHSMV | ||||||
Domain | 392-646 | Tyrosine-protein phosphatase | ||||
Sequence: LQSEFMEIPMNFVDPKEIDIPRHGTKNRYKTILPNPLSRVCLRPKNITDSLSTYINANYIRGYSGKEKAFIATQGPMINTVNDFWQMVWQEDSPVIVMITKLKEKNEKCVLYWPEKRGIYGKVEVLVTGVTECDNYTIRNLVLKQGSHTQHVKHYWYTSWPDHKTPDSAQPLLQLMLDVEEDRLASEGRGPVVVHCSAGIGRTGCFIATSIGCQQLKEEGVVDALSIVCQLRVDRGGMVQTSEQYEFVHHALCLF |
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 7 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 3 isoforms produced by Alternative splicing.
Q62132-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameAlpha
- SynonymsPTPBR7
- Length656
- Mass (Da)74,036
- Last updated1996-11-01 v1
- Checksum31FA82F582992720
Q62132-2
- NameBeta
- SynonymsPTP-SL
Q62132-3
- NameGamma
- Differences from canonical
- 1-244: Missing
Sequence caution
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_005159 | 1-107 | in isoform Beta | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_005161 | 1-244 | in isoform Gamma | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_005160 | 108-121 | in isoform Beta | |||
Sequence: LPIPAANVIVVTLQ → MHRNTRSVSTPTLQ | ||||||
Compositional bias | 270-289 | Polar residues | ||||
Sequence: LSPIARQQAQSEAKTTHSMV |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D31898 EMBL· GenBank· DDBJ | BAA06696.1 EMBL· GenBank· DDBJ | mRNA | ||
Z30313 EMBL· GenBank· DDBJ | CAA82957.1 EMBL· GenBank· DDBJ | mRNA | ||
Z30313 EMBL· GenBank· DDBJ | CAA82958.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AF129509 EMBL· GenBank· DDBJ | AAD29673.1 EMBL· GenBank· DDBJ | mRNA | ||
AF041866 EMBL· GenBank· DDBJ | AAD09171.1 EMBL· GenBank· DDBJ | mRNA |