Q61286 · HTF4_MOUSE
- ProteinTranscription factor 12
- GeneTcf12
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids706 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3') (PubMed:18214987).
May be involved in the functional network that regulates the development of the GnRH axis (By similarity).
May be involved in the functional network that regulates the development of the GnRH axis (By similarity).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTranscription factor 12
- Short namesTCF-12
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ61286
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Mice are smaller than their wild-type littermates and fail to thrive 14 days after birth.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 30 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000127230 | 1-706 | Transcription factor 12 | |||
Sequence: MNPQQQRMAAIGTDKELSDLLDFSAMFSPPVNSGKTRPTTLGSSQFSGSGMDERGGTTSWGTSGQPSPSYDSSRGFTDSPHYSDHLNDSRLGTHEGLSPTPFMNSNLIGKTSERGSFSLYSRDSGLSGCQSSLLRQDLGLGSPAQLSSSGKPGTPYYSFSATSSRRRPLHDSVALDPLQAKKVRKVPPGLPSSVYAPSPNSDDFNRESPSYPSPKPPTSMFASTFFMQDGTHSSSDLWSSSNGMSQPGFGGILGTSTSHMSQSSSYGSLHSHDRLSYPPHSVSPTDINTSLPPMSSFHRGSTSSSPYVAASHTPPINGSDSILGTRGNAAGSSQTGDALGKALASIYSPDHTSSSFPSNPSTPVGSPSPLTGTSQWPRAGGQAPSSPSYENSLHSLKNRVEQQLHEHLQDAMSFLKDVCEQSRMEDRLDRLDDAIHVLRNHAVGPSTSLPTSHSDIHSLLGPSHNASIGNLNSNYGGSSLVTNSRSASMVGTHREDSVSLNGNHSVLSSTVAASNTELNHKTPENFRGGVQNQSGSVVPTEIKTENKEKDENLHEPPSSDDMKSDDESSQKDIKVSSRGRTSSTNEDEDLNPEQKIEREKERRMANNARERLRVRDINEAFKELGRMCQLHLKSEKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSAASAEPPNTLPGAHPGLSESTNPMGHL | ||||||
Modified residue | 47 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 67 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 79 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 98 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 110 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Modified residue | 116 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 124 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 181 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Modified residue | 313 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 333 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 392 | In isoform Q61286-2; Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 543 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Modified residue | 564 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 574 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Modified residue | 581 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 582 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 583 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 633 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 677 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Widely expressed.
Developmental stage
Abundantly expressed during development of the central nervous system, in particular in proliferating neuroblasts and in cells at the initial stages of differentiation. Also expressed at high levels in morphogenetically active regions such as limb buds, somites and mesonephric tubules. Expression decreases once cellular differentiation is over.
Gene expression databases
Interaction
Subunit
Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin, E12 and ITF2 proteins and RUNX1T1 (By similarity).
Interacts with PTF1A. Interacts with NEUROD2. Interacts with BHLHA9
Interacts with PTF1A. Interacts with NEUROD2. Interacts with BHLHA9
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q61286 | Tal1 P22091 | 2 | EBI-8006499, EBI-8006437 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias, motif, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 25-109 | Disordered | ||||
Sequence: AMFSPPVNSGKTRPTTLGSSQFSGSGMDERGGTTSWGTSGQPSPSYDSSRGFTDSPHYSDHLNDSRLGTHEGLSPTPFMNSNLIG | ||||||
Compositional bias | 29-80 | Polar residues | ||||
Sequence: PPVNSGKTRPTTLGSSQFSGSGMDERGGTTSWGTSGQPSPSYDSSRGFTDSP | ||||||
Region | 119-140 | Leucine-zipper | ||||
Sequence: LYSRDSGLSGCQSSLLRQDLGL | ||||||
Compositional bias | 140-167 | Polar residues | ||||
Sequence: LGSPAQLSSSGKPGTPYYSFSATSSRRR | ||||||
Region | 140-222 | Disordered | ||||
Sequence: LGSPAQLSSSGKPGTPYYSFSATSSRRRPLHDSVALDPLQAKKVRKVPPGLPSSVYAPSPNSDDFNRESPSYPSPKPPTSMFA | ||||||
Motif | 181-188 | Nuclear localization signal | ||||
Sequence: KKVRKVPP | ||||||
Compositional bias | 249-271 | Polar residues | ||||
Sequence: FGGILGTSTSHMSQSSSYGSLHS | ||||||
Region | 249-313 | Disordered | ||||
Sequence: FGGILGTSTSHMSQSSSYGSLHSHDRLSYPPHSVSPTDINTSLPPMSSFHRGSTSSSPYVAASHT | ||||||
Compositional bias | 281-313 | Polar residues | ||||
Sequence: SVSPTDINTSLPPMSSFHRGSTSSSPYVAASHT | ||||||
Region | 349-392 | Disordered | ||||
Sequence: PDHTSSSFPSNPSTPVGSPSPLTGTSQWPRAGGQAPSSPSYENS | ||||||
Compositional bias | 520-541 | Polar residues | ||||
Sequence: HKTPENFRGGVQNQSGSVVPTE | ||||||
Region | 520-604 | Disordered | ||||
Sequence: HKTPENFRGGVQNQSGSVVPTEIKTENKEKDENLHEPPSSDDMKSDDESSQKDIKVSSRGRTSSTNEDEDLNPEQKIEREKERRM | ||||||
Compositional bias | 542-574 | Basic and acidic residues | ||||
Sequence: IKTENKEKDENLHEPPSSDDMKSDDESSQKDIK | ||||||
Compositional bias | 581-604 | Basic and acidic residues | ||||
Sequence: TSSTNEDEDLNPEQKIEREKERRM | ||||||
Domain | 601-654 | bHLH | ||||
Sequence: ERRMANNARERLRVRDINEAFKELGRMCQLHLKSEKPQTKLLILHQAVAVILSL | ||||||
Region | 656-679 | Class A specific domain | ||||
Sequence: QQVRERNLNPKAACLKRREEEKVS | ||||||
Region | 674-706 | Disordered | ||||
Sequence: EEEKVSAASAEPPNTLPGAHPGLSESTNPMGHL |
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q61286-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameALF1B
- SynonymsME1A
- Length706
- Mass (Da)75,811
- Last updated2011-07-27 v2
- Checksum4394FE95A297F68C
Q61286-2
- NameALF1A
- SynonymsME1B
- Differences from canonical
- 397-420: Missing
Computationally mapped potential isoform sequences
There are 13 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
V9GX18 | V9GX18_MOUSE | Tcf12 | 127 | ||
V9GXC3 | V9GXC3_MOUSE | Tcf12 | 523 | ||
V9GXG5 | V9GXG5_MOUSE | Tcf12 | 57 | ||
V9GXM8 | V9GXM8_MOUSE | Tcf12 | 57 | ||
V9GXP0 | V9GXP0_MOUSE | Tcf12 | 79 | ||
V9GWS4 | V9GWS4_MOUSE | Tcf12 | 108 | ||
V9GX29 | V9GX29_MOUSE | Tcf12 | 58 | ||
V9GX46 | V9GX46_MOUSE | Tcf12 | 536 | ||
V9GXQ6 | V9GXQ6_MOUSE | Tcf12 | 27 | ||
V9GXR6 | V9GXR6_MOUSE | Tcf12 | 279 | ||
V9GWU2 | V9GWU2_MOUSE | Tcf12 | 666 | ||
V9GX66 | V9GX66_MOUSE | Tcf12 | 21 | ||
Q3UXQ3 | Q3UXQ3_MOUSE | Tcf12 | 477 |
Features
Showing features for compositional bias, alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 29-80 | Polar residues | ||||
Sequence: PPVNSGKTRPTTLGSSQFSGSGMDERGGTTSWGTSGQPSPSYDSSRGFTDSP | ||||||
Compositional bias | 140-167 | Polar residues | ||||
Sequence: LGSPAQLSSSGKPGTPYYSFSATSSRRR | ||||||
Compositional bias | 249-271 | Polar residues | ||||
Sequence: FGGILGTSTSHMSQSSSYGSLHS | ||||||
Compositional bias | 281-313 | Polar residues | ||||
Sequence: SVSPTDINTSLPPMSSFHRGSTSSSPYVAASHT | ||||||
Alternative sequence | VSP_002105 | 397-420 | in isoform ALF1A | |||
Sequence: Missing | ||||||
Sequence conflict | 416 | in Ref. 2; M97635 | ||||
Sequence: K → E | ||||||
Compositional bias | 520-541 | Polar residues | ||||
Sequence: HKTPENFRGGVQNQSGSVVPTE | ||||||
Sequence conflict | 537 | in Ref. 2; M97635/M97636 | ||||
Sequence: V → F | ||||||
Compositional bias | 542-574 | Basic and acidic residues | ||||
Sequence: IKTENKEKDENLHEPPSSDDMKSDDESSQKDIK | ||||||
Compositional bias | 581-604 | Basic and acidic residues | ||||
Sequence: TSSTNEDEDLNPEQKIEREKERRM | ||||||
Sequence conflict | 687 | in Ref. 1; CAA46052 | ||||
Sequence: N → S |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X64840 EMBL· GenBank· DDBJ | CAA46052.1 EMBL· GenBank· DDBJ | mRNA | ||
M97635 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
M97636 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
AC093483 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AC157575 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AC159001 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |