Q60860 · LTC4S_MOUSE
- ProteinLeukotriene C4 synthase
- GeneLtc4s
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids150 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the conjugation of leukotriene A4 with reduced glutathione (GSH) to form leukotriene C4 with high specificity (PubMed:11319240, PubMed:8706658).
Can also catalyze the transfer of a glutathionyl group from glutathione (GSH) to 13(S),14(S)-epoxy-docosahexaenoic acid to form maresin conjugate in tissue regeneration 1 (MCTR1), a bioactive lipid mediator that possess potent anti-inflammatory and proresolving actions (By similarity).
Can also catalyze the transfer of a glutathionyl group from glutathione (GSH) to 13(S),14(S)-epoxy-docosahexaenoic acid to form maresin conjugate in tissue regeneration 1 (MCTR1), a bioactive lipid mediator that possess potent anti-inflammatory and proresolving actions (By similarity).
Catalytic activity
- leukotriene C4 = glutathione + leukotriene A4This reaction proceeds in the backward direction.
- (13S,14S)-epoxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoate + glutathione = (13R)-S-glutathionyl-(14S)-hydroxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoateThis reaction proceeds in the forward direction.
Activity regulation
Inhibited by MK886.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
10.3 μM | leukotriene A4 | |||||
1.9 μM | glutathione |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
2.3 μmol/min/mg | with leukotriene A4 as substrate | ||||
2.2 μmol/min/mg | with glutathione as substrate |
Pathway
Lipid metabolism; leukotriene C4 biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 30 | glutathione (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Active site | 31 | Proton donor | ||||
Sequence: R | ||||||
Binding site | 51-55 | glutathione (UniProtKB | ChEBI) | ||||
Sequence: RAQVN | ||||||
Binding site | 53 | glutathione (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 58-59 | glutathione (UniProtKB | ChEBI) | ||||
Sequence: EY | ||||||
Binding site | 93-97 | glutathione (UniProtKB | ChEBI) | ||||
Sequence: YFQGY | ||||||
Active site | 104 | Proton acceptor | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | nuclear envelope | |
Cellular Component | nuclear membrane | |
Cellular Component | nuclear outer membrane | |
Molecular Function | enzyme activator activity | |
Molecular Function | glutathione binding | |
Molecular Function | glutathione peroxidase activity | |
Molecular Function | glutathione transferase activity | |
Molecular Function | leukotriene-C4 synthase activity | |
Molecular Function | lipid binding | |
Molecular Function | protein-containing complex binding | |
Biological Process | leukotriene biosynthetic process | |
Biological Process | leukotriene metabolic process | |
Biological Process | lipid metabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameLeukotriene C4 synthase
- EC number
- Short namesLTC4 synthase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ60860
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Nucleus outer membrane ; Multi-pass membrane protein
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Nucleus membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-6 | Cytoplasmic | ||||
Sequence: MKDEVA | ||||||
Transmembrane | 7-27 | Helical | ||||
Sequence: LLATVTLVGVLLQAYFSLQVI | ||||||
Topological domain | 28-48 | Lumenal | ||||
Sequence: SARRAFHVSPPLTSGPPEFER | ||||||
Transmembrane | 49-69 | Helical | ||||
Sequence: VFRAQVNCSEYFPLFLATLWV | ||||||
Topological domain | 70-73 | Cytoplasmic | ||||
Sequence: AGIF | ||||||
Transmembrane | 74-94 | Helical | ||||
Sequence: FHEGAAALCGLFYLFARLRYF | ||||||
Topological domain | 95-104 | Lumenal | ||||
Sequence: QGYARSAQLR | ||||||
Transmembrane | 105-124 | Helical | ||||
Sequence: LTPLYASARALWLLVAMAAL | ||||||
Topological domain | 125-150 | Cytoplasmic | ||||
Sequence: GLLVHFLPGTLRTALFRWLQMLLPMA |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Deficient mice developed normally and are fertile. However mice display abnormal inflammatory and hypersensitivity reactions.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 4 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000217749 | 1-150 | Leukotriene C4 synthase | |||
Sequence: MKDEVALLATVTLVGVLLQAYFSLQVISARRAFHVSPPLTSGPPEFERVFRAQVNCSEYFPLFLATLWVAGIFFHEGAAALCGLFYLFARLRYFQGYARSAQLRLTPLYASARALWLLVAMAALGLLVHFLPGTLRTALFRWLQMLLPMA | ||||||
Modified residue | 36 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Phosphorylation at Ser-36 by RPS6KB1 inhibits the leukotriene-C4 synthase activity.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Structure
Sequence
- Sequence statusComplete
- Length150
- Mass (Da)16,814
- Last updated1996-11-01 v1
- Checksum120ABC53892189C0
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q8K355 | Q8K355_MOUSE | Ltc4s | 113 | ||
A0A1Y7VLL2 | A0A1Y7VLL2_MOUSE | Ltc4s | 141 | ||
Q5SVR6 | Q5SVR6_MOUSE | Ltc4s | 178 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U27195 EMBL· GenBank· DDBJ | AAA75042.1 EMBL· GenBank· DDBJ | mRNA | ||
AL627187 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |