Q60358 · MFNA_METJA
- ProteinL-tyrosine/L-aspartate decarboxylase
- GenemfnA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids396 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the decarboxylation of L-tyrosine to produce tyramine for methanofuran biosynthesis (PubMed:15715981).
Can also catalyze the decarboxylation of L-aspartate to produce beta-alanine for coenzyme A (CoA) biosynthesis (PubMed:24891443).
Can also catalyze the decarboxylation of L-aspartate to produce beta-alanine for coenzyme A (CoA) biosynthesis (PubMed:24891443).
Catalytic activity
- L-tyrosine + H+ = tyramine + CO2
Cofactor
Activity regulation
Inhibited by hydroxylamine and O-methylhydroxylamine.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
1.6 mM | L-tyrosine | |||||
0.8 mM | L-aspartate |
pH Dependence
Optimum pH is 7.5-8.5 for tyrosine decarboxylase activity.
Temperature Dependence
Thermostable. Retains full tyrosine decarboxylase activity after heating at 100 degrees Celsius for 10 minutes and 42% of its activity after 10 minutes at 110 degrees Celsius. Inactive after 10 minutes at 121 degrees Celsius.
Pathway
Cofactor biosynthesis; methanofuran biosynthesis.
Cofactor biosynthesis; coenzyme A biosynthesis.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | aspartate 1-decarboxylase activity | |
Molecular Function | pyridoxal phosphate binding | |
Molecular Function | tyrosine decarboxylase activity | |
Biological Process | carboxylic acid metabolic process | |
Biological Process | coenzyme A biosynthetic process | |
Biological Process | methanofuran biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameL-tyrosine/L-aspartate decarboxylase
- EC number
- Short namesTDC/ADC
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Methanomada group > Methanococci > Methanococcales > Methanocaldococcaceae > Methanocaldococcus
Accessions
- Primary accessionQ60358
Proteomes
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000147023 | 1-396 | L-tyrosine/L-aspartate decarboxylase | ||
Modified residue | 245 | N6-(pyridoxal phosphate)lysine | |||
Proteomic databases
Interaction
Structure
Family & Domains
Sequence similarities
Belongs to the group II decarboxylase family. MfnA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length396
- Mass (Da)45,050
- Last updated1997-11-01 v1
- Checksum41CB8DEE45A8BBC0
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L77117 EMBL· GenBank· DDBJ | AAB98031.1 EMBL· GenBank· DDBJ | Genomic DNA |