Q5ZT21 · MAVE_LEGPH
- ProteinEffector protein MavE
- GenemavE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids208 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Virulence effector that is indispensable for endoplasmic reticulum (ER)-mediated remodeling of the Legionella pneumophila-containing vacuole (LCV) and lysosomal evasion (PubMed:33563829).
Essential for intracellular replication in human monocyte-derived macrophages (hMDMs) and amoebae, as well as for intrapulmonary proliferation in mice (PubMed:33563829).
Essential for intracellular replication in human monocyte-derived macrophages (hMDMs) and amoebae, as well as for intrapulmonary proliferation in mice (PubMed:33563829).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Cellular Component | host cell membrane | |
Cellular Component | pathogen-containing vacuole membrane |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameEffector protein MavE
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Legionellales > Legionellaceae > Legionella
Accessions
- Primary accessionQ5ZT21
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Host vacuole, host pathogen-containing vacuole, host pathogen-containing vacuole membrane ; Single-pass membrane protein
Note: Translocated into the host cell via the type IV secretion system (T4SS) (Probable). During infection, localizes to the poles of the LCV membrane (PubMed:33563829).
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 184-204 | Helical | |||
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
The null mutant fails to remodel the LCV with endoplasmic reticulum (ER)-derived vesicles and is trafficked to the lysosomes where it is degraded (PubMed:33563829).
Mutant is defective for intracellular growth in hMDMs, amoeba and for intrapulmonary proliferation in mice (PubMed:33563829).
Mutant is defective for intracellular growth in hMDMs, amoeba and for intrapulmonary proliferation in mice (PubMed:33563829).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 64 | Results in attenuation of L.pneumophila in both hMDMs and amoeba. Vacuoles cannot be remodeled by the ER and are fused to the lysosomes, leading to bacterial degradation. | |||
Mutagenesis | 68 | Results in attenuation of L.pneumophila in amoeba. | |||
Mutagenesis | 77 | Does not significantly impede L.pneumophila replication within hMDMs. | |||
Mutagenesis | 78 | Results in attenuation of L.pneumophila in both hMDMs and amoeba. Vacuoles cannot be remodeled by the ER and are fused to the lysosomes, leading to bacterial degradation. | |||
Mutagenesis | 80 | Does not significantly impede L.pneumophila replication within hMDMs. | |||
Mutagenesis | 102 | Does not show attenuation. | |||
Miscellaneous
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000456174 | 1-208 | Effector protein MavE | ||
Proteomic databases
Interaction
Subunit
Homotrimer.
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Motif | 77-80 | NPxY eukaryotic motif | |||
Domain
Contains a C-terminal transmembrane helix, three copies of tyrosine-based sorting motifs, and an NPxY eukaryotic motif, which binds phosphotyrosine-binding domains present on signaling and adapter eukaryotic proteins (PubMed:33563829).
The NPxY eukaryotic motif is essential for ER-mediated remodeling and lysosomal evasion by the LCV (PubMed:33563829).
The NPxY eukaryotic motif is essential for ER-mediated remodeling and lysosomal evasion by the LCV (PubMed:33563829).
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length208
- Mass (Da)23,928
- Last updated2004-11-23 v1
- ChecksumCF8C3895A42D7952
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE017354 EMBL· GenBank· DDBJ | AAU28406.1 EMBL· GenBank· DDBJ | Genomic DNA |