Q5ZT21 · MAVE_LEGPH

Function

function

Virulence effector that is indispensable for endoplasmic reticulum (ER)-mediated remodeling of the Legionella pneumophila-containing vacuole (LCV) and lysosomal evasion (PubMed:33563829).
Essential for intracellular replication in human monocyte-derived macrophages (hMDMs) and amoebae, as well as for intrapulmonary proliferation in mice (PubMed:33563829).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Cellular Componenthost cell membrane
Cellular Componentpathogen-containing vacuole membrane

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Effector protein MavE

Gene names

    • Name
      mavE
    • Ordered locus names
      lpg2344

Organism names

Accessions

  • Primary accession
    Q5ZT21

Proteomes

Subcellular Location

Secreted
Host vacuole, host pathogen-containing vacuole, host pathogen-containing vacuole membrane
; Single-pass membrane protein
Note: Translocated into the host cell via the type IV secretion system (T4SS) (Probable). During infection, localizes to the poles of the LCV membrane (PubMed:33563829).

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane184-204Helical

Keywords

Phenotypes & Variants

Disruption phenotype

The null mutant fails to remodel the LCV with endoplasmic reticulum (ER)-derived vesicles and is trafficked to the lysosomes where it is degraded (PubMed:33563829).
Mutant is defective for intracellular growth in hMDMs, amoeba and for intrapulmonary proliferation in mice (PubMed:33563829).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis64Results in attenuation of L.pneumophila in both hMDMs and amoeba. Vacuoles cannot be remodeled by the ER and are fused to the lysosomes, leading to bacterial degradation.
Mutagenesis68Results in attenuation of L.pneumophila in amoeba.
Mutagenesis77Does not significantly impede L.pneumophila replication within hMDMs.
Mutagenesis78Results in attenuation of L.pneumophila in both hMDMs and amoeba. Vacuoles cannot be remodeled by the ER and are fused to the lysosomes, leading to bacterial degradation.
Mutagenesis80Does not significantly impede L.pneumophila replication within hMDMs.
Mutagenesis102Does not show attenuation.

Miscellaneous

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004561741-208Effector protein MavE

Proteomic databases

Interaction

Subunit

Homotrimer.

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif77-80NPxY eukaryotic motif

Domain

Contains a C-terminal transmembrane helix, three copies of tyrosine-based sorting motifs, and an NPxY eukaryotic motif, which binds phosphotyrosine-binding domains present on signaling and adapter eukaryotic proteins (PubMed:33563829).
The NPxY eukaryotic motif is essential for ER-mediated remodeling and lysosomal evasion by the LCV (PubMed:33563829).

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    208
  • Mass (Da)
    23,928
  • Last updated
    2004-11-23 v1
  • Checksum
    CF8C3895A42D7952
MTRFIMLSFVTGYRKSISCNRFSRNVVSPYFFMRTIIMTRFERNFLINSLMFLETILSVDKKLDDAIHHFTQGQYENPRYQINSRITNADDWSKEDKLKFTSAIAEAIALVSEKYENPTSETTEQIQSARNILLDNYVPLLTANTDPENRLKSVRENSSQIRKELIAKLKDEVPYKSQFENPYVLFPFVAATVAVAATAASVLFGNKP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE017354
EMBL· GenBank· DDBJ
AAU28406.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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