Essential maintenance is planned to begin on Tue Jan 28 2025. The website may be temporarily unavailable. Please use our fallback: https://wwwdev.ebi.ac.uk/uniprot/front-end/fallback/ during this time.

Q5ZPJ7 · VKT_NAJAT

  • Protein
    Kunitz-type serine protease inhibitor NACI
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Serine protease inhibitor that inhibits chymotrypsin (Ki=25 nM) (PubMed:14990218, PubMed:15698956).
Also interacts with vasopressin V2 receptor (V2R/AVPR2) (PubMed:35122240).
Inhibits vasopressin binding human V2R in the nanomolar range (Ki=112 nM), and also moderately inhibits vasopressin-induced cAMP production (IC50=138 nM). In vivo, intraperitoneal injection of this protein into rats increases diuresis by 2.2-fold, without any loss of electrolytes (PubMed:35122240).

Features

Showing features for site.

1811020304050607080MSSGGLLLLLGLLTLWAELTPVSGRPRFCELAPSAGSCFAFVPSYYYNQYSNTCHSFTYSGCGGNANRFRTIDECNRTCVG
TypeIDPosition(s)Description
Site39-40Reactive bond for chymotrypsin

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Molecular Functionserine-type endopeptidase inhibitor activity
Molecular Functiontoxin activity
Biological Processnegative regulation of peptidase activity

Keywords

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Kunitz-type serine protease inhibitor NACI
  • Alternative names
    • Naja atra chymotrypsin inhibitor (ACI; NA-CI
      )

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Lepidosauria > Squamata > Bifurcata > Unidentata > Episquamata > Toxicofera > Serpentes > Colubroidea > Elapidae > Elapinae > Naja

Accessions

  • Primary accession
    Q5ZPJ7

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for signal, chain, disulfide bond, glycosylation.

Type
IDPosition(s)Description
Signal1-24
ChainPRO_500007217325-81Kunitz-type serine protease inhibitor NACI
Disulfide bond29↔79
Disulfide bond38↔62
Disulfide bond54↔75
Glycosylation76N-linked (GlcNAc...) asparagine

Keywords

Expression

Tissue specificity

Expressed by the venom gland.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain29-79BPTI/Kunitz inhibitor

Sequence similarities

Belongs to the venom Kunitz-type family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    81
  • Mass (Da)
    8,815
  • Last updated
    2004-11-23 v1
  • MD5 Checksum
    0968C640182F85002189046FDE67F1BE
MSSGGLLLLLGLLTLWAELTPVSGRPRFCELAPSAGSCFAFVPSYYYNQYSNTCHSFTYSGCGGNANRFRTIDECNRTCVG

Mass Spectrometry

Molecular mass is 6,403.8 Da. Determined by MALDI.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AJ586046
EMBL· GenBank· DDBJ
CAE51866.1
EMBL· GenBank· DDBJ
mRNA
AJ586047
EMBL· GenBank· DDBJ
CAE51867.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help