Q5ZJM7 · DCK2_CHICK
- ProteinDeoxycytidine kinase 2
- GeneDCK2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids265 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Phosphorylates the deoxyribonucleosides deoxyadenosine, deoxycytidine and deoxyguanosine (PubMed:27906638).
Shows highest activity against deoxyguanosine followed by deoxycytidine and then deoxyadenosine (PubMed:27906638).
Shows only very minor activity against deoxyuridine and deoxythymidine (PubMed:27906638).
Shows highest activity against deoxyguanosine followed by deoxycytidine and then deoxyadenosine (PubMed:27906638).
Shows only very minor activity against deoxyuridine and deoxythymidine (PubMed:27906638).
Catalytic activity
- 2'-deoxycytidine + a ribonucleoside 5'-triphosphate = a ribonucleoside 5'-diphosphate + dCMP + H+
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
19.8 μM | deoxyguanosine | |||||
64 μM | deoxycytidine | |||||
104 μM | deoxyadenosine | |||||
20317 μM | deoxyuridine | |||||
33613 μM | deoxythymidine |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
1.3 μmol/min/mg | toward deoxyguanosine | ||||
1 μmol/min/mg | toward deoxycytidine | ||||
4.9 μmol/min/mg | toward deoxyadenosine | ||||
1 μmol/min/mg | toward deoxyuridine | ||||
0.5 μmol/min/mg | toward deoxythymidine |
kcat is 0.67 sec-1 with deoxyguanosine as substrate. kcat is 0.52 sec-1 with deoxycytidine as substrate. kcat is 2.56 sec-1 with deoxyadenosine as substrate. kcat is 0.53 sec-1 with deoxyuridine as substrate. kcat is 0.26 sec-1 with deoxythymidine as substrate.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 31-39 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GNIAAGKST | ||||||
Binding site | 56 | substrate | ||||
Sequence: E | ||||||
Binding site | 89 | substrate | ||||
Sequence: Y | ||||||
Binding site | 100 | substrate | ||||
Sequence: Q | ||||||
Active site | 130 | Proton acceptor | ||||
Sequence: E | ||||||
Binding site | 131 | substrate | ||||
Sequence: R | ||||||
Binding site | 136 | substrate | ||||
Sequence: D | ||||||
Binding site | 191-195 | ATP (UniProtKB | ChEBI) | ||||
Sequence: RLQKR | ||||||
Binding site | 200 | substrate | ||||
Sequence: E | ||||||
Binding site | 243-245 | ATP (UniProtKB | ChEBI) | ||||
Sequence: EDF |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | mitochondrion | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | deoxyadenosine kinase activity | |
Molecular Function | deoxycytidine kinase activity | |
Molecular Function | deoxyguanosine kinase activity | |
Biological Process | deoxyribonucleoside monophosphate biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDeoxycytidine kinase 2
- EC number
- Short namesGgdCK2
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Galloanserae > Galliformes > Phasianidae > Phasianinae > Gallus
Accessions
- Primary accessionQ5ZJM7
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000449295 | 1-265 | Deoxycytidine kinase 2 | |||
Sequence: MSAPAKRRCRGPAADLDSSFQKRLRKISIEGNIAAGKSTLVRLLEKHSDEWEVIPEPIAKWCNIQTSEDECKELSTSQKSGGNLLQMLYDKPTRWAYTFQTYACLSRVRAQLKPISAKLHEAEHPVQFFERSVYSDRYVFASNLFESGNINETEWAIYQDWHSWLLNQFQSEIELDGIIYLRTTPQKCMERLQKRGRKEEEGIDLEYLENLHYKHETWLYEKTMRVDFENLKEIPILVLDVNEDFKNDKIKQEYLIDEIKSFLTS |
Proteomic databases
Expression
Tissue specificity
Expressed at high levels in adult intestine, spleen, thymus and testis with lower levels in skeletal muscle and eye. In the embryo, expressed at higher levels until day 10 with lower levels in later stages.
Gene expression databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length265
- Mass (Da)31,240
- Last updated2004-11-23 v1
- Checksum5DBE0E85E1131940
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
EU835758 EMBL· GenBank· DDBJ | ACF41169.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ720407 EMBL· GenBank· DDBJ | CAG32066.1 EMBL· GenBank· DDBJ | mRNA | ||
AADN05000072 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |