Q5ZJM7 · DCK2_CHICK

  • Protein
    Deoxycytidine kinase 2
  • Gene
    DCK2
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Phosphorylates the deoxyribonucleosides deoxyadenosine, deoxycytidine and deoxyguanosine (PubMed:27906638).
Shows highest activity against deoxyguanosine followed by deoxycytidine and then deoxyadenosine (PubMed:27906638).
Shows only very minor activity against deoxyuridine and deoxythymidine (PubMed:27906638).

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
19.8 μMdeoxyguanosine
64 μMdeoxycytidine
104 μMdeoxyadenosine
20317 μMdeoxyuridine
33613 μMdeoxythymidine
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
1.3 μmol/min/mgtoward deoxyguanosine
1 μmol/min/mgtoward deoxycytidine
4.9 μmol/min/mgtoward deoxyadenosine
1 μmol/min/mgtoward deoxyuridine
0.5 μmol/min/mgtoward deoxythymidine
kcat is 0.67 sec-1 with deoxyguanosine as substrate. kcat is 0.52 sec-1 with deoxycytidine as substrate. kcat is 2.56 sec-1 with deoxyadenosine as substrate. kcat is 0.53 sec-1 with deoxyuridine as substrate. kcat is 0.26 sec-1 with deoxythymidine as substrate.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site31-39ATP (UniProtKB | ChEBI)
Binding site56substrate
Binding site89substrate
Binding site100substrate
Active site130Proton acceptor
Binding site131substrate
Binding site136substrate
Binding site191-195ATP (UniProtKB | ChEBI)
Binding site200substrate
Binding site243-245ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentmitochondrion
Cellular Componentnucleus
Molecular FunctionATP binding
Molecular Functiondeoxyadenosine kinase activity
Molecular Functiondeoxycytidine kinase activity
Molecular Functiondeoxyguanosine kinase activity
Biological Processdeoxyribonucleoside monophosphate biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Deoxycytidine kinase 2
  • EC number
  • Short names
    GgdCK2
  • Alternative names
    • Deoxyadenosine kinase 2
      (EC:2.7.1.76
      ) . EC:2.7.1.76 (UniProtKB | ENZYME | Rhea)
    • Deoxyguanosine kinase 2
      (EC:2.7.1.113
      ) . EC:2.7.1.113 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      DCK2
    • Synonyms
      DAK
    • ORF names
      RCJMB04_17b6

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • CB
    • Red jungle fowl
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Galloanserae > Galliformes > Phasianidae > Phasianinae > Gallus

Accessions

  • Primary accession
    Q5ZJM7
  • Secondary accessions
    • F6TYL4

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004492951-265Deoxycytidine kinase 2

Proteomic databases

Expression

Tissue specificity

Expressed at high levels in adult intestine, spleen, thymus and testis with lower levels in skeletal muscle and eye. In the embryo, expressed at higher levels until day 10 with lower levels in later stages.

Gene expression databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the DCK/DGK family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    265
  • Mass (Da)
    31,240
  • Last updated
    2004-11-23 v1
  • Checksum
    5DBE0E85E1131940
MSAPAKRRCRGPAADLDSSFQKRLRKISIEGNIAAGKSTLVRLLEKHSDEWEVIPEPIAKWCNIQTSEDECKELSTSQKSGGNLLQMLYDKPTRWAYTFQTYACLSRVRAQLKPISAKLHEAEHPVQFFERSVYSDRYVFASNLFESGNINETEWAIYQDWHSWLLNQFQSEIELDGIIYLRTTPQKCMERLQKRGRKEEEGIDLEYLENLHYKHETWLYEKTMRVDFENLKEIPILVLDVNEDFKNDKIKQEYLIDEIKSFLTS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
EU835758
EMBL· GenBank· DDBJ
ACF41169.1
EMBL· GenBank· DDBJ
mRNA
AJ720407
EMBL· GenBank· DDBJ
CAG32066.1
EMBL· GenBank· DDBJ
mRNA
AADN05000072
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

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