Q5ZIP3 · DOHH_CHICK
- ProteinDeoxyhypusine hydroxylase
- GeneDOHH
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids299 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Catalyzes the hydroxylation of the N6-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.
Catalytic activity
- [eIF5A protein]-deoxyhypusine + AH2 + O2 = [eIF5A protein]-hypusine + A + H2O
Cofactor
Note: Binds 2 Fe2+ ions per subunit.
Pathway
Protein modification; eIF5A hypusination.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 56 | Fe cation 1 (UniProtKB | ChEBI) | |||
Binding site | 89 | Fe cation 2 (UniProtKB | ChEBI) | |||
Binding site | 90 | Fe cation 2 (UniProtKB | ChEBI) | |||
Binding site | 207 | Fe cation 2 (UniProtKB | ChEBI) | |||
Binding site | 240 | Fe cation 1 (UniProtKB | ChEBI) | |||
Binding site | 241 | Fe cation 1 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | deoxyhypusine monooxygenase activity | |
Molecular Function | iron ion binding | |
Biological Process | peptidyl-lysine modification to peptidyl-hypusine |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDeoxyhypusine hydroxylase
- EC number
- Short namesDOHH
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Galloanserae > Galliformes > Phasianidae > Phasianinae > Gallus
Accessions
- Primary accessionQ5ZIP3
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000248578 | 1-299 | Deoxyhypusine hydroxylase | ||
Proteomic databases
Expression
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for repeat.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Repeat | 54-80 | HEAT-like PBS-type 1 | |||
Repeat | 87-113 | HEAT-like PBS-type 2 | |||
Repeat | 174-200 | HEAT-like PBS-type 3 | |||
Repeat | 205-231 | HEAT-like PBS-type 4 | |||
Repeat | 238-264 | HEAT-like PBS-type 5 | |||
Sequence similarities
Belongs to the deoxyhypusine hydroxylase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length299
- Mass (Da)32,875
- Last updated2004-11-23 v1
- MD5 Checksum550A2529C1BDEFDF8B21E7A87D2766B3
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ720741 EMBL· GenBank· DDBJ | CAG32400.1 EMBL· GenBank· DDBJ | mRNA |