Q5Z5R4 · C7016_ORYSJ

Function

function

Catalyzes three successive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid, a key step in gibberellins (GAs) biosynthesis. GAs, which are involved many processes, including stem elongation, play a central role in plant development.

Catalytic activity

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Pathway

Plant hormone biosynthesis; gibberellin biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site449Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentchloroplast outer membrane
Molecular Functionent-kaur-16-en-19-al oxidase activity
Molecular Functionent-kaur-16-en-19-ol oxidase activity
Molecular Functionent-kaurene oxidase activity
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
Biological Processent-kaurene oxidation to kaurenoic acid
Biological Processgibberellin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ent-kaurene oxidase 2
  • EC number
  • Short names
    OsKO2
  • Alternative names
    • Cytochrome P450 701A6
    • Ent-kaurene oxidase-like 2
      (OsKOL2
      )
    • OsKOS3
    • Protein DWARF 35

Gene names

    • Name
      CYP701A6
    • Synonyms
      D35
    • ORF names
      OSJNBa0062E01.38
    • Ordered locus names
      Os06g0570100
      , LOC_Os06g37364

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Xiushui 11
    • cv. Nipponbare
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa

Accessions

  • Primary accession
    Q5Z5R4
  • Secondary accessions
    • A0A0P0WXW2
    • Q68YV8

Proteomes

Genome annotation databases

Subcellular Location

Membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane3-23Helical

Keywords

Phenotypes & Variants

Disruption phenotype

Semi-dwarf phenotype.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis238In d35; semi-dwarf phenotype.

PTM/Processing

Features

Showing features for chain, modified residue (large scale data).

TypeIDPosition(s)SourceDescription
ChainPRO_00004307331-505UniProtEnt-kaurene oxidase 2
Modified residue (large scale data)350PTMeXchangePhosphothreonine

Proteomic databases

Expression

Tissue specificity

Widely expressed.

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    505
  • Mass (Da)
    57,170
  • Last updated
    2004-11-23 v1
  • Checksum
    AFBE61BAFB59BF0A
MEAFVPGGAGAAAAAVGGFVAAAALAERAGVIAPRKRPNAPPAVPGLPIIGNLHQLKEKKPHQTFAKWAEIYGPIYTIRTGASSVVVLNSTEVAKEAMVAKFSSISTRKLSKALTVLTRDKSMVATSDYCDFHKMVKRYVMSSMLGTSAQKQFRDIRDMMIHNMLSTFHKLVKDDPHAPLIFRDVFKDELFRLSMIQSLGEDVSSVYVDEFGRDISKEEIYNATVTDMMMCAIEVDWRDFFPYLSWVPNKSFETRVFTTETRRTAVMRALIKQQKERIVRGEAKTCYLDFLLAENTLTDEQLMMLVWEALIEAADTTLVTTEWAMYELAKNPDKQERLYQEIREVCGDETVTEEHLPRLPYLNAVFHETLRRHSPVPLIPPRFVHEDTKLAGYDVPAGTEMVINLYGCNMNRKEWESPEEWVPERFAGGRLEVADMYKTMAFGAGRRACAGSLQATHIACAAVARFVQEFGWRLREGDEEKVDTVQLTAYKLHPLHVHLTRRGRM

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict11in Ref. 1; AAT81230
Sequence conflict217in Ref. 1; AAT81230

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY660665
EMBL· GenBank· DDBJ
AAT81230.1
EMBL· GenBank· DDBJ
mRNA
AP005471
EMBL· GenBank· DDBJ
BAD54598.1
EMBL· GenBank· DDBJ
Genomic DNA
AP008212
EMBL· GenBank· DDBJ
BAF19823.1
EMBL· GenBank· DDBJ
Genomic DNA
AP014962
EMBL· GenBank· DDBJ
BAS98310.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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