Q5Z5R4 · C7016_ORYSJ
- ProteinEnt-kaurene oxidase 2
- GeneCYP701A6
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids505 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes three successive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid, a key step in gibberellins (GAs) biosynthesis. GAs, which are involved many processes, including stem elongation, play a central role in plant development.
Catalytic activity
- ent-kaur-16-ene + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = ent-kaur-16-en-19-oate + 4 H+ + 4 H2O + 3 oxidized [NADPH--hemoprotein reductase]
Cofactor
Pathway
Plant hormone biosynthesis; gibberellin biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast outer membrane | |
Molecular Function | ent-kaur-16-en-19-al oxidase activity | |
Molecular Function | ent-kaur-16-en-19-ol oxidase activity | |
Molecular Function | ent-kaurene oxidase activity | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen | |
Biological Process | ent-kaurene oxidation to kaurenoic acid | |
Biological Process | gibberellin biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEnt-kaurene oxidase 2
- EC number
- Short namesOsKO2
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ5Z5R4
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 3-23 | Helical | ||||
Sequence: AFVPGGAGAAAAAVGGFVAAA |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Semi-dwarf phenotype.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 238 | In d35; semi-dwarf phenotype. | ||||
Sequence: R → S |
PTM/Processing
Features
Showing features for chain, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000430733 | 1-505 | UniProt | Ent-kaurene oxidase 2 | |||
Sequence: MEAFVPGGAGAAAAAVGGFVAAAALAERAGVIAPRKRPNAPPAVPGLPIIGNLHQLKEKKPHQTFAKWAEIYGPIYTIRTGASSVVVLNSTEVAKEAMVAKFSSISTRKLSKALTVLTRDKSMVATSDYCDFHKMVKRYVMSSMLGTSAQKQFRDIRDMMIHNMLSTFHKLVKDDPHAPLIFRDVFKDELFRLSMIQSLGEDVSSVYVDEFGRDISKEEIYNATVTDMMMCAIEVDWRDFFPYLSWVPNKSFETRVFTTETRRTAVMRALIKQQKERIVRGEAKTCYLDFLLAENTLTDEQLMMLVWEALIEAADTTLVTTEWAMYELAKNPDKQERLYQEIREVCGDETVTEEHLPRLPYLNAVFHETLRRHSPVPLIPPRFVHEDTKLAGYDVPAGTEMVINLYGCNMNRKEWESPEEWVPERFAGGRLEVADMYKTMAFGAGRRACAGSLQATHIACAAVARFVQEFGWRLREGDEEKVDTVQLTAYKLHPLHVHLTRRGRM | |||||||
Modified residue (large scale data) | 350 | PTMeXchange | Phosphothreonine | ||||
Sequence: T |
Proteomic databases
Expression
Tissue specificity
Widely expressed.
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length505
- Mass (Da)57,170
- Last updated2004-11-23 v1
- ChecksumAFBE61BAFB59BF0A
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 11 | in Ref. 1; AAT81230 | ||||
Sequence: A → V | ||||||
Sequence conflict | 217 | in Ref. 1; AAT81230 | ||||
Sequence: K → R |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY660665 EMBL· GenBank· DDBJ | AAT81230.1 EMBL· GenBank· DDBJ | mRNA | ||
AP005471 EMBL· GenBank· DDBJ | BAD54598.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP008212 EMBL· GenBank· DDBJ | BAF19823.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP014962 EMBL· GenBank· DDBJ | BAS98310.1 EMBL· GenBank· DDBJ | Genomic DNA |