Q5WEX1 · Q5WEX1_SHOC1

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site45L-glutamine (UniProtKB | ChEBI)
Binding site217L-glutamine (UniProtKB | ChEBI)
Binding site219L-glutamine (UniProtKB | ChEBI)
Active site244Nucleophile
Binding site245L-glutamine (UniProtKB | ChEBI)
Binding site248L-glutamine (UniProtKB | ChEBI)
Binding site286L-glutamine (UniProtKB | ChEBI)
Binding site289L-glutamine (UniProtKB | ChEBI)
Active site329
Active site331

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • Ordered locus names
      ABC2554

Organism names

Accessions

  • Primary accession
    Q5WEX1

Proteomes

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain1-131Carbamoyl-phosphate synthase small subunit N-terminal
Region1-168CPSase

Sequence similarities

Belongs to the CarA family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    360
  • Mass (Da)
    39,553
  • Last updated
    2004-11-23 v1
  • Checksum
    C63E62349A176C9A
MKGSIVLETGETFVGNWHGEPKQVEGEIVFFTGMTGYQEVITDPSFKGQIVVFTYPLIGNYGVNESDFESSKPQVEGVIVCEAASDPSHWKMKKSVVDYLSEHRIPYMSGVDTRAIVKRIRQHGDMRAVLTTDSRNANVQETRLLSDKFVVADVSVTEPVVYNATGTTHIAVLDYGCKQSIIRSLVKKGAKVTQLPYNTDFAVLEALKPDGVLLSNGPGNPKQLQALLPDIKKVAETFPTLGICLGHQLLALAYGGDTQKLRFGHRGANQPVLDSCTGRVCMTSQNHSYVVTEESLASSILEPRFYNINDRSIEGLVHPKRPVMSIQFHPEASPGPVDSTEIFDAFLAPLLSKGRDHQYA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP006627
EMBL· GenBank· DDBJ
BAD65089.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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