Q5W262 · PIGJ_SERS3

Function

function

Involved in the biosynthesis of 4-methoxy-2,2'-bipyrrole-5-carbaldehyde (MBC), one of the terminal products involved in the biosynthesis of the red antibiotic prodigiosin (Pig). Catalyzes the decarboxylation on the malonyl group attached to PigH to yield a C2 carbanion of acetyl-S-PigH. Then, the heterocyclic pyrrole group of PigG moves to the PigJ active site Cys-525 to generate a transient pyrrolyl-S-cysteinyl PigJ intermediate (acyl donor) whose pyrrolyl group is captured by the C2 carbanion of acetyl-S-PigH to yield the pyrrolyl-beta-ketoacyl-S-PigH.

Pathway

Antibiotic biosynthesis; prodigiosin biosynthesis.

Features

Showing features for active site, binding site.

1770100200300400500600700
TypeIDPosition(s)Description
Active site525For beta-ketoacyl synthase activity
Active site652For beta-ketoacyl synthase activity
Binding site652substrate
Active site690For beta-ketoacyl synthase activity
Binding site690substrate

GO annotations

AspectTerm
Molecular Functionacyltransferase activity
Biological Processantibiotic biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Beta-ketoacyl synthase PigJ
  • EC number
  • Short names
    PKS

Gene names

    • Name
      pigJ

Organism names

Accessions

  • Primary accession
    Q5W262

Phenotypes & Variants

Disruption phenotype

Cells lacking this gene show a white phenotype and produce 2-methyl-3-n-amyl-pyrrole (MAP).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004362471-770Beta-ketoacyl synthase PigJ

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain342-768Ketosynthase family 3 (KS3)

Domain

It seems that PigJ is composed by an active ketosynthase (KS) domain and by a chain length factor (CLF) partner domain that potentially decarboxylates the malonyl group of PigH.

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    770
  • Mass (Da)
    83,742
  • Last updated
    2004-12-07 v1
  • Checksum
    89C02E13CA936E33
MSNDKHIAPLAVVSMGCVLPGVDHFRALDTIADWETVFQSASPLAWSETSRPIQGRQMDDSGFDFKKFSIPPLFRKAVSRETRLALRAAEDALAGLVLPESLRDCCDQFCAIHLGSDAAYRNATKVGALRALAEKLQAQGCPAAEVRRRLDDYKQPLAESLGCSSHDRVGEMASSIPARIAHFAHTRGKCQTLDGADKGGLRLLQLAQDCFRYHDSQMAVLTSVQCFHHRPQAYMLLEQGVSQDACWLEGAISLVVCPLAVAHEQGWPVLTQLGDIVTTHDGSPQPEADHPAALYFAGANQVFCQIVEMVLRQHQRCEGRSFTGGRWQVNVAQTQSLTPAVDDRVAIVDYQPITGHPLDKTQFWQTLEQGEDALREHSAAHVNAEAFVRTTQQKLSTYIHRTMSFPAHSPSDVALKKPMMPAKKQRLDVTQLYALNSCHSWSEKIRQFERVAIIIASNLSLSADRLQAMRALWSGLPGSEGAIPLPELPSINHWSWYGACGIGTAQLLAQYFGISADCYAVEAACASSLAAVHDAVRALQAGRYDAVIVGGIETATLERDLVLCSAQMMLSVSRIRPFSQGADGFTPGDGGGFVMLTHHPVPRAIATIEAISGSCDSYSMTAPDPLGQALAIKKTLSLTAIDAQTVQYLEAHGTGTELGDRSEVMSLKYSYHRDKHSPLYIGSAKYNFGHCFAGAGALSLCKVLSAFEHERIPPTPVSELNVDLPLGDIPAEVPQQAIPWRLSEDGQRKAAINAFGTGGINYHLVIRQSS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AJ833001
EMBL· GenBank· DDBJ
CAH55638.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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