Q5W262 · PIGJ_SERS3
- ProteinBeta-ketoacyl synthase PigJ
- GenepigJ
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids770 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Involved in the biosynthesis of 4-methoxy-2,2'-bipyrrole-5-carbaldehyde (MBC), one of the terminal products involved in the biosynthesis of the red antibiotic prodigiosin (Pig). Catalyzes the decarboxylation on the malonyl group attached to PigH to yield a C2 carbanion of acetyl-S-PigH. Then, the heterocyclic pyrrole group of PigG moves to the PigJ active site Cys-525 to generate a transient pyrrolyl-S-cysteinyl PigJ intermediate (acyl donor) whose pyrrolyl group is captured by the C2 carbanion of acetyl-S-PigH to yield the pyrrolyl-beta-ketoacyl-S-PigH.
Pathway
Antibiotic biosynthesis; prodigiosin biosynthesis.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 525 | For beta-ketoacyl synthase activity | ||||
Sequence: C | ||||||
Active site | 652 | For beta-ketoacyl synthase activity | ||||
Sequence: H | ||||||
Binding site | 652 | substrate | ||||
Sequence: H | ||||||
Active site | 690 | For beta-ketoacyl synthase activity | ||||
Sequence: H | ||||||
Binding site | 690 | substrate | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | acyltransferase activity | |
Biological Process | antibiotic biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBeta-ketoacyl synthase PigJ
- EC number
- Short namesPKS
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Pectobacteriaceae > Prodigiosinella
Accessions
- Primary accessionQ5W262
Phenotypes & Variants
Disruption phenotype
Cells lacking this gene show a white phenotype and produce 2-methyl-3-n-amyl-pyrrole (MAP).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000436247 | 1-770 | Beta-ketoacyl synthase PigJ | |||
Sequence: MSNDKHIAPLAVVSMGCVLPGVDHFRALDTIADWETVFQSASPLAWSETSRPIQGRQMDDSGFDFKKFSIPPLFRKAVSRETRLALRAAEDALAGLVLPESLRDCCDQFCAIHLGSDAAYRNATKVGALRALAEKLQAQGCPAAEVRRRLDDYKQPLAESLGCSSHDRVGEMASSIPARIAHFAHTRGKCQTLDGADKGGLRLLQLAQDCFRYHDSQMAVLTSVQCFHHRPQAYMLLEQGVSQDACWLEGAISLVVCPLAVAHEQGWPVLTQLGDIVTTHDGSPQPEADHPAALYFAGANQVFCQIVEMVLRQHQRCEGRSFTGGRWQVNVAQTQSLTPAVDDRVAIVDYQPITGHPLDKTQFWQTLEQGEDALREHSAAHVNAEAFVRTTQQKLSTYIHRTMSFPAHSPSDVALKKPMMPAKKQRLDVTQLYALNSCHSWSEKIRQFERVAIIIASNLSLSADRLQAMRALWSGLPGSEGAIPLPELPSINHWSWYGACGIGTAQLLAQYFGISADCYAVEAACASSLAAVHDAVRALQAGRYDAVIVGGIETATLERDLVLCSAQMMLSVSRIRPFSQGADGFTPGDGGGFVMLTHHPVPRAIATIEAISGSCDSYSMTAPDPLGQALAIKKTLSLTAIDAQTVQYLEAHGTGTELGDRSEVMSLKYSYHRDKHSPLYIGSAKYNFGHCFAGAGALSLCKVLSAFEHERIPPTPVSELNVDLPLGDIPAEVPQQAIPWRLSEDGQRKAAINAFGTGGINYHLVIRQSS |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 342-768 | Ketosynthase family 3 (KS3) | ||||
Sequence: DDRVAIVDYQPITGHPLDKTQFWQTLEQGEDALREHSAAHVNAEAFVRTTQQKLSTYIHRTMSFPAHSPSDVALKKPMMPAKKQRLDVTQLYALNSCHSWSEKIRQFERVAIIIASNLSLSADRLQAMRALWSGLPGSEGAIPLPELPSINHWSWYGACGIGTAQLLAQYFGISADCYAVEAACASSLAAVHDAVRALQAGRYDAVIVGGIETATLERDLVLCSAQMMLSVSRIRPFSQGADGFTPGDGGGFVMLTHHPVPRAIATIEAISGSCDSYSMTAPDPLGQALAIKKTLSLTAIDAQTVQYLEAHGTGTELGDRSEVMSLKYSYHRDKHSPLYIGSAKYNFGHCFAGAGALSLCKVLSAFEHERIPPTPVSELNVDLPLGDIPAEVPQQAIPWRLSEDGQRKAAINAFGTGGINYHLVIRQ |
Domain
It seems that PigJ is composed by an active ketosynthase (KS) domain and by a chain length factor (CLF) partner domain that potentially decarboxylates the malonyl group of PigH.
Sequence similarities
Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length770
- Mass (Da)83,742
- Last updated2004-12-07 v1
- Checksum89C02E13CA936E33
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ833001 EMBL· GenBank· DDBJ | CAH55638.1 EMBL· GenBank· DDBJ | Genomic DNA |