Q5VP70 · PIN3A_ORYSJ
- ProteinAuxin efflux carrier component 3a
- GenePIN3A
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids618 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Acts as a component of the auxin efflux carrier. Involved in the polar auxin transport which may regulate crown root development and response to water stress.
Miscellaneous
Seedlings silencing PIN3A display abnormal crown root development. Seedling over-expressing PIN3A exhibit better shoot growth, longer roots and a greater number of adventitious roots than the wild-type when grown on PEG-induced osmotic stress.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 51 | (indol-3-yl)acetic acid (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Binding site | 115 | (indol-3-yl)acetic acid (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 117 | (indol-3-yl)acetic acid (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 148 | (indol-3-yl)acetic acid (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 578 | (indol-3-yl)acetic acid (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 579 | (indol-3-yl)acetic acid (UniProtKB | ChEBI) | ||||
Sequence: V |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | auxin efflux carrier complex | |
Cellular Component | endoplasmic reticulum | |
Cellular Component | plasma membrane | |
Molecular Function | auxin efflux transmembrane transporter activity | |
Molecular Function | identical protein binding | |
Molecular Function | protein homodimerization activity | |
Biological Process | auxin export across the plasma membrane | |
Biological Process | auxin polar transport | |
Biological Process | auxin-activated signaling pathway |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAuxin efflux carrier component 3a
- Short namesOsPIN3a
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ5VP70
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-6 | Extracellular | ||||
Sequence: MISGHD | ||||||
Transmembrane | 7-27 | Helical; Name=1 | ||||
Sequence: FYTVMAAVVPLYVAMFLAYGS | ||||||
Topological domain | 28-38 | Cytoplasmic | ||||
Sequence: VRWWGIFTPDQ | ||||||
Transmembrane | 39-59 | Helical; Name=2 | ||||
Sequence: CSGINRFVAIFAVPLLSFHFI | ||||||
Topological domain | 60-70 | Extracellular | ||||
Sequence: STNDPYAMNLR | ||||||
Transmembrane | 71-90 | Helical; Name=3 | ||||
Sequence: FLAADTLQKLLVLAGLAAWS | ||||||
Topological domain | 91-104 | Cytoplasmic | ||||
Sequence: RLPSRTGAPRLDWS | ||||||
Transmembrane | 105-125 | Helical; Name=4 | ||||
Sequence: ITLFSLSTLPNTLVMGIPLLI | ||||||
Topological domain | 126-134 | Extracellular | ||||
Sequence: AMYGPYSGS | ||||||
Transmembrane | 135-155 | Helical; Name=5 | ||||
Sequence: LMVQIVVLQCIIWYTLMLFLF | ||||||
Topological domain | 156-478 | Cytoplasmic | ||||
Sequence: EFRAARMLIADQFPDTAASIVSLHVDPDVVSLEGGHAETEAEVAADGRLHVTVRRSSVSRRSLLVTPRPSNLTGAEIYSLSSSRNPTPRGSNFNHADFFAMVGGGPPPPTPAAVRGSSFGASELYSLQSSRGPTPRQSNFDEHSARPPKPPATTTGALNHDAKELHMFVWSSSASPVSEVSGLPVFSGGGGGGALDVGAKEIHMVIPADLPQNNGSGKEHEEYGAVALGGGGGGENFSFGGGKTVDGAEAVDEEAALPDGLTKMGSSSTAELHPKVVDVDGPNAGGGAAGAGQYQMPPASVMTRLILIMVWRKLIRNPNTYSS | ||||||
Transmembrane | 479-499 | Helical; Name=6 | ||||
Sequence: LLGLAWSLVAFRWHVSMPAIV | ||||||
Topological domain | 500-502 | Extracellular | ||||
Sequence: EKS | ||||||
Transmembrane | 503-523 | Helical; Name=7 | ||||
Sequence: ISILSDAGLGMAMFSLGLFMA | ||||||
Topological domain | 524-539 | Cytoplasmic | ||||
Sequence: LQPSIIACGKSAAVVS | ||||||
Transmembrane | 540-560 | Helical; Name=8 | ||||
Sequence: MAVRFLAGPAVMAAASIAIGL | ||||||
Topological domain | 561-563 | Extracellular | ||||
Sequence: RGT | ||||||
Transmembrane | 564-584 | Helical; Name=9 | ||||
Sequence: LLHVAIVQAALPQGIVPFVFA | ||||||
Topological domain | 585-597 | Cytoplasmic | ||||
Sequence: KEYNVHPAILSTA | ||||||
Transmembrane | 598-618 | Helical; Name=10 | ||||
Sequence: VIFGMLIALPITLLYYILLGL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000123792 | 1-618 | UniProt | Auxin efflux carrier component 3a | |||
Sequence: MISGHDFYTVMAAVVPLYVAMFLAYGSVRWWGIFTPDQCSGINRFVAIFAVPLLSFHFISTNDPYAMNLRFLAADTLQKLLVLAGLAAWSRLPSRTGAPRLDWSITLFSLSTLPNTLVMGIPLLIAMYGPYSGSLMVQIVVLQCIIWYTLMLFLFEFRAARMLIADQFPDTAASIVSLHVDPDVVSLEGGHAETEAEVAADGRLHVTVRRSSVSRRSLLVTPRPSNLTGAEIYSLSSSRNPTPRGSNFNHADFFAMVGGGPPPPTPAAVRGSSFGASELYSLQSSRGPTPRQSNFDEHSARPPKPPATTTGALNHDAKELHMFVWSSSASPVSEVSGLPVFSGGGGGGALDVGAKEIHMVIPADLPQNNGSGKEHEEYGAVALGGGGGGENFSFGGGKTVDGAEAVDEEAALPDGLTKMGSSSTAELHPKVVDVDGPNAGGGAAGAGQYQMPPASVMTRLILIMVWRKLIRNPNTYSSLLGLAWSLVAFRWHVSMPAIVEKSISILSDAGLGMAMFSLGLFMALQPSIIACGKSAAVVSMAVRFLAGPAVMAAASIAIGLRGTLLHVAIVQAALPQGIVPFVFAKEYNVHPAILSTAVIFGMLIALPITLLYYILLGL | |||||||
Modified residue (large scale data) | 293 | PTMeXchange | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 393 | PTMeXchange | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 421 | PTMeXchange | Phosphoserine | ||||
Sequence: S |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in coleoptiles, roots, vascular bundles of leaves, shoots, lamina joints and vascular bundles of the lemma and filament (PubMed:22882529).
Expressed in stem bases, stems, leaves and young panicles (PubMed:19825657).
Expressed in stem bases, stems, leaves and young panicles (PubMed:19825657).
Induction
By auxin and osmotic stress. Down-regulated by 1-N-naphthylphthalamic acid (NPA), an auxin efflux inhibitor.
Interaction
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 281-296 | Polar residues | ||||
Sequence: SLQSSRGPTPRQSNFD | ||||||
Region | 281-312 | Disordered | ||||
Sequence: SLQSSRGPTPRQSNFDEHSARPPKPPATTTGA |
Sequence similarities
Belongs to the auxin efflux carrier (TC 2.A.69.1) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q5VP70-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length618
- Mass (Da)65,255
- Last updated2004-12-07 v1
- Checksum0E8EDDE44E3BF2EE
Q5VP70-2
- Name2
- Differences from canonical
- 394-422: Missing
Features
Showing features for sequence conflict, compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 154 | in Ref. 6; AK101504 | ||||
Sequence: L → H | ||||||
Compositional bias | 281-296 | Polar residues | ||||
Sequence: SLQSSRGPTPRQSNFD | ||||||
Sequence conflict | 330 | in Ref. 6; AK101504 | ||||
Sequence: S → Y | ||||||
Sequence conflict | 348 | in Ref. 6; AK063976 | ||||
Sequence: Missing | ||||||
Alternative sequence | VSP_017010 | 394-422 | in isoform 2 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP003725 EMBL· GenBank· DDBJ | BAD68753.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP003725 EMBL· GenBank· DDBJ | BAD68754.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP008207 EMBL· GenBank· DDBJ | BAF05606.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP014957 EMBL· GenBank· DDBJ | BAS73380.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP014957 EMBL· GenBank· DDBJ | BAS73381.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CM000138 EMBL· GenBank· DDBJ | EEE55070.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK063976 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
AK101504 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
BR000832 EMBL· GenBank· DDBJ | FAA00681.1 EMBL· GenBank· DDBJ | Genomic DNA |