Q5U3U7 · NLS1A_DANRE
- ProteinSodium-dependent lysophosphatidylcholine symporter 1-A
- Genemfsd2aa
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids532 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Sodium-dependent lysophosphatidylcholine (LPC) symporter, which plays an essential role for blood-brain barrier formation and function. Specifically expressed in endothelium of the blood-brain barrier of micro-vessels and transports LPC into the brain. Transport of LPC is essential because it constitutes the major mechanism by which docosahexaenoic acid (DHA), an omega-3 fatty acid that is essential for normal brain growth and cognitive function, enters the brain. Transports LPC carrying long-chain fatty acids such LPC oleate and LPC palmitate with a minimum acyl chain length of 14 carbons. Does not transport docosahexaenoic acid in unesterified fatty acid.
Catalytic activity
- a 1-acyl-sn-glycero-3-phosphocholine(in) + Na+(in) = a 1-acyl-sn-glycero-3-phosphocholine(out) + Na+(out)
- 1-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine(in) + Na+(in) = 1-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine(out) + Na+(out)
- 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine(in) + Na+(in) = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine(out) + Na+(out)
- 1-hexadecanoyl-sn-glycero-3-phosphocholine(in) + Na+(in) = 1-hexadecanoyl-sn-glycero-3-phosphocholine(out) + Na+(out)
- a 1-acyl-sn-glycero-3-phosphoethanolamine(in) + Na+(in) = a 1-acyl-sn-glycero-3-phosphoethanolamine(out) + Na+(out)
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | plasma membrane | |
Molecular Function | fatty acid transmembrane transporter activity | |
Molecular Function | lysophospholipid:sodium symporter activity | |
Molecular Function | symporter activity | |
Biological Process | carbohydrate transport | |
Biological Process | establishment of blood-brain barrier | |
Biological Process | fatty acid transport | |
Biological Process | lipid transport across blood-brain barrier | |
Biological Process | lysophospholipid translocation | |
Biological Process | lysophospholipid transport | |
Biological Process | maintenance of blood-brain barrier | |
Biological Process | transcytosis |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameSodium-dependent lysophosphatidylcholine symporter 1-A
- Short namesNLS1-A; Sodium-dependent LPC symporter 1-A
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio
Accessions
- Primary accessionQ5U3U7
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-40 | Cytoplasmic | ||||
Sequence: MARGEGAEQFSSGLLPTAKSVTQNEIKMVKLPKQQERKRA | ||||||
Transmembrane | 41-70 | Helical | ||||
Sequence: LTVWSKVCFAIGGAPYQITGTALGFFLQIF | ||||||
Topological domain | 71-81 | Extracellular | ||||
Sequence: LLDVAQLNPLN | ||||||
Transmembrane | 82-102 | Helical | ||||
Sequence: ASVILFVGRAWDAVTDPTVGF | ||||||
Topological domain | 103-114 | Cytoplasmic | ||||
Sequence: LVSRTPWTRHGR | ||||||
Transmembrane | 115-134 | Helical | ||||
Sequence: MMPWILVSTIPAVLCYFLIW | ||||||
Topological domain | 135-144 | Extracellular | ||||
Sequence: VVPPIEQGKM | ||||||
Transmembrane | 145-169 | Helical | ||||
Sequence: MWYLLFYCLFQTLQTCFHVPYSALT | ||||||
Topological domain | 170-176 | Cytoplasmic | ||||
Sequence: MFISTEQ | ||||||
Transmembrane | 177-208 | Helical | ||||
Sequence: RERDSATAYRMTVEVFGTVVGTAIQGQIVGMA | ||||||
Topological domain | 209-232 | Extracellular | ||||
Sequence: NTPCKNNTSPNNSSNDLIQSNNSH | ||||||
Transmembrane | 233-266 | Helical | ||||
Sequence: IPLKSNIFDERCAYMIASAVISLIYVVCAAVLFF | ||||||
Topological domain | 267-297 | Cytoplasmic | ||||
Sequence: GVREQDVQGELKAQKRVSFQKGLRLVMGHGP | ||||||
Transmembrane | 298-324 | Helical | ||||
Sequence: YVKLVLAFLFTSLAFMLLEGNFAVFIK | ||||||
Topological domain | 325-335 | Extracellular | ||||
Sequence: YTLGFREDFQN | ||||||
Transmembrane | 336-354 | Helical | ||||
Sequence: ILLVIMVSATVSIPMWQWF | ||||||
Topological domain | 355-358 | Cytoplasmic | ||||
Sequence: LCRF | ||||||
Transmembrane | 359-380 | Helical | ||||
Sequence: GKKTAVYIGITWAVPFMILVVS | ||||||
Topological domain | 381-383 | Extracellular | ||||
Sequence: VNS | ||||||
Transmembrane | 384-420 | Helical | ||||
Sequence: SLIVSYIVSIAAGVSVGAAFLLPWSMLPDVVDDFKLQ | ||||||
Topological domain | 421-430 | Cytoplasmic | ||||
Sequence: NPTSQGHEAI | ||||||
Transmembrane | 431-457 | Helical | ||||
Sequence: FYSFYVFFTKFASGVSLGVSTLALSFA | ||||||
Topological domain | 458-469 | Extracellular | ||||
Sequence: GYETGVCVQSDS | ||||||
Transmembrane | 470-493 | Helical | ||||
Sequence: VNLTLKLLVSAAPVSLIALGLLIF | ||||||
Topological domain | 494-532 | Cytoplasmic | ||||
Sequence: MTYPIDEERREYNNKQLQLLLRNEEEEDEMEVLKPDITA |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Morpholino knockdown of the gene results in early postnatal lethality and microcephaly.
PTM/Processing
Features
Showing features for chain, disulfide bond, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000273390 | 1-532 | Sodium-dependent lysophosphatidylcholine symporter 1-A | |||
Sequence: MARGEGAEQFSSGLLPTAKSVTQNEIKMVKLPKQQERKRALTVWSKVCFAIGGAPYQITGTALGFFLQIFLLDVAQLNPLNASVILFVGRAWDAVTDPTVGFLVSRTPWTRHGRMMPWILVSTIPAVLCYFLIWVVPPIEQGKMMWYLLFYCLFQTLQTCFHVPYSALTMFISTEQRERDSATAYRMTVEVFGTVVGTAIQGQIVGMANTPCKNNTSPNNSSNDLIQSNNSHIPLKSNIFDERCAYMIASAVISLIYVVCAAVLFFGVREQDVQGELKAQKRVSFQKGLRLVMGHGPYVKLVLAFLFTSLAFMLLEGNFAVFIKYTLGFREDFQNILLVIMVSATVSIPMWQWFLCRFGKKTAVYIGITWAVPFMILVVSVNSSLIVSYIVSIAAGVSVGAAFLLPWSMLPDVVDDFKLQNPTSQGHEAIFYSFYVFFTKFASGVSLGVSTLALSFAGYETGVCVQSDSVNLTLKLLVSAAPVSLIALGLLIFMTYPIDEERREYNNKQLQLLLRNEEEEDEMEVLKPDITA | ||||||
Disulfide bond | 212↔464 | |||||
Sequence: CKNNTSPNNSSNDLIQSNNSHIPLKSNIFDERCAYMIASAVISLIYVVCAAVLFFGVREQDVQGELKAQKRVSFQKGLRLVMGHGPYVKLVLAFLFTSLAFMLLEGNFAVFIKYTLGFREDFQNILLVIMVSATVSIPMWQWFLCRFGKKTAVYIGITWAVPFMILVVSVNSSLIVSYIVSIAAGVSVGAAFLLPWSMLPDVVDDFKLQNPTSQGHEAIFYSFYVFFTKFASGVSLGVSTLALSFAGYETGVC | ||||||
Glycosylation | 214 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 220 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 229 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in the developing nervous system.
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length532
- Mass (Da)59,266
- Last updated2004-12-07 v1
- Checksum387709380163A7F2
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8M2B365 | A0A8M2B365_DANRE | mfsd2aa | 550 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC085388 EMBL· GenBank· DDBJ | AAH85388.1 EMBL· GenBank· DDBJ | mRNA |