Q5U3H2 · KMT5B_DANRE

Function

function

Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity. In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes (By similarity).
H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5B is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (By similarity).
Plays a role in myogenesis by regulating the expression of target genes, such as EID3. Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity).
May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity).

Catalytic activity

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site96S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site178-181S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site185S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site232S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site247-248S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site250Zn2+ (UniProtKB | ChEBI)
Binding site294Zn2+ (UniProtKB | ChEBI)
Binding site295S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site296Zn2+ (UniProtKB | ChEBI)
Binding site299Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchromosome
Cellular Componentnucleus
Molecular Functionchromatin binding
Molecular Functionhistone H4K20 methyltransferase activity
Molecular Functionhistone H4K20 monomethyltransferase activity
Molecular Functionhistone H4K20me methyltransferase activity
Molecular Functionmetal ion binding
Molecular FunctionS-adenosyl-L-methionine binding
Biological ProcessDNA repair
Biological Processmethylation
Biological Processmuscle organ development
Biological Processpositive regulation of double-strand break repair via nonhomologous end joining
Biological Processpositive regulation of isotype switching
Biological Processregulation of multicellular organism growth

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Histone-lysine N-methyltransferase KMT5B
  • Alternative names
    • Lysine-specific methyltransferase 5B
    • Suppressor of variegation 4-20 homolog 1 (Su(var)4-20 homolog 1; Suv4-20h1)
    • [histone H4]-N-methyl-L-lysine20 N-methyltransferase KMT5B
      (EC:2.1.1.362
      ) . EC:2.1.1.362 (UniProtKB | ENZYME | Rhea)
    • [histone H4]-lysine20 N-methyltransferase KMT5B
      (EC:2.1.1.361
      ) . EC:2.1.1.361 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      kmt5b
    • Synonyms
      suv420h1
    • ORF names
      si:dkey-12e7.2, zgc:103527

Organism names

  • Taxonomic identifier
  • Strain
    • Tuebingen
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio

Accessions

  • Primary accession
    Q5U3H2
  • Secondary accessions
    • Q1LWF0

Proteomes

    • Identifier
    • Component
      Alternate scaffold 18, Chromosome 18

Organism-specific databases

Subcellular Location

Chromosome
Note: Associated with pericentric heterochromatin.

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002817901-808Histone-lysine N-methyltransferase KMT5B

Proteomic databases

Expression

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region1-68Disordered
Compositional bias15-46Polar residues
Domain168-283SET
Region339-569Disordered
Compositional bias343-373Polar residues
Compositional bias387-420Polar residues
Compositional bias421-435Basic residues
Compositional bias448-465Basic and acidic residues
Compositional bias466-484Polar residues
Compositional bias491-520Polar residues
Region599-684Disordered

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    808
  • Mass (Da)
    90,438
  • Last updated
    2007-04-03 v2
  • Checksum
    11510B288E3195B0
MGESKNMVLNGRRHGRKFSSNQPVSKSRLQNTQRSHLRQNKGSPSVRRCSRRCGGAPPEAERRHVPSSGMTAKELCEYDDLSTSLILDPYLGFQTHKMNTRFRPIKGRQRELREIIELFKKHDNLEKAFQALTSGDWTRHHFLNKTKSQEKLFKAHVFVYLRMFASDSGFEILSCNRYSSEQNGAKIVATKDWKRNDKIEHLVGCIAELSPSEERMLLRHGENDFSVMYSTRKNCAQLWLGPAAFINHDCRPNCKFVSTGRDTACVKVLRDIEPGEEISCYYGDGFFGENNEFCECYTCERRGTGAFKSKPGLPVEAPVINSKYGLRETDKRLNRLKKLGESCRNSDSQSVSSNAEADSQEPTTVQTSLRKRTSQSCVKKHGEAKAVTRQTLSSTPSSTSSSKRSQANISSLPKRLKSKPTQTLSKGRRRCRGLWTKGSSRVSASGNLKESSRRDTDRRRSASKGSVARSSENNRSSSKGASPCKDSTLCPYRTRRSTRTSLGAQGAEGTEASNHPASPSIVLKSEPGEFIPVTLGHQMSTPSLDSSCPKKGTCPRRRRTVKQEDSYGESFVQEGVPDLRHAVRAADVADCGKVVLGLPDRHQHYNGSSKSSKALRRGKGKKKRQITRYDAQLILQNNSGIPKITLRRRRDSSSSKNEPRETSSSSSSKISIKFSKEHEKDRSSSYVAKLNNGFSHGPHSSSTKLKIQLKREEDPASLHRTYPEDVTLGIVQRDAADVLDHKAAAQVGQDMEVESMSSEDDDDDYFDNEDDFIPLPPAKRLRLIVGKDSIDIDISSRRREDQSLRLNA

Features

Showing features for compositional bias, sequence conflict.

TypeIDPosition(s)Description
Compositional bias15-46Polar residues
Compositional bias343-373Polar residues
Compositional bias387-420Polar residues
Compositional bias421-435Basic residues
Compositional bias448-465Basic and acidic residues
Compositional bias466-484Polar residues
Compositional bias491-520Polar residues
Sequence conflict535in Ref. 2; AAH85544

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BX571884
EMBL· GenBank· DDBJ
CAK05146.1
EMBL· GenBank· DDBJ
Genomic DNA
BC085544
EMBL· GenBank· DDBJ
AAH85544.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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