Q5SMM6 · HCT4_ORYSJ
- ProteinHydroxycinnamoyltransferase 4
- GeneHCT4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids433 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Hydroxycinnamoyl transferase that catalyzes in vitro the transfer of an acyl from p-coumaryol-CoA to glycerol or shikimate, to produce 2-O-p-coumaroyl glyceride or coumaroyl shikimate, respectively. Quercetin, glutarate, or malate do not serve as acyl acceptors in vitro. Can use feruloyl-CoA and caffeoyl-CoA as acyl donors.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
60.1 μM | p-coumaroyl-CoA | |||||
72.1 μM | feruloyl-CoA | |||||
102 μM | caffeoyl-CoA | |||||
85.9 μM | glycerol | |||||
155.8 μM | shikimate |
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 159 | Proton acceptor | ||||
Sequence: H | ||||||
Active site | 375 | Proton acceptor | ||||
Sequence: D |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Molecular Function | acyltransferase activity, transferring groups other than amino-acyl groups | |
Molecular Function | hydroxycinnamoyltransferase activity |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHydroxycinnamoyltransferase 4
- EC number
- Short namesOsHCT4
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ5SMM6
Proteomes
Genome annotation databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000437765 | 1-433 | Hydroxycinnamoyltransferase 4 | |||
Sequence: MATVDVLTSEVVVPAGETPAGAVWLSNLDLAARRGYTPTVFFYRHNGEPGFFAADAMRDSLARALVAFYPVAGRLGLDGDGRVQVDCTGEGVVFATARSGHYALDDLMGEFVPCDEMRDLFVPAAPAAASCCPRGGALLLVQVTYLRCGGVVLGMALHHSIADGRSAAHFVETWASIARGAPAADAPVPPCFDHRLLAARPARAVLYDHPEYKPEPAPPARAATASTYASAIITLTKQQVGALRAACAGASTFRAVVALVWQCACRARALPPEAETRLHSMIDTRQRLSPPLPPGYFGNAVIRTSTAATAGEVVSSPVGHAARRARAATSQGEDYARSVVDYLEGVDAMNLPRSGVSRADLRAISWLGMSLADADFGWGSPAFMGPAIMYYSGFVYVMNAPGKDGAVALALSLEPESMPEFRKVFADEVARLA |
Proteomic databases
Expression
Tissue specificity
Expressed in roots.
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length433
- Mass (Da)45,686
- Last updated2004-12-21 v1
- ChecksumF9FD91827EDBEF96
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP003489 EMBL· GenBank· DDBJ | BAD72437.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP005919 EMBL· GenBank· DDBJ | BAD72530.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP008212 EMBL· GenBank· DDBJ | BAF18917.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP014962 EMBL· GenBank· DDBJ | BAS96510.1 EMBL· GenBank· DDBJ | Genomic DNA |