Q5RIU9 · SR5A3_DANRE
- ProteinPolyprenal reductase
- Genesrd5a3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids309 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Plays a key role in early steps of protein N-linked glycosylation by being involved in the conversion of polyprenol into dolichol (By similarity).
Acts as a polyprenal reductase that mediates the reduction of polyprenal into dolichal in a NADP-dependent mechanism (By similarity).
Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation (By similarity).
Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT) (By similarity).
Acts as a polyprenal reductase that mediates the reduction of polyprenal into dolichal in a NADP-dependent mechanism (By similarity).
Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation (By similarity).
Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT) (By similarity).
Catalytic activity
- a di-trans,poly-cis-dolichal + NADP+ = a di-trans,poly-cis-polyprenal + NADPH + H+This reaction proceeds in the backward direction.
a di-trans,poly-cis-dolichal RHEA-COMP:19537 + CHEBI:58349 = a di-trans,poly-cis-polyprenal RHEA-COMP:19536 + CHEBI:57783 + CHEBI:15378 - androst-4-ene-3,17-dione + NADPH + H+ = 5alpha-androstan-3,17-dione + NADP+This reaction proceeds in the backward direction.
Pathway
Protein modification; protein glycosylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | 3-oxo-5-alpha-steroid 4-dehydrogenase activity | |
Molecular Function | 3-oxo-5alpha-steroid 4-dehydrogenase (NADP+) activity | |
Molecular Function | oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor | |
Molecular Function | polyprenol reductase activity | |
Biological Process | dolichol biosynthetic process | |
Biological Process | dolichol metabolic process | |
Biological Process | dolichol-linked oligosaccharide biosynthetic process | |
Biological Process | polyprenol catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePolyprenal reductase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio
Accessions
- Primary accessionQ5RIU9
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Topological domain | 1-3 | Cytoplasmic | |||
Transmembrane | 4-24 | Helical | |||
Topological domain | 25-67 | Lumenal | |||
Transmembrane | 68-88 | Helical | |||
Topological domain | 89-114 | Cytoplasmic | |||
Transmembrane | 115-135 | Helical | |||
Topological domain | 136-150 | Lumenal | |||
Transmembrane | 151-171 | Helical | |||
Topological domain | 172-185 | Cytoplasmic | |||
Transmembrane | 186-206 | Helical | |||
Topological domain | 207-255 | Lumenal | |||
Transmembrane | 256-276 | Helical | |||
Topological domain | 277-309 | Cytoplasmic | |||
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000398651 | 1-309 | Polyprenal reductase | ||
Proteomic databases
Expression
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length309
- Mass (Da)35,708
- Last updated2004-12-21 v1
- Checksum89513C21F5D7FEB6
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 69 | in Ref. 2; AAI33965 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX088688 EMBL· GenBank· DDBJ | CAI11971.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC133964 EMBL· GenBank· DDBJ | AAI33965.1 EMBL· GenBank· DDBJ | mRNA |