Q5RE13 · RAB1B_PONAB

Function

function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity).
Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum (By similarity).
Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Required to modulate the compacted morphology of the Golgi. Promotes the recruitment of lipid phosphatase MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment (By similarity).

Miscellaneous

Rab-1B binds GTP and GDP and possesses intrinsic GTPase activity.

Catalytic activity

Activity regulation

Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP).

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site15-23GTP (UniProtKB | ChEBI)
Binding site33-40GTP (UniProtKB | ChEBI)
Binding site63-67GTP (UniProtKB | ChEBI)
Binding site121-124GTP (UniProtKB | ChEBI)
Binding site151-153GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentperinuclear region of cytoplasm
Cellular Componentphagophore assembly site membrane
Molecular FunctionG protein activity
Molecular FunctionGTP binding
Biological Processautophagy
Biological ProcessGolgi organization
Biological Processprotein transport
Biological Processvesicle-mediated transport

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ras-related protein Rab-1B
  • EC number

Gene names

    • Name
      RAB1B

Organism names

Accessions

  • Primary accession
    Q5RE13

Proteomes

Subcellular Location

Cytoplasm
Membrane
; Lipid-anchor
Preautophagosomal structure membrane
; Lipid-anchor
Note: Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm (By similarity).
Co-localizes with MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment and to the peri-Golgi region (By similarity).

Keywords

PTM/Processing

Features

Showing features for modified residue, chain, lipidation.

TypeIDPosition(s)Description
Modified residue1N-acetylmethionine
ChainPRO_00002605221-201
Lipidation200S-geranylgeranyl cysteine
Modified residue201Cysteine methyl ester
Lipidation201S-geranylgeranyl cysteine

Post-translational modification

Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1).

Keywords

Interaction

Subunit

Interacts with MICAL1 and MICAL2. Interacts (in GTP-bound form) with MICALCL, MICAL1 and MILCAL3. Interacts with GDI1; the interaction requires the GDP-bound state. Interacts with CHM/REP1; the interaction requires the GDP-bound form and is necessary for prenylation by GGTase II. Interacts with RabGAP TBC1D20. Interacts (in GDP-bound form) with lipid phosphatase MTMR6 (via GRAM domain); the interaction regulates MTMR6 recruitment to the endoplasmic reticulum-Golgi intermediate compartment (By similarity).
Interacts (in GDP-bound form) with lipid phosphatase MTMR7 (By similarity).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for motif, region.

TypeIDPosition(s)Description
Motif37-45Effector region
Region64-83Switch 2 region; required for interaction with REP1/CHM
Region174-201Disordered

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    201
  • Mass (Da)
    22,171
  • Last updated
    2004-12-21 v1
  • Checksum
    9812FF4DAC34B2BE
MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVGNKSDLTTKKVVDNTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASGGERPNLKIDSTPVKPAGGGCC

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
H2NCS1H2NCS1_PONAB199
A0A8I5TKD7A0A8I5TKD7_PONABRAB1B224
A0A2J8TZX3A0A2J8TZX3_PONABRAB1B169

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CR857726
EMBL· GenBank· DDBJ
CAH89994.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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