Q5RE13 · RAB1B_PONAB
- ProteinRas-related protein Rab-1B
- GeneRAB1B
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids201 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity).
Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum (By similarity).
Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Required to modulate the compacted morphology of the Golgi. Promotes the recruitment of lipid phosphatase MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment (By similarity).
Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum (By similarity).
Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Required to modulate the compacted morphology of the Golgi. Promotes the recruitment of lipid phosphatase MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment (By similarity).
Miscellaneous
Rab-1B binds GTP and GDP and possesses intrinsic GTPase activity.
Catalytic activity
- GTP + H2O = GDP + H+ + phosphateThis reaction proceeds in the forward direction.
Activity regulation
Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 15-23 | GTP (UniProtKB | ChEBI) | ||||
Sequence: GDSGVGKSC | ||||||
Binding site | 33-40 | GTP (UniProtKB | ChEBI) | ||||
Sequence: YTESYIST | ||||||
Binding site | 63-67 | GTP (UniProtKB | ChEBI) | ||||
Sequence: DTAGQ | ||||||
Binding site | 121-124 | GTP (UniProtKB | ChEBI) | ||||
Sequence: NKSD | ||||||
Binding site | 151-153 | GTP (UniProtKB | ChEBI) | ||||
Sequence: SAK |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | perinuclear region of cytoplasm | |
Cellular Component | phagophore assembly site membrane | |
Molecular Function | G protein activity | |
Molecular Function | GTP binding | |
Biological Process | autophagy | |
Biological Process | Golgi organization | |
Biological Process | protein transport | |
Biological Process | vesicle-mediated transport |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRas-related protein Rab-1B
- EC number
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Pongo
Accessions
- Primary accessionQ5RE13
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Lipid-anchor
Preautophagosomal structure membrane ; Lipid-anchor
Note: Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm (By similarity).
Co-localizes with MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment and to the peri-Golgi region (By similarity).
Co-localizes with MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment and to the peri-Golgi region (By similarity).
Keywords
- Cellular component
PTM/Processing
Features
Showing features for modified residue, chain, lipidation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 1 | N-acetylmethionine | ||||
Sequence: M | ||||||
Chain | PRO_0000260522 | 1-201 | Ras-related protein Rab-1B | |||
Sequence: MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVGNKSDLTTKKVVDNTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASGGERPNLKIDSTPVKPAGGGCC | ||||||
Lipidation | 200 | S-geranylgeranyl cysteine | ||||
Sequence: C | ||||||
Modified residue | 201 | Cysteine methyl ester | ||||
Sequence: C | ||||||
Lipidation | 201 | S-geranylgeranyl cysteine | ||||
Sequence: C |
Post-translational modification
Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1).
Keywords
- PTM
Interaction
Subunit
Interacts with MICAL1 and MICAL2. Interacts (in GTP-bound form) with MICALCL, MICAL1 and MILCAL3. Interacts with GDI1; the interaction requires the GDP-bound state. Interacts with CHM/REP1; the interaction requires the GDP-bound form and is necessary for prenylation by GGTase II. Interacts with RabGAP TBC1D20. Interacts (in GDP-bound form) with lipid phosphatase MTMR6 (via GRAM domain); the interaction regulates MTMR6 recruitment to the endoplasmic reticulum-Golgi intermediate compartment (By similarity).
Interacts (in GDP-bound form) with lipid phosphatase MTMR7 (By similarity).
Interacts (in GDP-bound form) with lipid phosphatase MTMR7 (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for motif, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 37-45 | Effector region | ||||
Sequence: YISTIGVDF | ||||||
Region | 64-83 | Switch 2 region; required for interaction with REP1/CHM | ||||
Sequence: TAGQERFRTITSSYYRGAHG | ||||||
Region | 174-201 | Disordered | ||||
Sequence: GPGAASGGERPNLKIDSTPVKPAGGGCC |
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length201
- Mass (Da)22,171
- Last updated2004-12-21 v1
- Checksum9812FF4DAC34B2BE
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
H2NCS1 | H2NCS1_PONAB | 199 | |||
A0A8I5TKD7 | A0A8I5TKD7_PONAB | RAB1B | 224 | ||
A0A2J8TZX3 | A0A2J8TZX3_PONAB | RAB1B | 169 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CR857726 EMBL· GenBank· DDBJ | CAH89994.1 EMBL· GenBank· DDBJ | mRNA |