Q5R981 · CMTR1_PONAB
- ProteinCap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1
- GeneCMTR1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids835 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap1 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the first nucleotide of a m7GpppG-capped mRNA and small nuclear RNA (snRNA) to produce m7GpppRm (cap1). Displays a preference for cap0 transcripts. Cap1 modification is linked to higher levels of translation. May be involved in the interferon response pathway.
Catalytic activity
- a 5'-end (N7-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside) in mRNA + H+ + S-adenosyl-L-homocysteine
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 203-207 | substrate | ||||
Sequence: KSVFD | ||||||
Binding site | 218 | substrate | ||||
Sequence: R | ||||||
Binding site | 234 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Active site | 239 | |||||
Sequence: K | ||||||
Binding site | 277-283 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: CAGPGGF | ||||||
Binding site | 335-336 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: DI | ||||||
Active site | 364 | |||||
Sequence: D | ||||||
Binding site | 374-376 | substrate | ||||
Sequence: NLQ | ||||||
Active site | 404 | Proton acceptor | ||||
Sequence: K | ||||||
Binding site | 439 | substrate | ||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleoplasm | |
Cellular Component | nucleus | |
Molecular Function | mRNA (nucleoside-2'-O-)-methyltransferase activity | |
Molecular Function | nucleic acid binding | |
Biological Process | 7-methylguanosine mRNA capping | |
Biological Process | methylation | |
Biological Process | mRNA processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Pongo
Accessions
- Primary accessionQ5R981
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000251241 | 1-835 | Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 | |||
Sequence: MKRRTDPECTAPIKKQKKRVAELALSLSSTSDDEPPSSVSHGAKASTTSLSGSDSETEGKQHSSDSFDDAFKADSLVEGTSSRYSMYNSVSQKLMAKMGFREGEGLGKYSQGRKDIVEASSQKGRRGLGLTLRGFDQELNVDWRDEPEPSACEQVSWFPECTTEIPDTQEMSDWMVVGKRKMIIEDETEFCGEELLHSVLQCKSVFDVLDGEEMRRARTRANPYEMIRGVFFLNRAAMKMANMDFVFDRMFTNPRDSYGKPLVKDREAELLYFADVCAGPGGFSEYVLWRKKWHAKGFGMTLKGPNDFKLEDFYSASSELFEPYYGEGGIDGDGDITRPENISAFRNFVLDNTDRKGVHFLMADGGFSVEGQENLQEILSKQLLLCQFLMALSIVRTGGHFICKTFDLFTPFSVGLVYLLYCCFERVCLFKPITSRPANSERYVVCKGLKVGIDDVRDYLFAVNIKLNQLRNTDSDVNLVVPLEVIKGDHEFTDYMIRSNESHCSLQIKALAKIHAFVQDTTLSEPRQAEIRKECLRLWGIPDQARVAPSSSDPKSKFFELIQGTEIDIFSYKPTLLTSKTLEKIRPVFDYRCMVSGSEQKFLIGLGKSQIYTWDGRQSDRWIKLDLKTELPRDTLLSVEIVHELKGEGKAQRKISAIHILDVLVLNGTDVREQHFNQRIQLAEKFVKAVSKPSRPDMNPIRVKEVYRLEEMEKIFVRLEMKIIKGSSGTPKLSYTGRDDRHFVPMGLYIVRTVNEPWTMGFSKSFKKKFFYNKKTKDSTFDLPADSIAPFHICYYGRLFWEWGDGIRVHDSQKPQDQDKLSKEDVLSFIQMHRA | ||||||
Modified residue | 28 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 31 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 53 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 66 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 91 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 108 | N6-acetyllysine | ||||
Sequence: K |
Keywords
- PTM
Interaction
Structure
Family & Domains
Features
Showing features for compositional bias, region, motif, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-21 | Basic and acidic residues | ||||
Sequence: MKRRTDPECTAPIKKQKKRVA | ||||||
Region | 1-67 | Disordered | ||||
Sequence: MKRRTDPECTAPIKKQKKRVAELALSLSSTSDDEPPSSVSHGAKASTTSLSGSDSETEGKQHSSDSF | ||||||
Motif | 2-19 | Bipartite nuclear localization signal | ||||
Sequence: KRRTDPECTAPIKKQKKR | ||||||
Compositional bias | 23-57 | Polar residues | ||||
Sequence: LALSLSSTSDDEPPSSVSHGAKASTTSLSGSDSET | ||||||
Domain | 87-133 | G-patch | ||||
Sequence: YNSVSQKLMAKMGFREGEGLGKYSQGRKDIVEASSQKGRRGLGLTLR | ||||||
Domain | 231-450 | RrmJ-type SAM-dependent 2'-O-MTase | ||||
Sequence: FFLNRAAMKMANMDFVFDRMFTNPRDSYGKPLVKDREAELLYFADVCAGPGGFSEYVLWRKKWHAKGFGMTLKGPNDFKLEDFYSASSELFEPYYGEGGIDGDGDITRPENISAFRNFVLDNTDRKGVHFLMADGGFSVEGQENLQEILSKQLLLCQFLMALSIVRTGGHFICKTFDLFTPFSVGLVYLLYCCFERVCLFKPITSRPANSERYVVCKGLK | ||||||
Region | 727-835 | Interaction with POLR2A | ||||
Sequence: SSGTPKLSYTGRDDRHFVPMGLYIVRTVNEPWTMGFSKSFKKKFFYNKKTKDSTFDLPADSIAPFHICYYGRLFWEWGDGIRVHDSQKPQDQDKLSKEDVLSFIQMHRA | ||||||
Domain | 752-786 | WW | ||||
Sequence: RTVNEPWTMGFSKSFKKKFFYNKKTKDSTFDLPAD |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length835
- Mass (Da)95,321
- Last updated2004-12-21 v1
- Checksum03919512A73C30FB
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
H2PIW9 | H2PIW9_PONAB | CMTR1 | 804 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-21 | Basic and acidic residues | ||||
Sequence: MKRRTDPECTAPIKKQKKRVA | ||||||
Compositional bias | 23-57 | Polar residues | ||||
Sequence: LALSLSSTSDDEPPSSVSHGAKASTTSLSGSDSET |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CR859510 EMBL· GenBank· DDBJ | CAH91679.1 EMBL· GenBank· DDBJ | mRNA |