Q5R8M1 · STK38_PONAB

Function

function

Serine/threonine-protein kinase that acts as a negative regulator of MAP3K1/2 signaling. Converts MAP3K2 from its phosphorylated form to its non-phosphorylated form and inhibits autophosphorylation of MAP3K2. Acts as an ufmylation 'reader' in a kinase-independent manner: specifically recognizes and binds mono-ufmylated histone H4 in response to DNA damage, promoting the recruitment of SUV39H1 to the double-strand breaks, resulting in ATM activation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Activated by binding of S100B which releases autoinhibitory N-lobe interactions, enabling ATP to bind and the autophosphorylation of Ser-281. Thr-444 then undergoes calcium-dependent phosphorylation by STK24/MST3. Interactions between phosphorylated Thr-444 and the N-lobe promote additional structural changes that complete the activation of the kinase. Autoinhibition is also released by the binding of MOB1/MOBKL1A and MOB2/HCCA2 to the N-terminal of STK38 (By similarity).

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site95-103ATP (UniProtKB | ChEBI)
Binding site118ATP (UniProtKB | ChEBI)
Active site212Proton acceptor

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentnucleus
Cellular Componentsite of double-strand break
Molecular FunctionATP binding
Molecular Functionhistone reader activity
Molecular Functionmagnesium ion binding
Molecular Functionmitogen-activated protein kinase kinase kinase binding
Molecular Functionprotein serine kinase activity
Molecular Functionprotein serine/threonine kinase activity
Molecular FunctionUFM1-modified protein reader activity
Biological ProcessDNA damage checkpoint signaling
Biological ProcessDNA damage response
Biological Processnegative regulation of MAP kinase activity
Biological Processprotein modification process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Serine/threonine-protein kinase 38
  • EC number

Gene names

    • Name
      STK38

Organism names

Accessions

  • Primary accession
    Q5R8M1

Proteomes

Subcellular Location

Nucleus
Cytoplasm
Chromosome
Note: Localizes to DNA double-strand breaks in response to DNA damage.

Keywords

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylalanine
ChainPRO_00002929532-465Serine/threonine-protein kinase 38
Modified residue74Phosphothreonine
Modified residue264Phosphoserine
Modified residue281Phosphoserine; by autocatalysis
Modified residue444Phosphothreonine; by STK24/MST3

Post-translational modification

ISGylated.
Phosphorylated by STK3/MST2 and this is enhanced by MOBKL1B.

Keywords

Interaction

Subunit

Homodimeric S100B binds two molecules of STK38. Interacts with MOB1 and MOB2. Interacts with MAP3K1 and MAP3K2 (via the kinase domain). Forms a tripartite complex with MOBKL1B and STK3/MST2. Interacts with MICAL1; leading to inhibit the protein kinase activity by antagonizing activation by MST1/STK4.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain, motif.

TypeIDPosition(s)Description
Region62-87Interaction with S100B
Domain89-382Protein kinase
Motif306-311UFM1-interacting motif (UFIM)
Domain383-455AGC-kinase C-terminal

Domain

The UFM1-interacting motif (UFIM) specifically recognizes and binds ufmylated histone H4.

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    465
  • Mass (Da)
    54,190
  • Last updated
    2004-12-21 v1
  • Checksum
    7262221DBFFAF83C
MAMTGSTPCSSMSNHTKERVTMTKVTLENFYSNLIAQHEEREMRQKKLEKVMEEEGLKDEEKRLRRSAHARKETEFLRLKRTRLGLEDFESLKVIGRGAFGEVRLVQKKDTGHVYAMKILRKADMLEKEQVGHIRAERDILVEADSLWVVKMFYSFQDKLNLYLIMEFLPGGDMMTLLMKKDTLTEEETQFYIAETVLAIDSIHQLGFIHRDIKPDNLLLDSKGHVKLSDFGLCTGLKKAHRTEFYRNLNHSLPSDFTFQNMNSKRKAETWKRNRRQLAFSTVGTPDYIAPEVFMQTGYNKLCDWWSLGVIMYEMLIGYPPFCSETPQETYKKVMNWKETLTFPPEVPISEKAKDLILRFCCEWEHRIGAPGVEEIKSNSFFEGVDWEHIRERPAAISIEIKSIDDTSNFDEFPESDILKPTVATSNHPETDYKNKDWVFINYTYKRFEGLTARGAIPSYMKAAK

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8I5TIU1A0A8I5TIU1_PONABSTK38450

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CR859730
EMBL· GenBank· DDBJ
CAH91889.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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