Q5R7J6 · UBE2N_PONAB
- ProteinUbiquitin-conjugating enzyme E2 N
- GeneUBE2N
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids152 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production (By similarity).
UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway
UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway
Catalytic activity
Activity regulation
Activity is inhibited by binding to OTUB1, which prevents 'Lys-63'-linked polyubiquitination (By similarity).
Activity is inhibited by GPS2, leading to prevent 'Lys-63'-linked polyubiquitination (By similarity).
Activity is inhibited by GPS2, leading to prevent 'Lys-63'-linked polyubiquitination (By similarity).
Pathway
Protein modification; protein ubiquitination.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 87 | Glycyl thioester intermediate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Cellular Component | UBC13-MMS2 complex | |
Cellular Component | ubiquitin ligase complex | |
Molecular Function | ATP binding | |
Molecular Function | ubiquitin conjugating enzyme activity | |
Molecular Function | ubiquitin-protein transferase activity | |
Biological Process | postreplication repair | |
Biological Process | protein K63-linked ubiquitination |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUbiquitin-conjugating enzyme E2 N
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Pongo
Accessions
- Primary accessionQ5R7J6
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain, modified residue, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000082505 | 1-152 | Ubiquitin-conjugating enzyme E2 N | |||
Sequence: MAGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAMNNI | ||||||
Modified residue | 82 | N6-acetyllysine | ||||
Sequence: K | ||||||
Cross-link | 92 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15) | ||||
Sequence: K |
Post-translational modification
Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
Keywords
- PTM
Interaction
Subunit
Heterodimer with UBE2V2 (By similarity).
Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific 'Lys-63'-linked polyubiquitination activity (By similarity).
Interacts with RNF8 and RNF168 (By similarity).
Interacts with RNF11 (By similarity).
Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the 'Lys-63'-linked polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair (By similarity).
Interacts with ARIH2 (via RING-type 2) (By similarity).
Interacts with OTUB1; leading to inhibit E2-conjugating activity (By similarity).
Interacts with GPS2; leading to inhibit E2-conjugating activity (By similarity).
Interacts with RIGI and RNF135; involved in RIGI ubiquitination and activation (By similarity).
Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific 'Lys-63'-linked polyubiquitination activity (By similarity).
Interacts with RNF8 and RNF168 (By similarity).
Interacts with RNF11 (By similarity).
Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the 'Lys-63'-linked polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair (By similarity).
Interacts with ARIH2 (via RING-type 2) (By similarity).
Interacts with OTUB1; leading to inhibit E2-conjugating activity (By similarity).
Interacts with GPS2; leading to inhibit E2-conjugating activity (By similarity).
Interacts with RIGI and RNF135; involved in RIGI ubiquitination and activation (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 3-149 | UBC core | ||||
Sequence: GLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAM |
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length152
- Mass (Da)17,138
- Last updated2004-12-21 v1
- ChecksumFACD84D883D77407
Computationally mapped potential isoform sequences
There are 5 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8I5U8A0 | A0A8I5U8A0_PONAB | UBE2N | 149 | ||
A0A2J8XN72 | A0A2J8XN72_PONAB | UBE2N | 105 | ||
A0A8I5YLG9 | A0A8I5YLG9_PONAB | UBE2N | 200 | ||
A0A8I5YST4 | A0A8I5YST4_PONAB | UBE2N | 193 | ||
A0A8I5YSZ1 | A0A8I5YSZ1_PONAB | UBE2N | 168 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CR860119 EMBL· GenBank· DDBJ | CAH92264.1 EMBL· GenBank· DDBJ | mRNA |