Q5PPH0 · ENOPH_RAT
- ProteinEnolase-phosphatase E1
- GeneEnoph1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids261 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).
Catalytic activity
- 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | 2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity | |
Molecular Function | 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity | |
Molecular Function | acireductone synthase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | L-methionine salvage from methylthioadenosine | |
Biological Process | L-methionine salvage from S-adenosylmethionine |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEnolase-phosphatase E1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ5PPH0
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000254009 | 1-261 | Enolase-phosphatase E1 | |||
Sequence: MVVVSVPAEVTVILLDIEGTTTPIAFVKDILFPYIKENVKEYLQTHWEEEECQQDVSLLRKQAEEDAHLDGAVPIPVASGGDVQQMIQAVVDNVSWQMSHDRKTTALKQLQGHMWKAAFTAGRMKAEVFADVVPAVRRWREAGMKVYIYSSGSVEAQKLLFGHSTEGDILELIDGHFDTKIGHKVESDSYRKIADSIGCSTNNILFLTDVTVEASAAEEADVHVAVVVRPGNAGLTDDEKTYYNLISSFSELYLPSTQSKV |
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length261
- Mass (Da)28,875
- Last updated2005-01-04 v1
- Checksum0C51CA857A122BEE
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8I6ADH3 | A0A8I6ADH3_RAT | Enoph1 | 274 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC087697 EMBL· GenBank· DDBJ | AAH87697.1 EMBL· GenBank· DDBJ | mRNA |