Q5PG96 · HPCH_SALPA
- Protein4-hydroxy-2-oxo-heptane-1,7-dioate aldolase
- GenehpcH
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids263 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reversible retro-aldol cleavage of 4-hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde.
Catalytic activity
- 4-hydroxy-2-oxoheptanedioate = pyruvate + succinate semialdehyde
Cofactor
Note: Binds 1 divalent metal cation per subunit.
Pathway
Aromatic compound metabolism; 4-hydroxyphenylacetate degradation; pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate: step 7/7.
Features
Showing features for active site, site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 45 | Proton acceptor | ||||
Sequence: H | ||||||
Site | 70 | Transition state stabilizer | ||||
Sequence: R | ||||||
Site | 84 | Increases basicity of active site His | ||||
Sequence: D | ||||||
Binding site | 147 | substrate | ||||
Sequence: Q | ||||||
Binding site | 149 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 174 | substrate | ||||
Sequence: A | ||||||
Binding site | 175 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 175 | substrate | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity | |
Molecular Function | 4-hydroxy-2-ketopimelate aldolase activity | |
Molecular Function | metal ion binding | |
Biological Process | phenylacetate catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name4-hydroxy-2-oxo-heptane-1,7-dioate aldolase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella
Accessions
- Primary accessionQ5PG96
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000355108 | 1-263 | 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase | |||
Sequence: MKNAFKDALKAGRPQIGLWLGLANSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGSALARASRWNRIPDYLHLANDAMCVLVQIETREAMSNLASILDVDGIDGVFIGPADLSADMGFAGNPQHPEVQAAIENAIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVDTTLLARGAEALAARFGAEKNLSGASGVY |
Interaction
Subunit
Homohexamer; trimer of dimers.
Structure
Sequence
- Sequence statusComplete
- Length263
- Mass (Da)27,758
- Last updated2005-01-04 v1
- Checksum52CC25BC0244BBB9
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000026 EMBL· GenBank· DDBJ | AAV77664.1 EMBL· GenBank· DDBJ | Genomic DNA |