Q5PC82 · CCA_SALPA

Function

function

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded.

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Magnesium is required for nucleotidyltransferase activity.
Ni2+ (UniProtKB | Rhea| CHEBI:49786 )

Note: Nickel for phosphatase activity.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site8ATP (UniProtKB | ChEBI)
Binding site8CTP (UniProtKB | ChEBI)
Binding site11ATP (UniProtKB | ChEBI)
Binding site11CTP (UniProtKB | ChEBI)
Binding site21Mg2+ (UniProtKB | ChEBI)
Binding site23Mg2+ (UniProtKB | ChEBI)
Binding site91ATP (UniProtKB | ChEBI)
Binding site91CTP (UniProtKB | ChEBI)
Binding site137ATP (UniProtKB | ChEBI)
Binding site137CTP (UniProtKB | ChEBI)
Binding site140ATP (UniProtKB | ChEBI)
Binding site140CTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular Functioncyclic-nucleotide phosphodiesterase activity
Molecular Functionmagnesium ion binding
Molecular Functionphosphatase activity
Molecular FunctiontRNA binding
Biological ProcessRNA repair
Biological ProcesstRNA 3'-terminal CCA addition

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Multifunctional CCA protein

Including 4 domains:

  • Recommended name
    CCA-adding enzyme
  • EC number
  • Alternative names
    • CCA tRNA nucleotidyltransferase
    • tRNA CCA-pyrophosphorylase
    • tRNA adenylyl-/cytidylyl-transferase
    • tRNA nucleotidyltransferase
    • tRNA-NT
  • Recommended name
    2'-nucleotidase
  • EC number
  • Recommended name
    2',3'-cyclic phosphodiesterase
  • EC number
  • Recommended name
    Phosphatase
  • EC number

Gene names

    • Name
      cca
    • Ordered locus names
      SPA3072

Organism names

Accessions

  • Primary accession
    Q5PC82

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001389981-413Multifunctional CCA protein

Interaction

Subunit

Monomer. Can also form homodimers and oligomers.

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain228-329HD

Domain

Comprises two domains: an N-terminal domain containing the nucleotidyltransferase activity and a C-terminal HD domain associated with both phosphodiesterase and phosphatase activities.

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    413
  • Mass (Da)
    46,606
  • Last updated
    2005-01-04 v1
  • Checksum
    D9184864C17D81AE
MKIYLVGGAVRDALLGLPVKDKDWVVVGATPQEMLDAGYQQVGRDFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEADLQRRDLTINALARDDDGQIIDPYHGRRDLEARLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALMREMTAAGELEHLTPERVWKETENALTTRNPQVYFQVLRDCGALRVLFPEIDALFGVPAPAKWHPEIDTGVHTLMTLSMAAMLSPQLDVRFATLCHDLGKGLTPKNLWPRHHGHGPAGVKLVEQLCQRLRVPNDLRDLAKLVAEYHDLIHTFPILQPKTIVKLFDAIDAWRKPQRVEQIALTSEADVRGRTGFEASDYPQGRWLREAWQVAQAVPTKEVVEAGFKGIEIREELTKRRIAAVANWKEKRCPNPAS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000026
EMBL· GenBank· DDBJ
AAV78907.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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