Q5NUF3 · HIDH_SOYBN

Function

function

Dehydratase that mediates the biosynthesis of isoflavonoids. Can use both 4'-hydroxylated and 4'-methoxylated 2-hydroxyisoflavanones as substrates. Has also a slight carboxylesterase activity toward p-nitrophenyl butyrate.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
29 μM2,7-dihydroxy-4'-methoxyisoflavanone7.530
114 μM2,7,4'-trihydroxyisoflavanone7.530
170 μM2,5,7,4'-tetrahydroxyisoflavanone7.530
kcat is 1.6 sec-1 with 2,7-dihydroxy-4'-methoxyisoflavanone, 5.3 sec-1 with 2,7,4'-trihydroxyisoflavanone and 18.1 sec-1 with 2,5,7,4'-tetrahydroxyisoflavanone as substrates, respectively (at pH 7.5 and 30 degrees Celsius).

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site164
Active site263
Active site295

GO annotations

AspectTerm
Molecular Function2-hydroxyisoflavanone dehydratase activity
Molecular Functioncarboxylesterase activity
Molecular Functioncarboxylic ester hydrolase activity
Biological Processflavonoid biosynthetic process
Biological Processisoflavonoid biosynthetic process
Biological Processisoflavonoid metabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

Gene names

    • Name
      HIDH
    • Ordered locus names
      Glyma01g45020

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Williams 82
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > indigoferoid/millettioid clade > Phaseoleae > Glycine > Glycine subgen. Soja

Accessions

  • Primary accession
    Q5NUF3

Proteomes

Genome annotation databases

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis78Reduction of both dehydratase and carboxylesterase activities.
Mutagenesis79Reduction of both dehydratase and carboxylesterase activities.
Mutagenesis164Reduction of both dehydratase and carboxylesterase activities.
Mutagenesis263Complete loss of both dehydratase and carboxylesterase activities.
Mutagenesis295Complete loss of both dehydratase and carboxylesterase activities.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004241011-3192-hydroxyisoflavanone dehydratase

Proteomic databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif77-79Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole

Sequence similarities

Belongs to the 'GDXG' lipolytic enzyme family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    319
  • Mass (Da)
    35,138
  • Last updated
    2005-02-01 v1
  • Checksum
    E8333CF425FBA4A3
MAKEIVKELLPLIRVYKDGSVERLLSSENVAASPEDPQTGVSSKDIVIADNPYVSARIFLPKSHHTNNKLPIFLYFHGGAFCVESAFSFFVHRYLNILASEANIIAISVDFRLLPHHPIPAAYEDGWTTLKWIASHANNTNTTNPEPWLLNHADFTKVYVGGETSGANIAHNLLLRAGNESLPGDLKILGGLLCCPFFWGSKPIGSEAVEGHEQSLAMKVWNFACPDAPGGIDNPWINPCVPGAPSLATLACSKLLVTITGKDEFRDRDILYHHTVEQSGWQGELQLFDAGDEEHAFQLFKPETHLAKAMIKRLASFLV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB154415
EMBL· GenBank· DDBJ
BAD80840.1
EMBL· GenBank· DDBJ
mRNA
BT097440
EMBL· GenBank· DDBJ
ACU22699.1
EMBL· GenBank· DDBJ
mRNA
CM000834
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

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