Q5NUF3 · HIDH_SOYBN
- Protein2-hydroxyisoflavanone dehydratase
- GeneHIDH
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids319 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Dehydratase that mediates the biosynthesis of isoflavonoids. Can use both 4'-hydroxylated and 4'-methoxylated 2-hydroxyisoflavanones as substrates. Has also a slight carboxylesterase activity toward p-nitrophenyl butyrate.
Catalytic activity
- (2R,3S)-2,4',7-trihydroxyisoflavanone = daidzein + H+ + H2O
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
29 μM | 2,7-dihydroxy-4'-methoxyisoflavanone | 7.5 | 30 | |||
114 μM | 2,7,4'-trihydroxyisoflavanone | 7.5 | 30 | |||
170 μM | 2,5,7,4'-tetrahydroxyisoflavanone | 7.5 | 30 |
kcat is 1.6 sec-1 with 2,7-dihydroxy-4'-methoxyisoflavanone, 5.3 sec-1 with 2,7,4'-trihydroxyisoflavanone and 18.1 sec-1 with 2,5,7,4'-tetrahydroxyisoflavanone as substrates, respectively (at pH 7.5 and 30 degrees Celsius).
Pathway
Secondary metabolite biosynthesis; flavonoid biosynthesis.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 164 | |||||
Sequence: T | ||||||
Active site | 263 | |||||
Sequence: D | ||||||
Active site | 295 | |||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 2-hydroxyisoflavanone dehydratase activity | |
Molecular Function | carboxylesterase activity | |
Molecular Function | carboxylic ester hydrolase activity | |
Biological Process | flavonoid biosynthetic process | |
Biological Process | isoflavonoid biosynthetic process | |
Biological Process | isoflavonoid metabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended name2-hydroxyisoflavanone dehydratase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > indigoferoid/millettioid clade > Phaseoleae > Glycine > Glycine subgen. Soja
Accessions
- Primary accessionQ5NUF3
Proteomes
Genome annotation databases
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 78 | Reduction of both dehydratase and carboxylesterase activities. | ||||
Sequence: G → A | ||||||
Mutagenesis | 79 | Reduction of both dehydratase and carboxylesterase activities. | ||||
Sequence: G → A | ||||||
Mutagenesis | 164 | Reduction of both dehydratase and carboxylesterase activities. | ||||
Sequence: T → A or S | ||||||
Mutagenesis | 263 | Complete loss of both dehydratase and carboxylesterase activities. | ||||
Sequence: D → N | ||||||
Mutagenesis | 295 | Complete loss of both dehydratase and carboxylesterase activities. | ||||
Sequence: H → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000424101 | 1-319 | 2-hydroxyisoflavanone dehydratase | |||
Sequence: MAKEIVKELLPLIRVYKDGSVERLLSSENVAASPEDPQTGVSSKDIVIADNPYVSARIFLPKSHHTNNKLPIFLYFHGGAFCVESAFSFFVHRYLNILASEANIIAISVDFRLLPHHPIPAAYEDGWTTLKWIASHANNTNTTNPEPWLLNHADFTKVYVGGETSGANIAHNLLLRAGNESLPGDLKILGGLLCCPFFWGSKPIGSEAVEGHEQSLAMKVWNFACPDAPGGIDNPWINPCVPGAPSLATLACSKLLVTITGKDEFRDRDILYHHTVEQSGWQGELQLFDAGDEEHAFQLFKPETHLAKAMIKRLASFLV |
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 77-79 | Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole | ||||
Sequence: HGG |
Sequence similarities
Belongs to the 'GDXG' lipolytic enzyme family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length319
- Mass (Da)35,138
- Last updated2005-02-01 v1
- ChecksumE8333CF425FBA4A3
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB154415 EMBL· GenBank· DDBJ | BAD80840.1 EMBL· GenBank· DDBJ | mRNA | ||
BT097440 EMBL· GenBank· DDBJ | ACU22699.1 EMBL· GenBank· DDBJ | mRNA | ||
CM000834 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |