Q5ND34 · WDR81_MOUSE
- ProteinWD repeat-containing protein 81
- GeneWdr81
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1934 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Functions as a negative regulator of the PI3 kinase/PI3K activity associated with endosomal membranes via BECN1, a core subunit of the PI3K complex. By modifying the phosphatidylinositol 3-phosphate/PtdInsP3 content of endosomal membranes may regulate endosome fusion, recycling, sorting and early to late endosome transport. It is for instance, required for the delivery of cargos like BST2/tetherin from early to late endosome and thereby participates indirectly to their degradation by the lysosome. May also play a role in aggrephagy, the macroautophagic degradation of ubiquitinated protein aggregates. In this process, may regulate the interaction of SQSTM1 with ubiquitinated proteins and also recruit MAP1LC3C (By similarity).
May also be involved in maintenance of normal mitochondrial structure and organization (PubMed:23595742).
May also be involved in maintenance of normal mitochondrial structure and organization (PubMed:23595742).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | autophagosome membrane | |
Cellular Component | cytosol | |
Cellular Component | early endosome membrane | |
Cellular Component | endosome membrane | |
Cellular Component | late endosome membrane | |
Cellular Component | lysosomal membrane | |
Cellular Component | mitochondrion | |
Molecular Function | K63-linked polyubiquitin modification-dependent protein binding | |
Molecular Function | phosphatidylinositol 3-kinase inhibitor activity | |
Molecular Function | phosphatidylinositol 3-kinase regulator activity | |
Biological Process | aggrephagy | |
Biological Process | early endosome to late endosome transport | |
Biological Process | mitochondrion organization | |
Biological Process | protein stabilization | |
Biological Process | ubiquitin-dependent protein catabolic process |
Names & Taxonomy
Protein names
- Recommended nameWD repeat-containing protein 81
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ5ND34
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Early endosome membrane ; Peripheral membrane protein
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Brain-specific, conditional knockout mice are born at the expected Mendelian frequency, but most of them die a few hours after birth. If they show no overt brain morphological abnormalities, a neuronal accumulation of SQSTM1 in foci is observed.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 1349 | Progressive ataxia associated with Purkinje cell death. Retinal thinning due to photoreceptor cell death. | ||||
Sequence: L → P |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 62 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000247245 | 1-1934 | WD repeat-containing protein 81 | |||
Sequence: MAQGSRRRKVVLTAGSEGWSPSSGPDMEELLRSVERDLNIDARQLALAPGGTHVVALVSTRWLASLRERRLGPCPRAEGLGEAEVRTLLQRSVQRLPPGWTRVEVHGLRKRRLSYPLGGGVPFEEGSCSPETLTRFMQEVAAQNYRNLWRHAYHTYGQPYSHSTAPSALPALDSIRQALQRVYGCTFLPVGESIPCLSNVRDGPCPSRGSPACPSLLRAEALLESPEMLYVVHPYVQFSLHDVVTFSPAKLTNSQAKVLFLLFRVLRAMDACHRQGLACGALSLHHIAVDEKLCSELRLDLSAYEMPSEDENQEGSEEKNGTGIKSEKEGEGRTECPTCQKELRGLVLDWVHGRISNFHYLMQLNRLAGRRQGDPNYHPVLPWVVDFTTPYGRFRDLRKSKFRLNKGDKQLDFTYEMTRQAFVAGGAGSGEPPHVPHHISDVLSDITYYVYKARRTPRSVLCGHVRAQWEPHEYPATMERMQTWTPDECIPEFYTDPSIFCSIHPDMPDLDVPAWCSSNQEFVAAHRALLESWEVSQDLHHWIDLTFGYKLQGKEAVKEKNVCLHLVDAHTHLTSYGVVQLFDQPHPQRLAGSPALAPEPPLIPRLLVQPIREATGQEDISGQLINGAGRLVVEATPCETGWTRDRPGTGEDDLEQATEALDSISLPGKAGDQPGSSSSQASPGLLSFSAPSGSRPGRRSKAAGLDPGEGEEGKIVLPEGFSPIQALEELEKVGNFLAKGLGSQLEEPEKPHAQPPVHLQSLFHRDMQVLGVLLAEMVFATRVRILQPDAPLWVRFEAVRGLCIRHSKDIPVSLQPVLDTLLQLSGPKSPMVSKKGKLDPLFEYRPVSQGLPPPSPAQLLSPFSSVVPFPPYFPALHKFILLYQARRVEDEVQGRELAFALWQQLGAVLNDITPEGLEILLPFVLSLMSEEHTAVYTAWYLFEPVAKALGPKNANKYLLKPLIGAYESPCRLHGRFYLYTDCFVAQLVVRLGLQAFLTHLLPHVLQVLAGVEASQEEGKGLVGTTEDEESELPVSGPGSCAFGEEIQMDGQPAASSGLGLPDYRSGVSFHDQADLPDTEDFQAGLYVAESPQPQEAEAVSLGQLSDKSSTSEASQGEERGGDDGGAPADKNSVKSGDSSQDLKQSEGSEEEEEEEGCVVLEEDQEDEVTGTSELTLSDTMLSMETVVAPGDGRDREEEEEPLTEQTEGKEQKILLDTACKMVRWLSAKLGPTVASRHVARNLLRLLTSCYVGPTRQQFTVSSDDTPPLNAGNIYQKRPVLGDIVSGPVLSCLLHIAYLYGEPVLTYQYLPYISYLVAPGSNSNPSRLNSRKEAGLLAAVTLTQKIIVYLSDTTLMDILPRISHEVLLPVLGFLTSFVTGFPSGAQARTVLCVKTISLIALICLRIGQEMVQQHLSEPVATFFQVFSHLHELRQQDLPLDPKGCTEGQLPEATFSDGQRRPVDPTLLEELQKVFTLEMAYTIYVPFSCLLGDIIRKIIPNHELVGELAGLYLESMSPSSRNPASMEPTMASAGPEWDPQSGSCLQDDGHSGTFGSVLVGNRIQIPDSQPQSPGPLGSLSGVGSSGGLSNRNEDNALKRELPRSAHGLSGNWLAYWQYEIGVSQQDAHFHFHQIRLQSFPGHTGAVKCVAALSSEDFFLSGSKDRTVRLWPLYNYGDGTNETASRLIYAQHRKSVFYVGQLEAPQYVVSCDGAVHVWDPFTGKTLRTVDPSDSRVPLTAVAVMPAPHTSITMASSDSTLRFVDCRKPGLQHEFRLGGGLNPGLVRSLAVSPSGRSVVAGFSSGFMVLLDTRTGLVLRGWPAHEGDILQIKAVEGSVLISSSSDHSLTVWKELEQKPTHHYKSASDPIHTFDLYGSEVVTGTVANKIGVCSLLEPPSQATTKLSSENFRGTLTSLALLPTKRHLLLGSDNGIIRLLA |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in brain, Purkinje cells of cerebellum, retinal photoreceptor cells and spinal cord (at protein level) (PubMed:23595742).
In brain, specifically expressed by neuronal cells (at protein level). Detected in a wide range of tissues including intestine, adipose tissue, liver, pancreas, thymus, spleen, kidney, heart, eye, sciatic nerve, and testis (PubMed:21885617, PubMed:23595742, PubMed:28404643).
In brain, specifically expressed by neuronal cells (at protein level). Detected in a wide range of tissues including intestine, adipose tissue, liver, pancreas, thymus, spleen, kidney, heart, eye, sciatic nerve, and testis (PubMed:21885617, PubMed:23595742, PubMed:28404643).
Gene expression databases
Interaction
Subunit
Interacts with WDR91; involved in early to late endosome cargo transport. Interacts with BECN1; negatively regulates the PI3 kinase/PI3K activity associated with endosomal membranes. Interacts with SQSTM1; the interaction is direct and regulates the interaction of SQSTM1 with ubiquitinated proteins. Interacts with MAP1LC3C; recruits MAP1LC3C to ubiquitinated protein aggregates in the aggrephagy process.
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-26 | Disordered | ||||
Sequence: MAQGSRRRKVVLTAGSEGWSPSSGPD | ||||||
Region | 1-645 | Necessary and sufficient for the interaction with SQSTM1 | ||||
Sequence: MAQGSRRRKVVLTAGSEGWSPSSGPDMEELLRSVERDLNIDARQLALAPGGTHVVALVSTRWLASLRERRLGPCPRAEGLGEAEVRTLLQRSVQRLPPGWTRVEVHGLRKRRLSYPLGGGVPFEEGSCSPETLTRFMQEVAAQNYRNLWRHAYHTYGQPYSHSTAPSALPALDSIRQALQRVYGCTFLPVGESIPCLSNVRDGPCPSRGSPACPSLLRAEALLESPEMLYVVHPYVQFSLHDVVTFSPAKLTNSQAKVLFLLFRVLRAMDACHRQGLACGALSLHHIAVDEKLCSELRLDLSAYEMPSEDENQEGSEEKNGTGIKSEKEGEGRTECPTCQKELRGLVLDWVHGRISNFHYLMQLNRLAGRRQGDPNYHPVLPWVVDFTTPYGRFRDLRKSKFRLNKGDKQLDFTYEMTRQAFVAGGAGSGEPPHVPHHISDVLSDITYYVYKARRTPRSVLCGHVRAQWEPHEYPATMERMQTWTPDECIPEFYTDPSIFCSIHPDMPDLDVPAWCSSNQEFVAAHRALLESWEVSQDLHHWIDLTFGYKLQGKEAVKEKNVCLHLVDAHTHLTSYGVVQLFDQPHPQRLAGSPALAPEPPLIPRLLVQPIREATGQEDISGQLINGAGRLVVEATPCETGWTRD | ||||||
Region | 305-336 | Disordered | ||||
Sequence: EMPSEDENQEGSEEKNGTGIKSEKEGEGRTEC | ||||||
Compositional bias | 313-336 | Basic and acidic residues | ||||
Sequence: QEGSEEKNGTGIKSEKEGEGRTEC | ||||||
Domain | 335-612 | BEACH | ||||
Sequence: ECPTCQKELRGLVLDWVHGRISNFHYLMQLNRLAGRRQGDPNYHPVLPWVVDFTTPYGRFRDLRKSKFRLNKGDKQLDFTYEMTRQAFVAGGAGSGEPPHVPHHISDVLSDITYYVYKARRTPRSVLCGHVRAQWEPHEYPATMERMQTWTPDECIPEFYTDPSIFCSIHPDMPDLDVPAWCSSNQEFVAAHRALLESWEVSQDLHHWIDLTFGYKLQGKEAVKEKNVCLHLVDAHTHLTSYGVVQLFDQPHPQRLAGSPALAPEPPLIPRLLVQPIR | ||||||
Region | 665-717 | Disordered | ||||
Sequence: SLPGKAGDQPGSSSSQASPGLLSFSAPSGSRPGRRSKAAGLDPGEGEEGKIVL | ||||||
Compositional bias | 670-689 | Polar residues | ||||
Sequence: AGDQPGSSSSQASPGLLSFS | ||||||
Region | 1018-1044 | Disordered | ||||
Sequence: GKGLVGTTEDEESELPVSGPGSCAFGE | ||||||
Region | 1092-1175 | Disordered | ||||
Sequence: QPQEAEAVSLGQLSDKSSTSEASQGEERGGDDGGAPADKNSVKSGDSSQDLKQSEGSEEEEEEEGCVVLEEDQEDEVTGTSELT | ||||||
Compositional bias | 1096-1111 | Polar residues | ||||
Sequence: AEAVSLGQLSDKSSTS | ||||||
Compositional bias | 1146-1169 | Acidic residues | ||||
Sequence: EGSEEEEEEEGCVVLEEDQEDEVT | ||||||
Region | 1187-1209 | Disordered | ||||
Sequence: VAPGDGRDREEEEEPLTEQTEGK | ||||||
Region | 1515-1544 | Disordered | ||||
Sequence: SPSSRNPASMEPTMASAGPEWDPQSGSCLQ | ||||||
Compositional bias | 1562-1590 | Polar residues | ||||
Sequence: QIPDSQPQSPGPLGSLSGVGSSGGLSNRN | ||||||
Region | 1562-1599 | Disordered | ||||
Sequence: QIPDSQPQSPGPLGSLSGVGSSGGLSNRNEDNALKREL | ||||||
Repeat | 1639-1678 | WD 1 | ||||
Sequence: GHTGAVKCVAALSSEDFFLSGSKDRTVRLWPLYNYGDGTN | ||||||
Repeat | 1685-1725 | WD 2 | ||||
Sequence: IYAQHRKSVFYVGQLEAPQYVVSCDGAVHVWDPFTGKTLRT | ||||||
Repeat | 1777-1816 | WD 3 | ||||
Sequence: LNPGLVRSLAVSPSGRSVVAGFSSGFMVLLDTRTGLVLRG | ||||||
Repeat | 1819-1857 | WD 4 | ||||
Sequence: AHEGDILQIKAVEGSVLISSSSDHSLTVWKELEQKPTHH | ||||||
Repeat | 1904-1934 | WD 5 | ||||
Sequence: NFRGTLTSLALLPTKRHLLLGSDNGIIRLLA |
Sequence similarities
Belongs to the WD repeat WDR81 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing.
Q5ND34-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length1,934
- Mass (Da)211,945
- Last updated2012-09-05 v2
- Checksum8BE776A85B11C8AB
Q5ND34-2
- Name2
- Differences from canonical
- 1457-1490: Missing
Q5ND34-3
- Name3
- Differences from canonical
- 1769-1828: Missing
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Sequence caution
Features
Showing features for compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 313-336 | Basic and acidic residues | ||||
Sequence: QEGSEEKNGTGIKSEKEGEGRTEC | ||||||
Compositional bias | 670-689 | Polar residues | ||||
Sequence: AGDQPGSSSSQASPGLLSFS | ||||||
Compositional bias | 1096-1111 | Polar residues | ||||
Sequence: AEAVSLGQLSDKSSTS | ||||||
Compositional bias | 1146-1169 | Acidic residues | ||||
Sequence: EGSEEEEEEEGCVVLEEDQEDEVT | ||||||
Alternative sequence | VSP_019956 | 1457-1490 | in isoform 2 | |||
Sequence: Missing | ||||||
Compositional bias | 1562-1590 | Polar residues | ||||
Sequence: QIPDSQPQSPGPLGSLSGVGSSGGLSNRN | ||||||
Alternative sequence | VSP_019957 | 1769-1828 | in isoform 3 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL591496 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AK131146 EMBL· GenBank· DDBJ | BAD21396.1 EMBL· GenBank· DDBJ | mRNA | Sequence problems. | |
AK160585 EMBL· GenBank· DDBJ | BAE35892.1 EMBL· GenBank· DDBJ | mRNA | ||
AK172019 EMBL· GenBank· DDBJ | BAE42779.1 EMBL· GenBank· DDBJ | mRNA |