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Q5NAZ7 · GH33_ORYSJ

Function

function

Catalyzes the synthesis of jasmonate-amino acid conjugates by adenylation. Catalyzes the conjugation of jasmonate (JA) to Ile when expressed in a heterologous system (E.Coli) (PubMed:21619871).
Catalyzes in vitro the conjugation of jasmonate (JA) to Ile, Phe, Leu, Met, Val and Trp (PubMed:24033451).
May catalyze the synthesis of indole-3-acetic acid (IAA)-amino acid conjugates, providing a mechanism for the plant to cope with the presence of excess auxin (By similarity).

Catalytic activity

Features

Showing features for binding site.

146250100150200250300350400450
TypeIDPosition(s)Description
Binding site103ATP (UniProtKB | ChEBI)
Binding site106jasmonate (UniProtKB | ChEBI)
Binding site126ATP (UniProtKB | ChEBI)
Binding site170-174an L-alpha-amino acid (UniProtKB | ChEBI)
Binding site172ATP (UniProtKB | ChEBI)
Binding site334-337jasmonate (UniProtKB | ChEBI)
Binding site337-342ATP (UniProtKB | ChEBI)
Binding site339jasmonate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionacid-amino acid ligase activity
Molecular FunctionATP binding
Molecular Functionjasmonoyl-L-amino acid ligase activity
Biological Processresponse to auxin
Biological Processresponse to light stimulus

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Jasmonoyl--L-amino acid synthetase GH3.3
  • EC number
  • Alternative names
    • Auxin-responsive GH3-like protein 3
      (OsGH3-3
      )
    • Indole-3-acetic acid-amido synthetase GH3.3
    • Jasmonate-amino acid synthetase JAR2
    • Jasmonic acid-amido synthetase JAR2
    • Protein JASMONATE RESISTANT 2
      (OsJAR2
      )

Gene names

    • Name
      GH3.3
    • Synonyms
      JAR2
    • ORF names
      P0483F08.33-1
    • Ordered locus names
      Os01g0221100
      , LOC_Os01g12160

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Nipponbare
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa

Accessions

  • Primary accession
    Q5NAZ7
  • Secondary accessions
    • A0A0P0UZZ7
    • Q0JPI4

Proteomes

Genome annotation databases

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002035801-462Jasmonoyl--L-amino acid synthetase GH3.3

Proteomic databases

Expression

Tissue specificity

Expressed in green shoots and flowers.

Induction

At low level by auxin. Induced by wounding (PubMed:21619871).

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    462
  • Mass (Da)
    50,511
  • Last updated
    2005-11-22 v2
  • MD5 Checksum
    C7A6092853EFB510E9A8F4E7B7DCAEEF
MLEKKATRSTRVDGVSGEAVIEEFERVTRDAANVQRETLRRILAENGGVEYLRGLGLAGATDPATFRARVPLATHADLEPYIDRIADGDASPVLTAKPATSISLSSGTTQGKRKYLLFNEELVKSTMQIYRISYAFRNREFPVENGKALQFIYSSRETRTKGGLTATTATTNVYRSEEFKATMRDIQSQCCSPDEVIFGPDFAQSLYCHLLAGLLAAGDVQIVSATFAHSVVLAFQTFERAWEDLCADIRRGEVSPSRVTSPAVRRAMAALLAAPNPGLADEVARKCAALSNWYGVIPALWPNARYVYGIMTGSMEHYVKKLRHYAGGLPLVAAEYGASEGWVGANVEPGTPPERATFTVLPDIAYFEFIPLKPVAGDGGYAEAEPVGLTEVAAGELYEVVMTTFAGNTRSSSSCMTLVAYYYLQSKKWMNICRFCISVHETSRNLVTCTFAATGHIMFAQK

Sequence caution

The sequence BAD81367.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict362in Ref. 5; AK072125

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP002094
EMBL· GenBank· DDBJ
BAD81367.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AP008207
EMBL· GenBank· DDBJ
BAF04344.1
EMBL· GenBank· DDBJ
Genomic DNA
AP014957
EMBL· GenBank· DDBJ
BAS71074.1
EMBL· GenBank· DDBJ
Genomic DNA
AK072125
EMBL· GenBank· DDBJ
-mRNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

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