Q5NAZ7 · GH33_ORYSJ
- ProteinJasmonoyl--L-amino acid synthetase GH3.3
- GeneGH3.3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids462 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the synthesis of jasmonate-amino acid conjugates by adenylation. Catalyzes the conjugation of jasmonate (JA) to Ile when expressed in a heterologous system (E.Coli) (PubMed:21619871).
Catalyzes in vitro the conjugation of jasmonate (JA) to Ile, Phe, Leu, Met, Val and Trp (PubMed:24033451).
May catalyze the synthesis of indole-3-acetic acid (IAA)-amino acid conjugates, providing a mechanism for the plant to cope with the presence of excess auxin (By similarity).
Catalyzes in vitro the conjugation of jasmonate (JA) to Ile, Phe, Leu, Met, Val and Trp (PubMed:24033451).
May catalyze the synthesis of indole-3-acetic acid (IAA)-amino acid conjugates, providing a mechanism for the plant to cope with the presence of excess auxin (By similarity).
Catalytic activity
- a jasmonate + an L-alpha-amino acid + ATP = a jasmonyl-L-amino acid + AMP + diphosphate + H+
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 103 | ATP (UniProtKB | ChEBI) | |||
Binding site | 106 | jasmonate (UniProtKB | ChEBI) | |||
Binding site | 126 | ATP (UniProtKB | ChEBI) | |||
Binding site | 170-174 | an L-alpha-amino acid (UniProtKB | ChEBI) | |||
Binding site | 172 | ATP (UniProtKB | ChEBI) | |||
Binding site | 334-337 | jasmonate (UniProtKB | ChEBI) | |||
Binding site | 337-342 | ATP (UniProtKB | ChEBI) | |||
Binding site | 339 | jasmonate (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | acid-amino acid ligase activity | |
Molecular Function | ATP binding | |
Molecular Function | jasmonoyl-L-amino acid ligase activity | |
Biological Process | response to auxin | |
Biological Process | response to light stimulus |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameJasmonoyl--L-amino acid synthetase GH3.3
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ5NAZ7
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000203580 | 1-462 | Jasmonoyl--L-amino acid synthetase GH3.3 | ||
Proteomic databases
Expression
Tissue specificity
Expressed in green shoots and flowers.
Induction
At low level by auxin. Induced by wounding (PubMed:21619871).
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Sequence similarities
Belongs to the IAA-amido conjugating enzyme family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length462
- Mass (Da)50,511
- Last updated2005-11-22 v2
- MD5 ChecksumC7A6092853EFB510E9A8F4E7B7DCAEEF
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 362 | in Ref. 5; AK072125 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP002094 EMBL· GenBank· DDBJ | BAD81367.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AP008207 EMBL· GenBank· DDBJ | BAF04344.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP014957 EMBL· GenBank· DDBJ | BAS71074.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK072125 EMBL· GenBank· DDBJ | - | mRNA | No translation available. |