Q5M7Y0 · CERT_DANRE
- ProteinCeramide transfer protein
- Genecert1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids620 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Shelters ceramides and diacylglycerol lipids inside its START domain and mediates the intracellular trafficking of ceramides and diacylglycerol lipids in a non-vesicular manner (By similarity).
Isoform 1 plays a role in neural development and skeletal muscle development. Negatively regulates apoptosis during development.
Isoform 1 plays a role in neural development and skeletal muscle development. Negatively regulates apoptosis during development.
Catalytic activity
- N-hexadecanoylsphing-4-enine(in) = N-hexadecanoylsphing-4-enine(out)
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 468 | an N-acylsphing-4-enine (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 489 | an N-acylsphing-4-enine (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 526 | an N-acylsphing-4-enine (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 575 | an N-acylsphing-4-enine (UniProtKB | ChEBI) | ||||
Sequence: Y |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | endoplasmic reticulum | |
Cellular Component | Golgi apparatus | |
Molecular Function | ceramide 1-phosphate binding | |
Molecular Function | ceramide 1-phosphate transfer activity | |
Molecular Function | ceramide binding | |
Molecular Function | ceramide transfer activity | |
Biological Process | ceramide transport | |
Biological Process | ER to Golgi ceramide transport | |
Biological Process | intermembrane lipid transfer | |
Biological Process | muscle organ development |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCeramide transfer protein
- Short namesCERT
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio
Accessions
- Primary accessionQ5M7Y0
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Preferentially localized to the Golgi apparatus.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 64 | Fails to rescue the increased-apoptosis phenotype, probably by abolishing ceramide transfer between the endoplasmic reticulum and Golgi complex by disrupting phosphatidylinositol 4-phosphate-binding activity. | ||||
Sequence: G → E |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000307356 | 1-620 | Ceramide transfer protein | |||
Sequence: MSDCSSSGSDEDLDPESELLDELGGKLSKWTNYIHGWQDRWIALKGNILSYYRSADEKEYGCRGSICLSKAVITPHEFDECRFDISVNDSIWYLRAEHPLLRQQWIDTIELHKAESGYGSESSLRRHGSLLSLTSAASGLSTASASSFKKAYSLQEKLAEMETFRDILCRQVDTLQKYFDNCADNESKDELHRDKVSDEEEDFPTLRPDADYLLNNNNSSKEKLFSVNTLKDPAGIDFKGEAITFKATTAGILSTLSHCIDILVKREESWQKRLDKEIERRRRAEDAYKAALTELKKKPHYGGPDYEEGPNSLINEEEFFDAVEAALDKHDQIEEQSQVERSRASRQISLTPDTFSSISTQKYLTKPHSHTSSLSSVDLISASDDVHRFSAQVEEMVHSHMTYSLQDVGGDANWQLVVEEGDMKVYRREVEENGIVLDPLKATHAVKGVTGHEVCHYFWDTAYRNDWETTVESFQVVETLSDQACIIHQTLKRVWPASQRDVLYVSVMRKILSTNENDPDTWLVCNFSVDHDGYPPSTRCIRAKINVAMICQTLISPPEGDKEISRDNIICKITYVANVNPGGWAPASVLRAVAKREYPKFLKRFTSYVEVKTSSEAILF |
Proteomic databases
Expression
Tissue specificity
Widely expressed during embryogenesis.
Developmental stage
The isoforms are differentially expressed during development. Isoform 1 is expressed maternally with expression gradually decreasing as development progresses, whereas expression of isoform 2 is barely detectable during the first 12 hours post-fertilization (hpf) but increases by the second day of development.
Interaction
Subunit
Interacts with VAPA and VAPB. Interaction with VAPB is less efficient than with VAPA. Interacts (via FFAT motif) with the MOSPD2 (via MSP domain).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, coiled coil, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 20-114 | PH | ||||
Sequence: LDELGGKLSKWTNYIHGWQDRWIALKGNILSYYRSADEKEYGCRGSICLSKAVITPHEFDECRFDISVNDSIWYLRAEHPLLRQQWIDTIELHKA | ||||||
Coiled coil | 265-298 | |||||
Sequence: KREESWQKRLDKEIERRRRAEDAYKAALTELKKK | ||||||
Motif | 318-324 | FFAT | ||||
Sequence: EFFDAVE | ||||||
Domain | 385-614 | START | ||||
Sequence: DVHRFSAQVEEMVHSHMTYSLQDVGGDANWQLVVEEGDMKVYRREVEENGIVLDPLKATHAVKGVTGHEVCHYFWDTAYRNDWETTVESFQVVETLSDQACIIHQTLKRVWPASQRDVLYVSVMRKILSTNENDPDTWLVCNFSVDHDGYPPSTRCIRAKINVAMICQTLISPPEGDKEISRDNIICKITYVANVNPGGWAPASVLRAVAKREYPKFLKRFTSYVEVKTS |
Domain
The START domain recognizes ceramides and diacylglycerol lipids, interacts with membranes, and mediates the intermembrane transfer of ceramides and diacylglycerol lipids.
The FFAT motif is required for interaction with VAPA, VAPB and MOSPD2.
The PH domain targets the Golgi apparatus.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing.
Q5M7Y0-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- SynonymsGpbp
- Length620
- Mass (Da)70,374
- Last updated2009-09-22 v2
- ChecksumD2485F872847F354
Q5M7Y0-2
- Name2
- SynonymsGpbpD26, GpbpDelta26, GpbpDelta26/CERT
- Differences from canonical
- 367-392: Missing
Q5M7Y0-3
- Name3
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A2R9YJF5 | A0A2R9YJF5_DANRE | cert1a | 594 | ||
F8W2V5 | F8W2V5_DANRE | cert1a | 620 |
Sequence caution
Features
Showing features for alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_038154 | 1-241 | in isoform 3 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_038155 | 242-276 | in isoform 3 | |||
Sequence: AITFKATTAGILSTLSHCIDILVKREESWQKRLDK → MTTVCSASVVNWVSGSLRSANENWIPLCTQSSFFT | ||||||
Alternative sequence | VSP_038156 | 367-392 | in isoform 2 | |||
Sequence: Missing | ||||||
Sequence conflict | 385 | in Ref. 1; ABV60275 | ||||
Sequence: D → E |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
EU000165 EMBL· GenBank· DDBJ | ABV60275.1 EMBL· GenBank· DDBJ | mRNA | ||
EU000166 EMBL· GenBank· DDBJ | ABV60276.1 EMBL· GenBank· DDBJ | mRNA | ||
BX511209 EMBL· GenBank· DDBJ | CAM13175.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
BX511209 EMBL· GenBank· DDBJ | CAM13176.1 EMBL· GenBank· DDBJ | Genomic DNA |