Q5M7Y0 · CERT_DANRE

Function

function

Shelters ceramides and diacylglycerol lipids inside its START domain and mediates the intracellular trafficking of ceramides and diacylglycerol lipids in a non-vesicular manner (By similarity).
Isoform 1 plays a role in neural development and skeletal muscle development. Negatively regulates apoptosis during development.

Catalytic activity

Features

Showing features for binding site.

162050100150200250300350400450500550600
TypeIDPosition(s)Description
Binding site468an N-acylsphing-4-enine (UniProtKB | ChEBI)
Binding site489an N-acylsphing-4-enine (UniProtKB | ChEBI)
Binding site526an N-acylsphing-4-enine (UniProtKB | ChEBI)
Binding site575an N-acylsphing-4-enine (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytosol
Cellular Componentendoplasmic reticulum
Cellular ComponentGolgi apparatus
Molecular Functionceramide 1-phosphate binding
Molecular Functionceramide 1-phosphate transfer activity
Molecular Functionceramide binding
Molecular Functionceramide transfer activity
Biological Processceramide transport
Biological ProcessER to Golgi ceramide transport
Biological Processintermembrane lipid transfer
Biological Processmuscle organ development

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ceramide transfer protein
  • Short names
    CERT
  • Alternative names
    • Collagen type IV alpha-3-binding protein
    • Goodpasture antigen-binding protein (GPBP)

Gene names

    • Name
      cert1
    • Synonyms
      cert, col4a3bp, gpbp
    • ORF names
      si:ch211-129a6.6

Organism names

  • Taxonomic identifier
  • Strain
    • Tuebingen
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio

Accessions

  • Primary accession
    Q5M7Y0
  • Secondary accessions
    • A2BGX3
    • A2BGX4
    • B4XAM8
    • B4XAM9

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Golgi apparatus
Endoplasmic reticulum
Note: Preferentially localized to the Golgi apparatus.

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis64Fails to rescue the increased-apoptosis phenotype, probably by abolishing ceramide transfer between the endoplasmic reticulum and Golgi complex by disrupting phosphatidylinositol 4-phosphate-binding activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003073561-620Ceramide transfer protein

Proteomic databases

Expression

Tissue specificity

Widely expressed during embryogenesis.

Developmental stage

The isoforms are differentially expressed during development. Isoform 1 is expressed maternally with expression gradually decreasing as development progresses, whereas expression of isoform 2 is barely detectable during the first 12 hours post-fertilization (hpf) but increases by the second day of development.

Interaction

Subunit

Interacts with VAPA and VAPB. Interaction with VAPB is less efficient than with VAPA. Interacts (via FFAT motif) with the MOSPD2 (via MSP domain).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, coiled coil, motif.

TypeIDPosition(s)Description
Domain20-114PH
Coiled coil265-298
Motif318-324FFAT
Domain385-614START

Domain

The START domain recognizes ceramides and diacylglycerol lipids, interacts with membranes, and mediates the intermembrane transfer of ceramides and diacylglycerol lipids.
The FFAT motif is required for interaction with VAPA, VAPB and MOSPD2.
The PH domain targets the Golgi apparatus.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete

This entry describes 3 isoforms produced by Alternative splicing.

Q5M7Y0-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    1
  • Synonyms
    Gpbp
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    620
  • Mass (Da)
    70,374
  • Last updated
    2009-09-22 v2
  • Checksum
    D2485F872847F354
MSDCSSSGSDEDLDPESELLDELGGKLSKWTNYIHGWQDRWIALKGNILSYYRSADEKEYGCRGSICLSKAVITPHEFDECRFDISVNDSIWYLRAEHPLLRQQWIDTIELHKAESGYGSESSLRRHGSLLSLTSAASGLSTASASSFKKAYSLQEKLAEMETFRDILCRQVDTLQKYFDNCADNESKDELHRDKVSDEEEDFPTLRPDADYLLNNNNSSKEKLFSVNTLKDPAGIDFKGEAITFKATTAGILSTLSHCIDILVKREESWQKRLDKEIERRRRAEDAYKAALTELKKKPHYGGPDYEEGPNSLINEEEFFDAVEAALDKHDQIEEQSQVERSRASRQISLTPDTFSSISTQKYLTKPHSHTSSLSSVDLISASDDVHRFSAQVEEMVHSHMTYSLQDVGGDANWQLVVEEGDMKVYRREVEENGIVLDPLKATHAVKGVTGHEVCHYFWDTAYRNDWETTVESFQVVETLSDQACIIHQTLKRVWPASQRDVLYVSVMRKILSTNENDPDTWLVCNFSVDHDGYPPSTRCIRAKINVAMICQTLISPPEGDKEISRDNIICKITYVANVNPGGWAPASVLRAVAKREYPKFLKRFTSYVEVKTSSEAILF

Q5M7Y0-2

  • Name
    2
  • Synonyms
    GpbpD26, GpbpDelta26, GpbpDelta26/CERT
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Q5M7Y0-3

  • Name
    3
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-241: Missing
    • 242-276: AITFKATTAGILSTLSHCIDILVKREESWQKRLDK → MTTVCSASVVNWVSGSLRSANENWIPLCTQSSFFT

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A2R9YJF5A0A2R9YJF5_DANREcert1a594
F8W2V5F8W2V5_DANREcert1a620

Sequence caution

The sequence CAM13175.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for alternative sequence, sequence conflict.

TypeIDPosition(s)Description
Alternative sequenceVSP_0381541-241in isoform 3
Alternative sequenceVSP_038155242-276in isoform 3
Alternative sequenceVSP_038156367-392in isoform 2
Sequence conflict385in Ref. 1; ABV60275

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
EU000165
EMBL· GenBank· DDBJ
ABV60275.1
EMBL· GenBank· DDBJ
mRNA
EU000166
EMBL· GenBank· DDBJ
ABV60276.1
EMBL· GenBank· DDBJ
mRNA
BX511209
EMBL· GenBank· DDBJ
CAM13175.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
BX511209
EMBL· GenBank· DDBJ
CAM13176.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp