Q5LW89 · DDDW_RUEPO

Function

function

Able to cleave dimethylsulfoniopropionate (DMSP), releasing dimethyl sulfide (DMS) and acrylate. DMS is the principal form by which sulfur is transported from oceans to the atmosphere.

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ ion per subunit.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
8.68 μMdimethylsulfoniopropionatein the presence of Fe2+
4.5 μMdimethylsulfoniopropionatein the presence of Mn2+
kcat is 18.25 sec-1 with dimethylsulfoniopropionate (in the presence of Fe2+).
kcat is 17.33 sec-1 with dimethylsulfoniopropionate (in the presence of Mn2+).

pH Dependence

Optimum pH is 8.0.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site83Fe cation (UniProtKB | ChEBI)
Binding site87Fe cation (UniProtKB | ChEBI)
Binding site89Fe cation (UniProtKB | ChEBI)
Binding site121Fe cation (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functiondimethylpropiothetin dethiomethylase activity
Molecular Functionmetal ion binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Dimethylsulfoniopropionate lyase DddW
  • EC number
  • Short names
    DMSP lyase

Gene names

    • Name
      dddW
    • Ordered locus names
      SPO0453

Organism names

Accessions

  • Primary accession
    Q5LW89

Proteomes

Phenotypes & Variants

Disruption phenotype

Decreased production of dimethyl sulfide (DMS).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis81Decreased iron binding and enzyme activity toward DMSP.
Mutagenesis83Decreased iron binding and enzyme activity toward DMSP.
Mutagenesis87Decreased iron binding and enzyme activity toward DMSP.
Mutagenesis121Decreased iron binding and enzyme activity toward DMSP.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004339001-152Dimethylsulfoniopropionate lyase DddW

Proteomic databases

Expression

Induction

Up-regulated by pre-growth of cells with dimethylsulfoniopropionate (DMSP).

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain69-124Cupin type-2

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    152
  • Mass (Da)
    16,132
  • Last updated
    2005-02-01 v1
  • Checksum
    90EF8584AEFCA325
MTAMLDSFATDLTAATSLHQPGNLPHPPHAIDAENVPLSGGTDPTYGEVRWRTLINGTEAAPRDMVLGIAEFGPGHQLRPHRHTPPEFYLGLEGSGIVTIDGVPHEIRAGVALYIPGDAEHGTVAGPEGLRFAYGFASASFEAIEYRFTASA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000031
EMBL· GenBank· DDBJ
AAV93771.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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