Q5L199 · Q5L199_GEOKA
- ProteinPhosphoenolpyruvate-protein phosphotransferase
- GeneptsI
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids573 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).
Catalytic activity
- L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-L-histidyl-[protein] + pyruvate
Cofactor
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 190 | Tele-phosphohistidine intermediate | ||||
Sequence: H | ||||||
Binding site | 297 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 333 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 432 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 455-456 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: ND | ||||||
Binding site | 456 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 466 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Active site | 503 | Proton donor | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | kinase activity | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoenolpyruvate-protein phosphotransferase activity | |
Biological Process | phosphoenolpyruvate-dependent sugar phosphotransferase system |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoenolpyruvate-protein phosphotransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Geobacillus > Geobacillus thermoleovorans group
Accessions
- Primary accessionQ5L199
Proteomes
Subcellular Location
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 6-127 | Phosphotransferase system enzyme I N-terminal | ||||
Sequence: HGIAASSGIAIAKAYRLETPHLAAEKRTVADVEAEIARLEAAVAKAKEELEAIKQHALEKLGEDKAAIFAAHLLVLDDPELLNPIKEKIKTEQVNAEYALHETASFFISMFEGMDNEYMKER | ||||||
Coiled coil | 27-61 | |||||
Sequence: LAAEKRTVADVEAEIARLEAAVAKAKEELEAIKQH | ||||||
Domain | 154-226 | PEP-utilising enzyme mobile | ||||
Sequence: SEEVVIIAEDLTPSDTAQLNRQYVKGFATDIGGRTSHSAIMARSLEIPAVVGTKAITAEVQNGDIVIVDGLDG | ||||||
Domain | 251-541 | PEP-utilising enzyme C-terminal | ||||
Sequence: KAEWAKLVDQPTVTADGVHVELAANIGTPDDVKGALANGAEGIGLYRTEFLYMGRSELPTEDEQFAAYKTVLEQMGGKPVVVRTLDIGGDKELPYLHLPKEMNPFLGFRAIRLCLEMQDMFRTQLRALLRASVHGNLKIMFPMIATLDEFRQAKAILLEEKEALLRQGVPVADGIEVGMMVEIPAAAVMADQFAREVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRLISYVIDAAHREGKWVGMCGEMAGDPIAIPILLALGLDEFSMSATSILPARAQLKRL |
Sequence similarities
Belongs to the PEP-utilizing enzyme family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length573
- Mass (Da)63,021
- Last updated2005-02-01 v1
- ChecksumD1E72B9FDA0A273E
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BA000043 EMBL· GenBank· DDBJ | BAD75281.1 EMBL· GenBank· DDBJ | Genomic DNA |