Q5IBH7 · SPDYA_MOUSE
- ProteinSpeedy protein A
- GeneSpdya
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids310 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2 (PubMed:15611625).
Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation. Interferes with CDKN1B-mediated inhibition of CDK2. Mediates cell survival during the DNA damage process through activation of CDK2 (By similarity).
Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation. Interferes with CDKN1B-mediated inhibition of CDK2. Mediates cell survival during the DNA damage process through activation of CDK2 (By similarity).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chromosome, telomeric region | |
Cellular Component | nuclear envelope | |
Cellular Component | nucleoplasm | |
Cellular Component | nucleus | |
Cellular Component | XY body | |
Molecular Function | protein kinase activator activity | |
Molecular Function | protein kinase binding | |
Biological Process | DNA damage response | |
Biological Process | establishment of protein localization to telomere | |
Biological Process | G1/S transition of mitotic cell cycle | |
Biological Process | male meiotic nuclear division | |
Biological Process | meiotic attachment of telomere to nuclear envelope | |
Biological Process | oogenesis | |
Biological Process | positive regulation of cell population proliferation | |
Biological Process | positive regulation of cyclin-dependent protein serine/threonine kinase activity | |
Biological Process | spermatogenesis | |
Biological Process | telomere capping |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameSpeedy protein A
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ5IBH7
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 89 | Abolishes interaction with CDK2. | ||||
Sequence: D → A | ||||||
Mutagenesis | 102 | Abolishes interaction with CDK2. | ||||
Sequence: M → A | ||||||
Mutagenesis | 106 | Abolishes interaction with CDK2. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 107 | Abolishes interaction with CDK2. | ||||
Sequence: F → A | ||||||
Mutagenesis | 133-135 | No effect on CDK2-binding. | ||||
Sequence: EED → AAA |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 16 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000234113 | 1-310 | Speedy protein A | |||
Sequence: MRHNQMYCETPPTVTIHVKSGSNRSHQTRKPISLKRPILKDSWEASENNAQNNKSKRPRGPCLIIQRQEMTAFFKLFDDDLIQDFLWMDCCCKIADKYLLAMTFVYFKRAKFTINEHTRINFFIALYLANTVEEDEEEAKYEIFPWALGKNWRKLFPNFLKLRDQLWDRIDYRAIVSRRCCEEVMAIAPTHYIWQRERSVHHSGAVRNYNRDEVHLPRGPSATPVDCSLCGKKGRYVRLGLSSSSSSSSDTGELMEKDSQELHSAFSVDTAGDPPHTYSQVANDHQSNKENETNFVKKNKSVEWFAESEE | ||||||
Modified residue | 221 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 223 | Phosphothreonine | ||||
Sequence: T |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed at a high level in testis. Also expressed in the adult ovary and in immature oocytes.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 67-199 | Speedy/Ringo box; Required for CDK-binding | ||||
Sequence: RQEMTAFFKLFDDDLIQDFLWMDCCCKIADKYLLAMTFVYFKRAKFTINEHTRINFFIALYLANTVEEDEEEAKYEIFPWALGKNWRKLFPNFLKLRDQLWDRIDYRAIVSRRCCEEVMAIAPTHYIWQRERS | ||||||
Region | 241-310 | Disordered | ||||
Sequence: LSSSSSSSSDTGELMEKDSQELHSAFSVDTAGDPPHTYSQVANDHQSNKENETNFVKKNKSVEWFAESEE | ||||||
Compositional bias | 273-289 | Polar residues | ||||
Sequence: DPPHTYSQVANDHQSNK | ||||||
Compositional bias | 290-310 | Basic and acidic residues | ||||
Sequence: ENETNFVKKNKSVEWFAESEE |
Domain
The C-terminus is required for CDK2-activation, but not CDK2-binding.
Sequence similarities
Belongs to the Speedy/Ringo family.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q5IBH7-2
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name2
- SynonymsA2
- Length310
- Mass (Da)36,115
- Last updated2011-07-27 v2
- Checksum10F372690FF03826
Q5IBH7-1
- Name1
- SynonymsA1
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
B8JJB9 | B8JJB9_MOUSE | Spdya | 309 |
Features
Showing features for sequence conflict, compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 258 | in Ref. 1; AAW32476/AAW32477 | ||||
Sequence: D → G | ||||||
Compositional bias | 273-289 | Polar residues | ||||
Sequence: DPPHTYSQVANDHQSNK | ||||||
Alternative sequence | VSP_052029 | 281-283 | in isoform 1 | |||
Sequence: VAN → GMA | ||||||
Alternative sequence | VSP_052030 | 284-310 | in isoform 1 | |||
Sequence: Missing | ||||||
Compositional bias | 290-310 | Basic and acidic residues | ||||
Sequence: ENETNFVKKNKSVEWFAESEE |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY820306 EMBL· GenBank· DDBJ | AAW32476.1 EMBL· GenBank· DDBJ | mRNA | ||
AY820307 EMBL· GenBank· DDBJ | AAW32477.1 EMBL· GenBank· DDBJ | mRNA | ||
AK077138 EMBL· GenBank· DDBJ | BAC36637.1 EMBL· GenBank· DDBJ | mRNA | ||
CT010429 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |