Q5I7T1 · AG10B_HUMAN

  • Protein
    Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
  • Gene
    ALG10B
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Dol-P-Glc:Glc2Man9GlcNAc2-PP-Dol alpha-1,2-glucosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the third and last glucose residue from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide intermediate Glc2Man9GlcNAc2-PP-Dol to produce Glc3Man9GlcNAc2-PP-Dol.

Catalytic activity

Pathway

Protein modification; protein glycosylation.

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum
Cellular Componentendoplasmic reticulum membrane
Cellular Componentlumenal side of endoplasmic reticulum membrane
Cellular Componentplasma membrane
Molecular Functiondolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity
Biological Processdolichol-linked oligosaccharide biosynthetic process
Biological Processprotein N-linked glycosylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
  • EC number
  • Alternative names
    • Alpha-1,2-glucosyltransferase ALG10-A
    • Alpha-2-glucosyltransferase ALG10-B
    • Asparagine-linked glycosylation protein 10 homolog B
    • Potassium channel regulator 1

Gene names

    • Name
      ALG10B
    • Synonyms
      KCR1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo

Accessions

  • Primary accession
    Q5I7T1
  • Secondary accessions
    • B2RPF4

Proteomes

Organism-specific databases

Subcellular Location

Endoplasmic reticulum membrane
; Multi-pass membrane protein
Note: Also detected at the plasma membrane.

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-6Cytoplasmic
Transmembrane7-27Helical
Topological domain28-64Extracellular
Transmembrane65-85Helical
Topological domain86-97Cytoplasmic
Transmembrane98-118Helical
Topological domain119-126Extracellular
Transmembrane127-147Helical
Topological domain148-150Cytoplasmic
Transmembrane151-171Helical
Topological domain172-175Extracellular
Transmembrane176-196Helical
Topological domain197-256Cytoplasmic
Transmembrane257-277Helical
Topological domain278-283Extracellular
Transmembrane284-304Helical
Topological domain305-317Cytoplasmic
Transmembrane318-338Helical
Topological domain339-365Extracellular
Transmembrane366-386Helical
Topological domain387-392Cytoplasmic
Transmembrane393-413Helical
Topological domain414-436Extracellular
Transmembrane437-457Helical
Topological domain458-473Cytoplasmic

Keywords

Disease & Variants

Features

Showing features for natural variant.

TypeIDPosition(s)Description
Natural variantVAR_08908633found in a patient with ventricular fibrillation and QT prolongation; uncertain significance; dbSNP:rs188043534
Natural variantVAR_04821784in dbSNP:rs6582584
Natural variantVAR_061002383in dbSNP:rs57963306
Natural variantVAR_023753446in dbSNP:rs61730283

Variants

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The viewer provides 613 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Organism-specific databases

Miscellaneous

Genetic variation databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002154481-473Dol-P-Glc:Glc2Man9GlcNAc2-PP-Dol alpha-1,2-glucosyltransferase

Proteomic databases

PTM databases

Expression

Tissue specificity

Highly expressed in heart, placenta, liver, kidney and pancreas. Weakly expressed in lung, skeletal muscle and brain.

Gene expression databases

Organism-specific databases

Interaction

Subunit

Interacts with KCNH1; may regulate KCNH1, possibly by regulating its N-glycosylation. Interacts with KCNH2; may reduce KCNH2 sensitivity to classic proarrhythmic drug blockade, possibly by regulating its N-glycosylation.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q5I7T1CD79A P119123EBI-18075734, EBI-7797864
BINARY Q5I7T1SHISAL1 Q3SXP73EBI-18075734, EBI-18037857

Protein-protein interaction databases

Miscellaneous

Family & Domains

Sequence similarities

Belongs to the ALG10 glucosyltransferase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    473
  • Mass (Da)
    55,448
  • Last updated
    2010-05-18 v2
  • Checksum
    47D0CF7B6C84B8EC
MAQLEGYCFSAALSCTFLVSCLLFSAFSRALREPYMDEIFHLPQAQRYCEGHFSLSQWDPMITTLPGLYLVSVGVVKPAIWIFAWSEHVVCSIGMLRFVNLLFSVGNFYLLYLLFHKVQPRNKAASSIQRVLSTLTLAVFPTLYFFNFLYYTEAGSMFFTLFAYLMCLYGNHKTSAFLGFCGFMFRQTNIIWAVFCAGNVIAQKLTEAWKTELQKKEDRLPPIKGPFAEFRKILQFLLAYSMSFKNLSMLFCLTWPYILLGFLFCAFVVVNGGIVIGDRSSHEACLHFPQLFYFFSFTLFFSFPHLLSPSKIKTFLSLVWKHGILFLVVTLVSVFLVWKFTYAHKYLLADNRHYTFYVWKRVFQRYAILKYLLVPAYIFAGWSIADSLKSKPIFWNLMFFICLFIVIVPQKLLEFRYFILPYVIYRLNITLPPTSRLVCELSCYAIVNFITFYIFLNKTFQWPNSQDIQRFMW

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
F8VXJ0F8VXJ0_HUMANALG10B126
F8VWA9F8VWA9_HUMANALG10B77

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY845858
EMBL· GenBank· DDBJ
AAW31756.1
EMBL· GenBank· DDBJ
mRNA
AC117372
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CH471111
EMBL· GenBank· DDBJ
EAW57795.1
EMBL· GenBank· DDBJ
Genomic DNA
BC137413
EMBL· GenBank· DDBJ
AAI37414.1
EMBL· GenBank· DDBJ
mRNA
BC137414
EMBL· GenBank· DDBJ
AAI37415.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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