Q5I050 · NUP1B_XENLA
- ProteinCytosolic Fe-S cluster assembly factor nubp1-B
- Genenubp1-B
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids315 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The nubp1-nubp2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.
Cofactor
Note: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of nubp1 and two labile, bridging clusters between subunits of the nubp1-nubp2 heterotetramer.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 12 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 26 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 29 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 35 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 66-73 | ATP (UniProtKB | ChEBI) | |||
Binding site | 239 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); ligand shared with heterodimeric partner | |||
Binding site | 242 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); ligand shared with heterodimeric partner | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | ATP binding | |
Molecular Function | ATP-dependent FeS chaperone activity | |
Molecular Function | iron-sulfur cluster binding | |
Molecular Function | metal ion binding | |
Biological Process | iron-sulfur cluster assembly |
Keywords
- Ligand
Names & Taxonomy
Protein names
- Recommended nameCytosolic Fe-S cluster assembly factor nubp1-B
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Xenopus
Accessions
- Primary accessionQ5I050
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000382594 | 1-315 | Cytosolic Fe-S cluster assembly factor nubp1-B | ||
Expression
Gene expression databases
Interaction
Subunit
Heterotetramer of 2 nubp1 and 2 nubp2 chains.
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length315
- Mass (Da)33,704
- Last updated2005-02-15 v1
- Checksum4FF321470DE5E9E1
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC088708 EMBL· GenBank· DDBJ | AAH88708.1 EMBL· GenBank· DDBJ | mRNA |